Structure of the glucan-binding sugar chain of Tip1p, a cell wall protein of Saccharomyces cerevisiae

• Published in: Biochimica et biophysica acta Vol. 1427; no. 2; pp. 133 - 144
• Main Authors: Fujii, Tsutomu, Shimoi, Hitoshi, Iimura, Yuzuru
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 19.04.1999
• Subjects: Amino Acid Sequence; Amino Acids; Amino Acids - analysis; analysis; Carbohydrate Sequence; carbohydrate structure; Carrier Proteins; Carrier Proteins - chemistry; Carrier Proteins - isolation & purification; Carrier Proteins - metabolism; cell wall components; Cell wall glucan; Cell wall protein; chemical structure; chemistry; Endopeptidases; Ethanolamine; Ethanolamine - analysis; glucans; Glucans - metabolism; glycoproteins; Glycoproteins - chemistry; Glycoproteins - isolation & purification; Glycoproteins - metabolism; GPI anchor; Hexoses; Hexoses - analysis; isolation & purification; Membrane Glycoproteins; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - isolation & purification; Membrane Glycoproteins - metabolism; metabolism; Molecular Sequence Data; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Cell wall glucan; ABOE, 4-aminobenzoic octyl ester; PA, 2-aminopyridine; GPI, glycosylphosphatidylinositol; SDS, sodium dodecyl sulfate; HF, hydrofluoric acid; AQC, N-hydroxysuccinimidyl-6-aminoquinolinyl carbamate; GPI anchor; CBB, Coomassie Brilliant Blue R-250; PAGE, polyacrylamide gel electrophoresis; Saccharomyces cerevisiae; Cell wall protein; TFA, trifluoroacetic acid

Tip1p is one of the major cell wall mannoproteins of Saccharomyces cerevisiae and is presumed to be synthesized as a glycosylphosphatidylinositol (GPI)-anchored form. We purified Tip1p from a glucanase extract of yeast cell walls and analyzed the sugar chain involved in the cell wall linkage. One mo...