Structural Characterization of Nuclear Poly(A)‐Protein Particles in Rat Liver

• Published in: European journal of biochemistry Vol. 131; no. 2; pp. 283 - 288
• Main Authors: TOMCSÁNYI, Tihamér, MOLNÁR, János, TIGYI, András
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1983; Blackwell
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell Nucleus - analysis; Centrifugation, Density Gradient; Chemical Phenomena; Chemistry; Fundamental and applied biological sciences. Psychology; liver; Liver - analysis; Micrococcal Nuclease; nuclei; Nucleic acids; Nucleoproteins - isolation & purification; Poly A - isolation & purification; Rats; ribonucleoproteins; Rna, ribonucleoproteins; Ribonucleoprotein; Animal

Poly(A)‐protein particles were prepared from rat liver nuclear extract after digestion with pancreatic ribonuclease and ribonuclease T1 by sucrose gradient centrifugation. The particles were sedimented in a range of 9–23 S with a peak at 16 S. The particles isolated in this manner were 99–100% resis...