High resolution annotation of zebrafish transcriptome using long-read sequencing

• Published in: Genome research Vol. 28; no. 9; pp. 1415 - 1425
• Main Authors: Nudelman, German, Frasca, Antonio, Kent, Brandon, Sadler, Kirsten C., Sealfon, Stuart C., Walsh, Martin J., Zaslavsky, Elena
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.09.2018
• Subjects: Alternative splicing; Animals; Annotations; Computer applications; Conserved sequence; Danio rerio; Embryos; Gene expression; Genomes; Homology; Isoforms; Molecular Sequence Annotation; Resource; Sequence Analysis, RNA - methods; Sequence Analysis, RNA - standards; Sequence Homology, Nucleic Acid; SMRT protein; Transcription; Transcriptome; Zebrafish; Zebrafish - genetics

With the emergence of zebrafish as an important model organism, a concerted effort has been made to study its transcriptome. This effort is limited, however, by gaps in zebrafish annotation, which are especially pronounced concerning transcripts dynamically expressed during zygotic genome activation...