Transcriptome Profiling Reveals Differential Gene Expression during the Process of Microtuber Formation in Pinellia ternata

• Published in: International journal of molecular sciences Vol. 24; no. 14; p. 11604
• Main Authors: Bo, Chen, Su, Chuandong, Teng, Jingtong, Sheng, Wei, Xue, Tao, Zhu, Yanfang, Xue, Jianping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.07.2023; MDPI
• Subjects: differentially expressed genes (DEGs); expression pattern; Gene expression; microtuber; Pinellia ternata; Plant growth; RNA-seq; Signal transduction; Transcription factors; Trends; Pinellia ternata; differentially expressed genes (DEGs); expression pattern; RNA-seq; microtuber
• DOI: 10.3390/ijms241411604
• ISSN: 1422-0067; 1661-6596

Using petiole material as explants and directly inducing the formation of microtubers without going through the callus stage is an essential way to rapidly expand scarce medical plants such as . However, the early molecular mechanism underlying the formation of the microtuber is largely elusive. Here, we conducted cytology and dynamic transcriptome analyses of inchoate microtubers in explants and identified 1092 differentially expressed genes after their cultivation in vitro for 0, 5, and 15 days. Compared with 0 day, the number and size of the microtuber cells were larger at 5 and 15 days of culture. Detailed categorization revealed that the differentially expressed genes were mainly related to responses to stimulus, biological regulation, organelles, membranes, transcription factor activity, and protein binding. Further analysis revealed that the microtuber at different incubation days exhibited quite a difference in both hormone signaling pathway transduction and the regulation pattern of transcription factors. Therefore, this study contributes to a better understanding of the early molecular regulation during the formation of the microtuber and provides new insights for the study of the rapid expansion of and other medical plants.