Proteomic and Transcriptomic Analysis Identify Spliceosome as a Significant Component of the Molecular Machinery in the Pituitary Tumors Derived from POU1F1 - and NR5A1 -Cell Lineages

• Published in: Genes Vol. 11; no. 12; p. 1422
• Main Authors: Taniguchi-Ponciano, Keiko, Peña-Martínez, Eduardo, Silva-Román, Gloria, Vela-Patiño, Sandra, Guzman-Ortiz, Ana Laura, Quezada, Hector, Gomez-Apo, Erick, Chavez-Macias, Laura, Mercado-Medrez, Sophia, Vargas-Ortega, Guadalupe, Espinosa-de-Los-Monteros, Ana Laura, Gonzales-Virla, Baldomero, Ferreira-Hermosillo, Aldo, Espinosa-Cardenas, Etual, Ramirez-Renteria, Claudia, Sosa, Ernesto, Lopez-Felix, Blas, Guinto, Gerardo, Marrero-Rodríguez, Daniel, Mercado, Moises
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.11.2020; MDPI
• Subjects: alternative splicing; Brain cancer; Brain tumors; Central nervous system; DNA microarrays; Gene expression; Gene regulation; High-performance liquid chromatography; Isoforms; Mass spectroscopy; molecular markers; mRNA isoforms; Peptides; Pituitary; pituitary adenomas; Proteins; Proteomics; Splicing; Transcriptomics; Tumorigenesis; Tumors; United States > US; alternative splicing; mRNA isoforms; molecular markers; pituitary adenomas
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes11121422

Pituitary adenomas (PA) are the second most common tumor in the central nervous system and have low counts of mutated genes. Splicing occurs in 95% of the coding RNA. There is scarce information about the spliceosome and mRNA-isoforms in PA, and therefore we carried out proteomic and transcriptomic analysis to identify spliceosome components and mRNA isoforms in PA. Proteomic profile analysis was carried out by nano-HPLC and mass spectrometry with a quadrupole time-of-flight mass spectrometer. The mRNA isoforms and transcriptomic profiles were carried out by microarray technology. With proteins and mRNA information we carried out Gene Ontology and exon level analysis to identify splicing-related events. Approximately 2000 proteins were identified in pituitary tumors. Spliceosome proteins such as , and among others were found in PA. These results were validated at mRNA level, which showed up-regulation of spliceosome genes in PA. Spliceosome-related genes segregate and categorize PA tumor subtypes. The PA showed alterations in and mRNA isoforms which could be tumor specific. Spliceosome components are significant constituents of the PA molecular machinery and could be used as molecular markers and therapeutic targets. Splicing-related genes and mRNA-isoforms profiles characterize tumor subtypes.