Characterization of 15 polymorphic microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae), a conifer of medicinal importance

Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against le...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of molecular sciences Vol. 13; no. 9; pp. 11165 - 11172
Main Authors Miao, Yingchun, Lang, Xuedong, Li, Shuaifeng, Su, Jianrong, Wang, Yuehua
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.09.2012
Molecular Diversity Preservation International (MDPI)
Subjects
Alleles
Amplified Fragment Length Polymorphism Analysis - methods
Cephalotaxus - genetics
Cephalotaxus oliveri
Cloning
DNA, Plant - genetics
FIASCO
Genetics, Population
Habitats
microsatellite loci
Microsatellite Repeats - genetics
PCR amplification
Plants, Medicinal - genetics
Polymorphism, Genetic
Population genetics
Predation
Seeds
Short Note
Vietnam
China
microsatellite loci
Cephalotaxus oliveri
PCR amplification
FIASCO
Online AccessGet full text

Cover

Abstract Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species.
AbstractList Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species.
Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri , 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri , which will be useful for establishing effective conservation strategies for this species.
Author Su, Jianrong
Miao, Yingchun
Wang, Yuehua
Lang, Xuedong
Li, Shuaifeng
AuthorAffiliation 2 Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Kunming 650224, China; E-Mails: langxuedong@gmail.com (X.L.); shuaifengli@163.com (S.L.)
1 Department of Botany, School of Life Sciences, Yunnan University, Kunming 650091, China; E-Mail: myczsh@163.com
AuthorAffiliation_xml – name: 2 Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Kunming 650224, China; E-Mails: langxuedong@gmail.com (X.L.); shuaifengli@163.com (S.L.)
– name: 1 Department of Botany, School of Life Sciences, Yunnan University, Kunming 650091, China; E-Mail: myczsh@163.com
Author_xml – sequence: 1
  givenname: Yingchun
  surname: Miao
  fullname: Miao, Yingchun
  email: myczsh@163.com, langxuedong@gmail.com, shuaifengli@163.com
  organization: Department of Botany, School of Life Sciences, Yunnan University, Kunming 650091, China; E-Mail: myczsh@163.com ; Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Kunming 650224, China; E-Mails: langxuedong@gmail.com (X.L.); shuaifengli@163.com (S.L.)
– sequence: 2
  givenname: Xuedong
  surname: Lang
  fullname: Lang, Xuedong
– sequence: 3
  givenname: Shuaifeng
  surname: Li
  fullname: Li, Shuaifeng
– sequence: 4
  givenname: Jianrong
  surname: Su
  fullname: Su, Jianrong
– sequence: 5
  givenname: Yuehua
  surname: Wang
  fullname: Wang, Yuehua
BackLink https://www.ncbi.nlm.nih.gov/pubmed/23109844$$D View this record in MEDLINE/PubMed
BookMark eNpdks9rFDEUx4NU7A89epWAlwqu5sckmVwEWawWCl70HN5kkm6WzGRMZor17B9utltr11PCex8-5PvyTtHRmEaH0EtK3nGuyfuwHQrlRFNKpXiCTmjD2IoQqY4e3Y_RaSlbQhhnQj9Dx4xTotumOUG_1xvIYGeXwy-YQxpx8pgKPKV4O6Q8bYLFQ7A5FZhdjGF2OCYbsE8Zr920gZhm-LkUnGK4qRJ8_q8K1oF78xYDtmkM3uWde3B9sGGEiMMwpTzDaN1z9NRDLO7F_XmGvl98-rb-srr6-vly_fFqZZuWzquOA7PEO2k1l8r3rRWSKtv3nWCSE2WtZ1Z2kvecMiG17nvllW-1VkQL3_EzdLn39gm2ZsphgHxrEgRzV0j52kCeg43OMEllx1vhQTYNUKVbxzRwLwWj1nU714e9a1q6msm6cc4QD6SHnTFszHW6MbxRTHFZBef3gpx-LK7MZgjF1hnD6NJSDKWsUUIpzir6-j90m5ZcZ1ipGp0wRhpaqdWe2v1Wyc4_PIYSs9sVc7ArlX_1OMED_Xc5-B_osb6B
CitedBy_id crossref_primary_10_3390_f13091452
crossref_primary_10_1016_j_bioorg_2020_104445
crossref_primary_10_3389_fgene_2021_759557
