Detection of Japanese Encephalitis Virus RNA in Host-Questing Ticks in Japan, 2019–2020
Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine ( Sus scrofa domesticus ), and wading birds. We report here a...
Saved in:
| Published in | The American journal of tropical medicine and hygiene Vol. 106; no. 6; pp. 1725 - 1728 |
|---|---|
| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Institute of Tropical Medicine
01.06.2022
The American Society of Tropical Medicine and Hygiene |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among
Culex tritaeniorhynchus
mosquitoes, domestic swine (
Sus scrofa domesticus
), and wading birds. We report here an anomalous result that mosquito-borne JEV was detected in unfed host-questing ticks collected from the field in Japan. JEV genomic RNA was detected in four pools of
Haemaphysalis flava
nymphs collected in November and December 2019, and March 2020, when
Cx. tritaeniorhynchus
adults were not presumed to be active. Moreover, JEV antigenomic RNA was detected in some JEV-positive tick samples, suggesting virus replication in ticks. However, taken together with no infectious virus isolated, the possibility that the antigenomic RNA was derived from the undigested bloodmeal source in ticks cannot be ruled out. Thus, the role of the ticks as a natural reservoir for JEV remains to be confirmed. |
|---|---|
| AbstractList | Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine (Sus scrofa domesticus), and wading birds. We report here an anomalous result that mosquito-borne JEV was detected in unfed host-questing ticks collected from the field in Japan. JEV genomic RNA was detected in four pools of Haemaphysalis flava nymphs collected in November and December 2019, and March 2020, when Cx. tritaeniorhynchus adults were not presumed to be active. Moreover, JEV antigenomic RNA was detected in some JEV-positive tick samples, suggesting virus replication in ticks. However, taken together with no infectious virus isolated, the possibility that the antigenomic RNA was derived from the undigested bloodmeal source in ticks cannot be ruled out. Thus, the role of the ticks as a natural reservoir for JEV remains to be confirmed. Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine ( Sus scrofa domesticus ), and wading birds. We report here an anomalous result that mosquito-borne JEV was detected in unfed host-questing ticks collected from the field in Japan. JEV genomic RNA was detected in four pools of Haemaphysalis flava nymphs collected in November and December 2019, and March 2020, when Cx. tritaeniorhynchus adults were not presumed to be active. Moreover, JEV antigenomic RNA was detected in some JEV-positive tick samples, suggesting virus replication in ticks. However, taken together with no infectious virus isolated, the possibility that the antigenomic RNA was derived from the undigested bloodmeal source in ticks cannot be ruled out. Thus, the role of the ticks as a natural reservoir for JEV remains to be confirmed. Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine (Sus scrofa domesticus), and wading birds. We report here an anomalous result that mosquito-borne JEV was detected in unfed host-questing ticks collected from the field in Japan. JEV genomic RNA was detected in four pools of Haemaphysalis flava nymphs collected in November and December 2019, and March 2020, when Cx. tritaeniorhynchus adults were not presumed to be active. Moreover, JEV antigenomic RNA was detected in some JEV-positive tick samples, suggesting virus replication in ticks. However, taken together with no infectious virus isolated, the possibility that the antigenomic RNA was derived from the undigested bloodmeal source in ticks cannot be ruled out. Thus, the role of the ticks as a natural reservoir for JEV remains to be confirmed.Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a primary transmission cycle among Culex tritaeniorhynchus mosquitoes, domestic swine (Sus scrofa domesticus), and wading birds. We report here an anomalous result that mosquito-borne JEV was detected in unfed host-questing ticks collected from the field in Japan. JEV genomic RNA was detected in four pools of Haemaphysalis flava nymphs collected in November and December 2019, and March 2020, when Cx. tritaeniorhynchus adults were not presumed to be active. Moreover, JEV antigenomic RNA was detected in some JEV-positive tick samples, suggesting virus replication in ticks. However, taken together with no infectious virus isolated, the possibility that the antigenomic RNA was derived from the undigested bloodmeal source in ticks cannot be ruled out. Thus, the role of the ticks as a natural reservoir for JEV remains to be confirmed. |
