HSP101 functions as a specific translational regulatory protein whose activity is regulated by nutrient status

• Published in: Genes & development Vol. 12; no. 20; pp. 3236 - 3251
• Main Authors: Wells, Douglas R., Tanguay, Robert L., Le, Hanh, Gallie, Daniel R.
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 15.10.1998
• Subjects: Amino Acid Sequence; Cell Respiration - genetics; Eukaryotic Initiation Factor-3; Eukaryotic Initiation Factor-4G; Heat-Shock Proteins - chemistry; Heat-Shock Proteins - genetics; Heat-Shock Proteins - physiology; Molecular Sequence Data; Nicotiana - genetics; Nicotiana - metabolism; Peptide Initiation Factors - genetics; Peptide Initiation Factors - physiology; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - physiology; Plants, Toxic; Poly A - metabolism; Protein Biosynthesis; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - physiology; Research Paper; RNA, Messenger - metabolism; Tobacco mosaic virus; Transcription Factors - chemistry; Transcription Factors - genetics; Transcription Factors - physiology; Triticum - genetics; Triticum - metabolism

The 5′ leader (Ω) of tobacco mosaic viral RNA functions as a translational enhancer. Sequence analysis of a 102-kD protein, identified previously as a specific Ω RNA-binding protein, revealed homology to the HSP101/HSP104/ClpB family of heat shock proteins and its expression in yeast complemented a...