Structure of the PCBP2/stem–loop IV complex underlying translation initiation mediated by the poliovirus type I IRES

• Published in: Nucleic acids research Vol. 48; no. 14; pp. 8006 - 8021
• Main Authors: Beckham, Simone A, Matak, Mehdi Y, Belousoff, Matthew J, Venugopal, Hariprasad, Shah, Neelam, Vankadari, Naveen, Elmlund, Hans, Nguyen, Joseph H C, Semler, Bert L, Wilce, Matthew C J, Wilce, Jacqueline A
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 20.08.2020
• Subjects: Cryoelectron Microscopy; Models, Molecular; Nucleic Acid Conformation; Peptide Chain Initiation, Translational; Poliovirus - genetics; Protein Conformation; RNA and RNA-protein complexes; RNA, Viral - chemistry; RNA, Viral - metabolism; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - metabolism; Scattering, Small Angle; X-Ray Diffraction

Abstract The poliovirus type I IRES is able to recruit ribosomal machinery only in the presence of host factor PCBP2 that binds to stem–loop IV of the IRES. When PCBP2 is cleaved in its linker region by viral proteinase 3CD, translation initiation ceases allowing the next stage of replication to com...