crossref_primary_10_3390_ijms22020787
crossref_primary_10_3732_apps_1500129
crossref_primary_10_1051_e3sconf_202126501030
crossref_primary_10_1371_journal_pone_0103789
Cites_doi 10.1093/molbev/msl191
10.1023/A:1017083025154
10.1139/x01-198
10.1177/117693430500100003
10.1111/j.1471-8286.2007.01931.x
10.1139/G08-033
10.1046/j.0962-1083.2001.01418.x
10.1159/000121069
10.1111/j.1365-294X.2011.05396.x
10.1111/j.1601-5223.2004.01804.x
10.1007/s00122-004-1871-x
10.1111/j.1755-0998.2011.03014.x
10.3732/ajb.1100128
ContentType Journal Article
Copyright Copyright MDPI AG 2012
2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. 2012
Copyright_xml – notice: Copyright MDPI AG 2012
– notice: 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. 2012
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7X7
7XB
88E
8FI
8FJ
8FK
8G5
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
GUQSH
K9.
M0S
M1P
M2O
MBDVC
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
7X8
5PM
DOA
DOI 10.3390/ijms130911165
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
ProQuest_Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Medical Database (Alumni Edition)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Research Library (Alumni Edition)
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
AUTh Library subscriptions: ProQuest Central
ProQuest One Community College
ProQuest Central Korea
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
Research Library Prep
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
PML(ProQuest Medical Library)
Research Library
Research Library (Corporate)
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
MEDLINE - Academic
PubMed Central (Full Participant titles)
Directory of Open Access Journals
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
Research Library Prep
ProQuest Central Student
ProQuest Central Essentials
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
Research Library (Alumni Edition)
ProQuest Central China
ProQuest Central
Health Research Premium Collection
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
ProQuest Research Library
ProQuest Medical Library (Alumni)
ProQuest Central Basic
ProQuest One Academic Eastern Edition
ProQuest Hospital Collection
Health Research Premium Collection (Alumni)
ProQuest Hospital Collection (Alumni)
ProQuest Health & Medical Complete
ProQuest Medical Library
ProQuest One Academic UKI Edition
ProQuest One Academic
ProQuest Central (Alumni)
MEDLINE - Academic
DatabaseTitleList Publicly Available Content Database
CrossRef


MEDLINE
Database_xml – sequence: 1
  dbid: DOA
  name: Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 1422-0067
EndPage 11172
ExternalDocumentID oai_doaj_org_article_2616b385fa644a1798e29a3f6521cebb
3308636181
10_3390_ijms130911165
23109844
Genre Research Support, Non-U.S. Gov't
Journal Article
GeographicLocations Vietnam
China
GeographicLocations_xml – name: Vietnam
– name: China
GroupedDBID ---
29J
2WC
3V.
53G
5GY
5VS
7X7
88E
8FE
8FG
8FH
8FI
8FJ
8G5
A8Z
AADQD
AAFWJ
AAHBH
ABDBF
ABJCF
ABUWG
ACGFO
ACIHN
ACIWK
ACPRK
ADBBV
ADRAZ
AEAQA
AENEX
AFKRA
AFZYC
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
BPHCQ
BVXVI
C1A
CCPQU
CGR
CS3
CUY
CVF
D1I
DIK
DU5
DWQXO
E3Z
EBD
EBS
ECM
EIF
EJD
ESTFP
ESX
F5P
FRP
FYUFA
GNUQQ
GROUPED_DOAJ
GUQSH
GX1
HCIFZ
HH5
HMCUK
HYE
IAO
IPNFZ
ITC
KB.
KQ8
LK8
M1P
M2O
M48
M7P
MODMG
M~E
NPM
O5R
O5S
OK1
P2P
PDBOC
PIMPY
PQQKQ
PROAC
PSQYO
RIG
RNS
RPM
TR2
TUS
UKHRP
~8M
AAYXX
AFPKN
CITATION
7XB
8FK
K9.
