Growth signalobody selects functional intrabodies in the mammalian cytoplasm

• Published in: Biotechnology journal Vol. 11; no. 4; p. 565
• Main Authors: Lee, Songhee, Kaku, Yoshihiro, Inoue, Satoshi, Nagamune, Teruyuki, Kawahara, Masahiro
• Format: Journal Article
• Language: English
• Published: Germany 01.04.2016
• Subjects: Animals; Antigens - metabolism; Cell Line; Cell Proliferation; Cytoplasm - metabolism; Humans; Interleukin-3 - pharmacology; Mice; Peptide Library; Protein Engineering - methods; Protein Multimerization; Protein-Tyrosine Kinases - chemistry; Protein-Tyrosine Kinases - genetics; Protein-Tyrosine Kinases - metabolism; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Signal Transduction; Single-Chain Antibodies - genetics; Single-Chain Antibodies - metabolism; Receptor tyrosine kinase; Library selection; Mammalian cell; Intrabody; Growth signalobody

A versatile strategy to inhibit protein functions in the cytoplasmic environment is eagerly anticipated for drug discovery. In this study, we demonstrate a novel system to directly select functional intrabodies from a library in the mammalian cytoplasm. In this system, a target homo-oligomeric antig...