The Lung Tissue Microbiome in Chronic Obstructive Pulmonary Disease
Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others. To extend these findings to human lung tissue samples. DNA f...
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| Published in | American journal of respiratory and critical care medicine Vol. 185; no. 10; pp. 1073 - 1080 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
New York, NY
American Thoracic Society
15.05.2012
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others.
To extend these findings to human lung tissue samples.
DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing.
Total bacterial populations within lung tissue were small (20-1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group (P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups (P < 0.003) attributable to an increase in the Lactobacillus genus (P < 0.0007).
There is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD. |
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| AbstractList | Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others. To extend these findings to human lung tissue samples. DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing. Total bacterial populations within lung tissue were small (20-1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group (P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups (P < 0.003) attributable to an increase in the Lactobacillus genus (P < 0.0007). There is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD. Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others.RATIONALEBased on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others.To extend these findings to human lung tissue samples.OBJECTIVESTo extend these findings to human lung tissue samples.DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing.METHODSDNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing.Total bacterial populations within lung tissue were small (20-1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group (P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups (P < 0.003) attributable to an increase in the Lactobacillus genus (P < 0.0007).MEASUREMENT AND MAIN RESULTSTotal bacterial populations within lung tissue were small (20-1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group (P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups (P < 0.003) attributable to an increase in the Lactobacillus genus (P < 0.0007).There is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD.CONCLUSIONSThere is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD. Rationale : Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others. Objectives : To extend these findings to human lung tissue samples. Methods : DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing. Measurement and Main Results : Total bacterial populations within lung tissue were small (20–1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group ( P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups ( P < 0.003) attributable to an increase in the Lactobacillus genus ( P < 0.0007). Conclusions : There is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD. Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and chronic obstructive pulmonary disease (COPD), which have now been confirmed by others. To extend these findings to human lung tissue samples. DNA from lung tissue samples was obtained from nonsmokers (n = 8); smokers without COPD (n = 8); patients with very severe COPD (Global Initiative for COPD [GOLD] 4) (n = 8); and patients with cystic fibrosis (CF) (n = 8). The latter served as a positive control, with sterile water as a negative control. All bacterial community analyses were based on polymerase chain reaction amplifying 16S rRNA gene fragments. Total bacterial populations were measured by quantitative polymerase chain reaction and bacterial community composition was assessed by terminal restriction fragment length polymorphism analysis and pyrotag sequencing. Total bacterial populations within lung tissue were small (20-1,252 bacterial cells per 1,000 human cells) but greater in all four sample groups versus the negative control group (P < 0.001). Terminal restriction fragment length polymorphism analysis and sequencing distinguished three distinct bacterial community compositions: one common to the nonsmoker and smoker groups, a second to the GOLD 4 group, and the third to the CF-positive control group. Pyrotag sequencing identified greater than 1,400 unique bacterial sequences and showed an increase in the Firmicutes phylum in GOLD 4 patients versus all other groups (P < 0.003) attributable to an increase in the Lactobacillus genus (P < 0.0007). There is a detectable bacterial community within human lung tissue that changes in patients with very severe COPD. |
| Author | McDonough, John E. Hogg, James C. Cooper, Joel Hayashi, Shizu Sin, Don D. Mohn, William W. Sze, Marc A. Gosselink, John V. Elliott, W. Mark Dimitriu, Pedro A. |
| Author_xml | – sequence: 1 givenname: Marc A. surname: Sze fullname: Sze, Marc A. organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 2 givenname: Pedro A. surname: Dimitriu fullname: Dimitriu, Pedro A. organization: Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada; and – sequence: 3 givenname: Shizu surname: Hayashi fullname: Hayashi, Shizu organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 4 givenname: W. Mark surname: Elliott fullname: Elliott, W. Mark organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 5 givenname: John E. surname: McDonough fullname: McDonough, John E. organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 6 givenname: John V. surname: Gosselink fullname: Gosselink, John V. organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 7 givenname: Joel surname: Cooper fullname: Cooper, Joel organization: Department of Cardiovascular and Thoracic Surgery, University of Pennsylvania, Philadelphia, Pennsylvania – sequence: 8 givenname: Don D. surname: Sin fullname: Sin, Don D. organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and – sequence: 9 givenname: William W. surname: Mohn fullname: Mohn, William W. organization: Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada; and – sequence: 10 givenname: James C. surname: Hogg fullname: Hogg, James C. organization: The James Hogg Research Centre, Providence Heart-Lung Institute at St. Paul's Hospital, Departments of Medicine and Pathology and Laboratory Medicine, and |
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| Snippet | Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of asthma and... Rationale : Based on surface brushings and bronchoalveolar lavage fluid, Hilty and coworkers demonstrated microbiomes in the human lung characteristic of... |
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| SubjectTerms | Adult Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Bacteria Biological and medical sciences Case-Control Studies Chronic obstructive pulmonary disease Chronic obstructive pulmonary disease, asthma Cystic fibrosis Cystic Fibrosis - microbiology DNA, Bacterial - analysis Female Humans Intensive care medicine Lavage Lung - microbiology Lungs Male Medical sciences Metagenome Middle Aged Pneumology Polymerase Chain Reaction Polymorphism Polymorphism, Restriction Fragment Length Principal Component Analysis Pulmonary Disease, Chronic Obstructive - microbiology RNA, Ribosomal, 16S Sequence Analysis, DNA Severity of Illness Index Smoking Taxonomy Transplants & implants |
| Title | The Lung Tissue Microbiome in Chronic Obstructive Pulmonary Disease |
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