Accuracy assessment on the analysis of unbound drug in plasma by comparing traditional centrifugal ultrafiltration with hollow fiber centrifugal ultrafiltration and application in pharmacokinetic study

• Published in: Journal of Chromatography A Vol. 1318; pp. 265 - 269
• Main Authors: Zhang, Lin, Zhang, Zhi-Qing, Dong, Wei-Chong, Jing, Shao-Jun, Zhang, Jin-Feng, Jiang, Ye
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 29.11.2013; Elsevier
• Subjects: Analysis; Biological and medical sciences; Centrifugation - methods; Centrifugation ultrafiltration; chromatography; detection limit; General pharmacology; Hollow fiber centrifugation ultrafiltration; HPLC; Humans; Limit of Detection; Medical sciences; Metformin; Pharmaceutical Preparations - blood; Pharmaceutical Preparations - chemistry; Pharmacokinetics; Pharmacology. Drug treatments; ultrafiltration; Ultrafiltration - instrumentation; Ultrafiltration - methods; Centrifugation ultrafiltration; Hollow fiber centrifugation ultrafiltration; Metformin; HPLC; Drug; Biological fluid; Chemical analysis; Healthy subject; Free form; HPLC chromatography; Blood; Blood plasma; Hypoglycemic agent; Accuracy; Biguanides; Ultraviolet detector; Sample preparation; Hollow fiber; Centrifugal Ultrafiltration; Comparative study; Quantitative analysis

•Factors that affect the ultrafiltrate volume were comprehensively investigated.•Ultrafiltrate volume is a crucial factor on the accurate measurement of unbound drug.•The ultrafiltrate volume by HFCF-UF can be easily controlled.•The HFCF-UF method has extremely low detection limit and perfect recove...