MBDVC
PQEST
PQUKI
PRINS
Q9U
7X8
5PM
ID FETCH-LOGICAL-c481t-b3a2c0fe6c9367fd8c5617cddb526307ccf2c6b63d3125699dd7f7f8997095fb3
IEDL.DBID RPM
ISSN 1422-0067
1661-6596
IngestDate Tue Oct 22 15:14:13 EDT 2024
Tue Sep 17 21:22:10 EDT 2024
Fri Oct 25 11:34:34 EDT 2024
Thu Oct 10 19:42:24 EDT 2024
Thu Sep 26 16:12:30 EDT 2024
Tue Oct 15 23:44:01 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 9
Keywords microsatellite loci
Cephalotaxus oliveri
PCR amplification
FIASCO
Language English
License This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c481t-b3a2c0fe6c9367fd8c5617cddb526307ccf2c6b63d3125699dd7f7f8997095fb3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3472736/
PMID 23109844
PQID 1526022041
PQPubID 2032341
PageCount 8
ParticipantIDs doaj_primary_oai_doaj_org_article_2616b385fa644a1798e29a3f6521cebb
pubmedcentral_primary_oai_pubmedcentral_nih_gov_3472736
proquest_miscellaneous_1124757732
proquest_journals_1526022041
crossref_primary_10_3390_ijms130911165
pubmed_primary_23109844
PublicationCentury 2000
PublicationDate 2012-09-01
PublicationDateYYYYMMDD 2012-09-01
PublicationDate_xml – month: 09
  year: 2012
  text: 2012-09-01
  day: 01
PublicationDecade 2010
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle International journal of molecular sciences
PublicationTitleAlternate Int J Mol Sci
PublicationYear 2012
Publisher MDPI AG
Molecular Diversity Preservation International (MDPI)
Publisher_xml – name: MDPI AG
– name: Molecular Diversity Preservation International (MDPI)
References 11903900 - Mol Ecol. 2002 Jan;11(1):1-16
21821588 - Am J Bot. 2011 Aug;98(8):e229-32
9720288 - Genetica. 1998;102-103(1-6):359-67
17150975 - Mol Biol Evol. 2007 Mar;24(3):621-31
18545277 - Genome. 2008 Jul;51(7):534-46
21585727 - Mol Ecol Resour. 2008 Jan;8(1):103-6
19325852 - Evol Bioinform Online. 2007 Feb 23;1:47-50
21565126 - Mol Ecol Resour. 2011 Jul;11(4):591-611
15655666 - Theor Appl Genet. 2005 Feb;110(3):550-60
18504349 - Cytogenet Genome Res. 2008;120(3-4):210-9
15383073 - Hereditas. 2004;141(1):61-7
22151648 - Mol Ecol. 2012 Jan;21(2):314-28
Excoffier (ref_17) 2005; 1
Zane (ref_13) 2002; 11
Collignon (ref_14) 2002; 32
Song (ref_11) 2005; 110
Pan (ref_7) 2011; 98
Zhang (ref_9) 2008; 51
Llorens (ref_5) 2012; 21
Schug (ref_10) 1998; 102–103
Cuadrado (ref_12) 2008; 120
Guichoux (ref_6) 2011; 11
ref_2
Chapuis (ref_19) 2007; 24
Fu (ref_1) 1984; 22
ref_16
ref_15
Koskinen (ref_8) 2004; 141
Zhu (ref_3) 1979; 17
ref_4
Rousset (ref_18) 2008; 8
References_xml – volume: 17
  start-page: 7
  year: 1979
  ident: ref_3
  article-title: The types of alkaloids in plants of the genus Cephalotaxus (in Chinese)
  publication-title: Acta. Phytotaxonomica. Sinica
  contributor:
    fullname: Zhu
– volume: 24
  start-page: 621
  year: 2007
  ident: ref_19
  article-title: Microsatellite null alleles and estimation of population differentiation
  publication-title: Mol. Biol. Evol.
