Establishment of a Reverse Genetic System from a Bovine Derived Influenza D Virus Isolate

• Published in: Viruses Vol. 13; no. 3; p. 502
• Main Authors: Holwerda, Melle, Laloli, Laura, Wider, Manon, Schönecker, Lutz, Becker, Jens, Meylan, Mireille, Dijkman, Ronald
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 18.03.2021; MDPI AG
• Subjects: IDV; influenza D virus; minireplicon assay; reverse genetics; influenza D virus; reverse genetics; IDV; minireplicon assay

The ruminant-associated influenza D virus (IDV) has a broad host tropism and was shown to have zoonotic potential. To identify and characterize molecular viral determinants influencing the host spectrum of IDV, a reverse genetic system is required. For this, we first performed 5' and 3' ra...