Development of an enzyme-linked immunosorbent assay for the measurement of plasma growth hormone (GH) levels in channel catfish ( Ictalurus punctatus): assessment of environmental salinity and GH secretogogues on plasma GH levels

• Published in: General and comparative endocrinology Vol. 133; no. 3; pp. 314 - 322
• Main Authors: Drennon, Katherine, Moriyama, Shunsuke, Kawauchi, Hiroshi, Small, Brian, Silverstein, Jeffrey, Parhar, Ishwar, Shepherd, Brian
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2003
• Subjects: animal growth; Animals; bioassays; blood plasma; Brackish; catfish; Cattle; Channel catfish; dose response; Dose-Response Relationship, Drug; ELISA; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; fish culture; Fresh Water - chemistry; Freshwater; GHRH; GHRP-2; Growth hormone; Growth Hormone - blood; Growth Hormone - drug effects; Growth Hormone - immunology; Growth Hormone-Releasing Hormone - agonists; Growth Hormone-Releasing Hormone - blood; Growth Hormone-Releasing Hormone - pharmacology; hormone secretion; hyperosmotic conditions; Ictaluridae - blood; Ictalurus punctatus; in vivo studies; Oligopeptides; osmoregulation; Pituitary Gland - cytology; Pituitary Gland - drug effects; Pituitary Gland - immunology; Prolactin - blood; Rabbits; Salinity; Seawater - chemistry; somatotropin; Teleostei; GHRP-2; Channel catfish; Growth hormone; GHRH; ELISA; Salinity

We report the development of a sensitive, and specific, competitive, antigen-capture enzyme-linked immunosorbent assay for the measurement of channel catfish ( Ictalurus punctatus) growth hormone (cfGH). The detection limit of the assay (90% binding) was 2.0 ng/ml and the ED 50 value (standard curve...