  doi: 10.1093/molbev/msl191
  contributor:
    fullname: Chapuis
– ident: ref_4
– volume: 102–103
  start-page: 359
  year: 1998
  ident: ref_10
  article-title: Mutation and evolution of microsatellites inDrosophila melanogaster
  publication-title: Genetica
  doi: 10.1023/A:1017083025154
  contributor:
    fullname: Schug
– volume: 32
  start-page: 266
  year: 2002
  ident: ref_14
  article-title: Geographical variation in random amplified polymorphic DNA and quantitative traits in Norway spruce
  publication-title: Can. J. For. Res
  doi: 10.1139/x01-198
  contributor:
    fullname: Collignon
– ident: ref_2
– volume: 1
  start-page: 47
  year: 2005
  ident: ref_17
  article-title: Arlequin (version. 3.0): An integrated software package for population genetics data analysis
  publication-title: Evol. Bioinform. Online
  doi: 10.1177/117693430500100003
  contributor:
    fullname: Excoffier
– volume: 8
  start-page: 103
  year: 2008
  ident: ref_18
  article-title: GenePop’007: A complete re-implementation of the GenePop software for Windows and Linux
  publication-title: Mol. Ecol. Resour
  doi: 10.1111/j.1471-8286.2007.01931.x
  contributor:
    fullname: Rousset
– volume: 22
  start-page: 277
  year: 1984
  ident: ref_1
  article-title: The study within Cephalotaxus (in Chinese)
  publication-title: J. Syst. Evol
  contributor:
    fullname: Fu
– volume: 51
  start-page: 534
  year: 2008
  ident: ref_9
  article-title: Characteristics and analysis of simple sequence repeats in the cotton genome based on a linkage map constructed from a BC1 population between Gossypium hirsutum and G. barbadense
  publication-title: Genome
  doi: 10.1139/G08-033
  contributor:
    fullname: Zhang
– ident: ref_16
– volume: 11
  start-page: 1
  year: 2002
  ident: ref_13
  article-title: Strategies for microsatellite isolation: A review
  publication-title: Mol. Ecol
  doi: 10.1046/j.0962-1083.2001.01418.x
  contributor:
    fullname: Zane
– ident: ref_15
– volume: 120
  start-page: 210
  year: 2008
  ident: ref_12
  article-title: Physical organisation of simple sequence repeats (SSRs) in Triticeae: Structural, functional and evolutionary implications
  publication-title: Cytogenet. Genome Res
  doi: 10.1159/000121069
  contributor:
    fullname: Cuadrado
– volume: 21
  start-page: 314
  year: 2012
  ident: ref_5
  article-title: Evaluating the influence of different aspects of habitat fragmentation on mating patterns and pollen dispersal in the bird-pollinated Banksia sphaerocarpa var. caesia
  publication-title: Mol. Ecol
  doi: 10.1111/j.1365-294X.2011.05396.x
  contributor:
    fullname: Llorens
– volume: 141
  start-page: 61
  year: 2004
  ident: ref_8
  article-title: The benefits of increasing the number of microsatellites utilized in genetic population studies: An empirical perspective
  publication-title: Hereditas
  doi: 10.1111/j.1601-5223.2004.01804.x
  contributor:
    fullname: Koskinen
– volume: 110
  start-page: 550
  year: 2005
  ident: ref_11
  article-title: Development and mapping of microsatellite (SSR) markers in wheat
  publication-title: Theor. Appl. Genet
  doi: 10.1007/s00122-004-1871-x
  contributor:
    fullname: Song
– volume: 11
  start-page: 591
  year: 2011
  ident: ref_6
  article-title: Current trends in microsatellite genotyping
  publication-title: Mol. Ecol. Resour
  doi: 10.1111/j.1755-0998.2011.03014.x
  contributor:
    fullname: Guichoux
– volume: 98
  start-page: e229
  year: 2011
  ident: ref_7
  article-title: Development of microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae) and cross-amplification in Cephalotaxus
  publication-title: Am. J. Bot
  doi: 10.3732/ajb.1100128
  contributor:
    fullname: Pan
SSID ssj0023259
Score 2.0789797
Snippet Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due...
Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due...
