Online trapping and enrichment ultra performance liquid chromatography–tandem mass spectrometry method for sensitive measurement of “arginine-asymmetric dimethylarginine cycle” biomarkers in human exhaled breath condensate

• Published in: Analytica chimica acta Vol. 754; pp. 67 - 74
• Main Authors: Di Gangi, Iole Maria, Pirillo, Paola, Carraro, Silvia, Gucciardi, Antonina, Naturale, Mauro, Baraldi, Eugenio, Giordano, Giuseppe
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19.11.2012; Elsevier
• Subjects: ADMA; Adolescent; adolescents; adults; Analytical chemistry; Arginine - analogs & derivatives; Arginine - analysis; Arginine - metabolism; Arginine-ADMA cycle; asthma; Asthma - diagnosis; Asthma - drug therapy; Asthma - metabolism; biomarkers; Biomarkers - analysis; Breath Tests; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid; correlation; cystic fibrosis; detection limit; Exact sciences and technology; Exhaled breath condensate (EBC); Female; Humans; Internet; liquid chromatography; Male; metabolites; monitoring; NOS; Other chromatographic methods; Sensitivity and Specificity; spectrometers; Spectrometric and optical methods; Tandem Mass Spectrometry; Arginine-ADMA cycle; Tandem mass spectrometry; NOS; EBC; ICS; ADMA; UPLC–MS/MS; Exhaled breath condensate (EBC); COPD; Performance evaluation; Chemical analysis; On line; Metabolite; Bias; Biological marker; Chemical enrichment; Accuracy; Arginine; Standard curve; Adult; Robustness; Monitoring; Quantitative analysis; Correlation coefficient; Human; Sample; Use; Isotope dilution; Cystic fibrosis; Liquid chromatography; Concentration; High pressure; Objective; Trapping; Asymmetry; Mass spectrometry MS/MS; Simultaneous measurement; Analytical method; Measurement method

[Display omitted] ► Simultaneous quantification of “arginine-ADMA cycle” metabolites developed in EBC. ► EBC is a non-invasive matrix highly useful in patients with respiratory diseases. ► Method, fast, precise and accurate, is suitable in the pediatric clinical studies. ► Sensitivity is increased u...