SourceID doaj
pubmedcentral
proquest
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 11165
SubjectTerms Alleles
Amplified Fragment Length Polymorphism Analysis - methods
Cephalotaxus - genetics
Cephalotaxus oliveri
Cloning
DNA, Plant - genetics
FIASCO
Genetics, Population
Habitats
microsatellite loci
Microsatellite Repeats - genetics
PCR amplification
Plants, Medicinal - genetics
Polymorphism, Genetic
Population genetics
Predation
Seeds
Short Note
SummonAdditionalLinks – databaseName: Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3faxQxEB5KQeiLWK262pYIRRRcervJJpvH9rDU0hMfLPRtyU96ct0t3h7Ud__wTpK9650Ivvi6CSGbmcl8k0y-ATgyzDilqM8rVtqcIWTOpShc7p1WrDCj2ttwoD_5ys-v2MV1db1W6ivkhCV64LRwx4jwuaZ15RV6bhXotVwpcXCOfsc4rePuO5LLYGoItWgZy6SFA448bMiJXZNigH88_XE7x407GHnwKGveKJL2_w1p_pkwueaBzp7B0wE6kpM05V3Ycu1zeJKKSf56Ab_HK-7l9LSSdJ4UFfnWzTC-x-WcGjIJ6XdzFVk4e0cuUTIEUSsZu7sbNet6db-Yk24WcjWm5MPjV4VL7T5-IoqMu5gME8aepGt5nNKX24jiUX_24Ors8_fxeT7UWMgNq4s-11SVZuQdN5Jy4W1tEFAJY62uSo72b4wvDdecWopQiEtprfDCY5QmEJx5TV_Cdtu17jUQZYSWuEUYqymzttDCGVo7SWWlNcKcDN4v17q5S1QaDYYgQSjNhlAyOA2SWHUKDNjxA-pFM-hF8y-9yGB_KcdmMMt5g2CFh6fFrMjg3aoZDSrckqjWdQvsg4hHVELQMoNXSeyrmQQwLGvGMhAbCrEx1c2WdnoTSbspC0iRv_kf__YWdhC3lSnVbR-2-58Ld4DYqNeH0QweAHmbDiA
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: AUTh Library subscriptions: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9RAEB_0iuCL-G20ygoiCoZespvd7JPYo6WKV4pY6FvYT3tyzZ5NDvTdP9zZJJf2RHzdDWGYz9_szs4AvDLMOKWoTwuW25QhZE6lyFzqnVYsM9PS23igPz_mR6fs01lxNhy4NUNZ5cYndo7aBhPPyPcwzvD4KpRl71c_0jg1Kt6uDiM0bsJOjpnCdAI7-wfHJ1_GlIvm3bi0DKNQygvJ-y6bFBP9vcX3iwYdeDT2GFmuRaWuef-_EOffhZPXItHhXbgzQEjyoZf5Pbjh6vtwqx8q-esB_J6NPZj7J5YkeJIV5CQsMc9Hti4MmccyvEZ13ThbRz6jhAiiVzJzq3O1DK36uW5IWMaajQV5c7WqkOXu7TuiyCx0RTHx3_P-eh5J-njRoXnUo4dwenjwdXaUDrMWUsPKrE01VbmZeseNpFx4WxoEVsJYq5Hv6AeM8bnhmlNLERJxKa0VXnjM1gSCNK_pI5jUoXZPgCgjtERXYaymzNpMC2do6SSVhdYIdxJ4veF1tepbalSYikShVFtCSWA_SmL8KHbC7hbC5bdqMKwKM0CuaVl4hchOxfZrLpeofBxxiXFaJ7C7kWM1mGdTXSlTAi_HbTSseFuiahfW-A0iH1EIQfMEHvdiHymJoFiWjCUgthRii9TtnXpx3jXvpiwiRv70_2Q9g9uIzPK-mG0XJu3l2j1H9NPqF4OK_wFkUgco
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Journals: Open Access
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrZ3Lb9QwEIdHUITEBfEmUJCREAKJQBM7dnxACFZUBbGIAyv1FvlJF22TspuV2jt_ODPJbkqgR655yZnxON_Yk58BnjrhgjE8poXIfSoQmVOtspDGYI3I3F4ZPU3oT7_Ig5n4dFgcnksKbQy4ujC1o_2kZsvFq9OfZ28x4N9Qxokp--v5j-MVDsUUtrK4DFdygUk6VfGJYUEBuaHbN41mPFIaoXu5zX9vH32eOhX_i9Dz7wrKPz5J-zfg-oYl2bve-TfhUqhvwdV-d8mz2_BrMogx9_9asiayrGBfmwUm_GjfuWNTqsdbmU6Wsw3sM7qKIcaySTg5MoumNafrFWsWVLwxZ8_Pjxq0fXjxkhk2abrqGHr2tF-nxyZ9PO4sih3qDsz2P3ybHKSbTRdSJ8qsTS03uduLQTrNpYq-dEhYynlvi1zigOBczJ20knuObCS19l5FFTFtU0hr0fK7sFM3dbgPzDhlNY4ZzlsuvM-sCo6XQXNdWIvck8Czra2rk15bo8KchJxSjZySwHvyxHARSWJ3B5rl92oTYRWmgtLysogGEc-QDlvINfZCiYDigrUJ7G79WG27WYX0IulfY5El8GQ4jRFGyyamDs0ar0EEUoVSPE_gXu_2oSVEx7oUIgE16hCjpo7P1POjTsWbC0JH-eB_vNtDuIYgl_e1b7uw0y7X4RHCUmsfd2HwG6nzFZw
  priority: 102
  providerName: Scholars Portal
Title Characterization of 15 polymorphic microsatellite loci for Cephalotaxus oliveri (Cephalotaxaceae), a conifer of medicinal importance
URI https://www.ncbi.nlm.nih.gov/pubmed/23109844
https://www.proquest.com/docview/1526022041
https://search.proquest.com/docview/1124757732
https://pubmed.ncbi.nlm.nih.gov/PMC3472736
https://doaj.org/article/2616b385fa644a1798e29a3f6521cebb
Volume 13
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ1Lb9QwEICttgiJCyrv0BIZCSGQSLeJHTs50qhLQaRaISrtLfKzDdpNVt2sBHd-OGMnWVjEiUsOzkOWZ8bzjTMeI_RKUWWEIDZKaaIjCsgc5Tw2kTVS0FidZla7Bf3ykl1c0U_zdL6H0nEvjE_aV7I-aRbLk6a-8bmVq6WajHlik1lZEOq8Lpvso31Q0DFEH6IsAkDfF9MkEM9P6m_LNczTzqaZO6bG8UyeUbrjh3y5_n8x5t-pkn_4nukhuj9AI37fd-4B2jPNQ3S3P0byxyP0s9hWXe43VeLW4jjFs3YBkT0MZK1w6RLv1sLX3-wM_gwywcCruDCrG7FoO_F9s8btwmVp1PjN71YBg2zevsMCF61Pg3HfLvsf8tClj0vP76A5j9HV9PxrcRENpytEimZxF0kiEnVqDVM5YdzqTAFKcaW1TBMGlq-UTRSTjGgCEMTyXGtuuYX4jAOWWUmeoIOmbcwzhIXiMofJQWlJqNax5EaRzOQkT6UEwAnQ63Gsq1VfRKOC4MPJp9qRT4DOnCS2D7na176hvb2uBg2oIOZjkmSpFcBywhVcM0kO6saARJSRMkDHoxyrwSDXFWAKc5uKaRygl9vbYEru_4hoTLuBZ4B1eMo5SQL0tBf7tiej2gSI7yjETld374D2-nLdg7Y-_-83j9A9wLSkz2w7Rgfd7ca8ABTqZAgGMOdwzaYfQnTn7Pxy9iX0ywqhc1EpXEuahd5AfgEluxMe
link.rule.ids 230,315,730,783,787,867,888,2109,2228,12068,12777,21400,24330,27936,27937,31731,31732,33385,33386,33756,33757,43322,43612,43817,53804,53806,74073,74363,74630
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3da9RAEB-0Ivoi9Tva6goiCoY22c1u9kns4XnVu-JDC30L-2lPrsnZ5EDf_cM7m-TSnoivuyEM8_mb3dkZgNeGGacU9XHGUhszhMyxFImLvdOKJWY_9zYc6M-O-OSEfTnNTvsDt7ovq1z7xNZR28qEM_I9jDM8vAplyYflzzhMjQq3q_0IjZtwi1GM1eGl-PjzkHDRtB2WlmAMinkmeddjk2Kavzf_cV6j-w6mHuLKtZjUtu7_F978u2zyWhwab8O9HkCSj53E78MNVz6A291Iyd8P4c9o6MDcPbAklSdJRr5VC8zykalzQ2ahCK9WbS_OxpEpyocgdiUjtzxTi6pRv1Y1qRahYmNO3l6tKmS4e_eeKDKq2pKY8O9ZdzmPJB2et1getegRnIw_HY8mcT9pITYsT5pYU5Wafe-4kZQLb3ODsEoYazVyHb2AMT41XHNqKQIiLqW1wguPuZpAiOY1fQxbZVW6p0CUEVqiozBWU2ZtooUzNHeSykxrBDsRvFnzulh2DTUKTESCUIoNoURwECQxfBT6YLcL1cX3ojerAvM_rmmeeYW4ToXmay6VqHocUYlxWkews5Zj0RtnXVypUgSvhm00q3BXokpXrfAbxD0iE4KmETzpxD5QEiCxzBmLQGwoxAapmzvl_Kxt3U1ZwIv82f_Jegl3JsezaTE9PPr6HO4iRku7srYd2GouVm4XcVCjX7TKfgmF0wiz
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwEB5BEYgL4tkGWjASQiARbRM7dnxCZWFpoVv1QKXeIj_bRdtkabIS3PnhjJNs2kWIqx1Fluf1jf15BuCVYcYpRX2csdTGDCFzLEXiYu-0YonZzb0NB_rTI75_wr6cZqc9_6nuaZUrn9g6aluZcEY-wjjDw6tQlox8T4s4_jh5v_gRhw5S4aa1b6dxE25hVORB5_PJ5yH5omnbOC3BeBTzTPKu3ibFlH80-35RoysPZh9izLX41Jbx_xf2_JtCeS0mTe7DvR5Mkr1O-g_ghisfwu2uveSvR_B7PFRj7h5bksqTJCPH1RwzftzgmSHTQMirVVuXs3HkEGVFEMeSsVucq3nVqJ_LmlTzwN6YkTdXowo33719RxQZVy09Jvx72l3U45IOLlpcjxr1GE4mn76N9-O-60JsWJ40saYqNbvecSMpF97mBiGWMNZqlAB6BGN8arjm1FIER1xKa4UXHvM2gXDNa_oENsqqdFtAlBFaotMwVlNmbaKFMzR3kspMawQ-Ebxe7XWx6IprFJiUBKEUa0KJ4EOQxPBRqIndDlSXZ0VvYgXmglzTPPMKMZ4KhdhcKlENOSIU47SOYHslx6I31Lq4UqsIXg7TaGLh3kSVrlriN4iBRCYETSPY7MQ-rCTAY5kzFoFYU4i1pa7PlLPztow3ZQE78qf_X9YLuIN6XhweHH19BncRrqUdw20bNprLpdtBSNTo562u_wHdngzr
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Characterization+of+15+Polymorphic+Microsatellite+Loci+for+Cephalotaxus+oliveri+%28Cephalotaxaceae%29%2C+a+Conifer+of+Medicinal+Importance&rft.jtitle=International+journal+of+molecular+sciences&rft.au=Yingchun+Miao&rft.au=Xuedong+Lang&rft.au=Shuaifeng+Li&rft.au=Jianrong+Su&rft.date=2012-09-01&rft.pub=MDPI+AG&rft.issn=1422-0067&rft.volume=13&rft.issue=9&rft.spage=11165&rft.epage=11172&rft_id=info:doi/10.3390%2Fijms130911165&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_2616b385fa644a1798e29a3f6521cebb
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1422-0067&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1422-0067&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1422-0067&client=summon