| Author | Kimura, Toshiya Faizah, Astri Nur Azerigyik, Faustus Akankperiwen Hayashi, Toshihiko Higa, Yukiko Kobayashi, Daisuke Isawa, Haruhiko Kuwata, Ryusei Sawabe, Kyoko |
| Author_xml | – sequence: 1 givenname: Daisuke surname: Kobayashi fullname: Kobayashi, Daisuke organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 2 givenname: Ryusei surname: Kuwata fullname: Kuwata, Ryusei organization: Faculty of Veterinary Medicine, Okayama University of Science, Ehime, Japan – sequence: 3 givenname: Toshiya surname: Kimura fullname: Kimura, Toshiya organization: Meat Inspection Center of Ehime Prefecture, Ehime, Japan – sequence: 4 givenname: Astri Nur surname: Faizah fullname: Faizah, Astri Nur organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 5 givenname: Faustus Akankperiwen surname: Azerigyik fullname: Azerigyik, Faustus Akankperiwen organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 6 givenname: Yukiko surname: Higa fullname: Higa, Yukiko organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 7 givenname: Toshihiko surname: Hayashi fullname: Hayashi, Toshihiko organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 8 givenname: Kyoko surname: Sawabe fullname: Sawabe, Kyoko organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan – sequence: 9 givenname: Haruhiko surname: Isawa fullname: Isawa, Haruhiko organization: Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/35405649$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kU9rFTEUxYO02Nfq0q0E3Lhw6s2_yWQjlFptS1GUKrgKmUymL895yXOSEdz5HfyGfhIzr3_QgqvAze8e7jlnH-2EGBxCTwgcclqrl2aV18tDSiqQAA_QgnBZV6TmYgctAIBWqmZyD-2ntAIgDSXkIdpjgoOouVqgL69ddjb7GHDs8bnZmOCSwyfBus3SDD77hD_7cUr447sj7AM-jSlXHyaXsg9X-NLbr2kebzdfYApE_f75iwKFR2i3N0Nyj2_eA_Tpzcnl8Wl18f7t2fHRRWV5Q3JlWN-RrmlbTnhLewEMFO2ltI1RjLpadbIH1zHVSCKMc9ASaABsBwVva8MO0Ktr3c3Url1nXcijGfRm9Gsz_tDReP3vT_BLfRW_a0VBKcqLwPMbgTF-m43ptU_WDUOJIk5J0xKUUIxs0Wf30FWcxlDsFUo2gjAhRaGe_n3R3Sm3qRegugbsGFMaXX-HENBzq3rbqqZEz60Wnt3jrc9mLq0Y8sN_tv4AZtmlzw |
| CitedBy_id | crossref_primary_10_7883_yoken_JJID_2024_250 crossref_primary_10_1016_j_parint_2023_102834 crossref_primary_10_3390_v14122640 |
| Cites_doi | 10.7601/mez.62.109 10.3201/eid2601.190235 10.1128/JVI.00040-11 10.1016/j.virusres.2020.198254 10.3201/eid1004.030517 10.1093/jmedent/41.5.93 10.1017/S0950268806007710 10.3347/kjp.2017.55.2.185 10.1111/j.1755-0998.2008.02469.x 10.7601/mez.62.53 10.1128/CMR.18.4.608-637.2005 10.1056/NEJMc1701600 10.1007/BF01200415 10.3201/eid1501.080311 10.4269/ajtmh.1987.37.69S 10.1007/s00705-015-2488-x |
| ContentType | Journal Article |
| Copyright | Copyright Institute of Tropical Medicine Jun 2022 The American Society of Tropical Medicine and Hygiene 2022 |
| Copyright_xml | – notice: Copyright Institute of Tropical Medicine Jun 2022 – notice: The American Society of Tropical Medicine and Hygiene 2022 |
| DBID | AAYXX CITATION NPM 7X8 5PM |
| DOI | 10.4269/ajtmh.21-0700 |
| DatabaseName | CrossRef PubMed MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef PubMed MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic CrossRef |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1476-1645 |
| EndPage | 1728 |
| ExternalDocumentID | PMC9209924 35405649 10_4269_ajtmh_21_0700 |
| Genre | Journal Article |
| GeographicLocations | Japan |
| GeographicLocations_xml | – name: Japan |
| GroupedDBID | --- 23M 2WC 34G 36B 53G 5GY 5RE 5VS 6J9 AAYXX ABCQX ABPPZ ACGFO ADBBV ADTPD AENEX AIAGR ALMA_UNASSIGNED_HOLDINGS AOIJS BAWUL BTFSW CITATION DIK E3Z EBD EBS EJD EMB EMOBN F5P GX1 H13 HYE K-O KQ8 L7B MV1 OK1 P2P PQQKQ RHI RPM SV3 TR2 TST UPT W8F WH7 WOQ WOW ~KM NPM 7X8 5PM |
| ID | FETCH-LOGICAL-c481t-a3fd1d8bb414b2f503092f77c8a932e69d7f0ed398715aee0b10800cd04b2b6a3 |
| ISSN | 0002-9637 1476-1645 |
| IngestDate | Thu Aug 21 18:37:00 EDT 2025 Fri Jul 11 16:12:36 EDT 2025 Mon Jun 30 06:10:11 EDT 2025 Thu Apr 03 07:10:50 EDT 2025 Tue Jul 01 03:04:18 EDT 2025 Thu Apr 24 23:13:05 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c481t-a3fd1d8bb414b2f503092f77c8a932e69d7f0ed398715aee0b10800cd04b2b6a3 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Financial support: This work was supported by grants-in-aid for the Research Program on Emerging and Re-emerging Infectious Diseases from Japan Agency for Medical Research and development (AMED) Grant Number JP20fk0108067, Regulatory Science Research from Ministry of Health, Labor and Welfare Grant Number 20KC1001, and JSPS KAKENHI Grant Numbers JP18H02856 and JP20K15671. Authors’ addresses: Daisuke Kobayashi, Astri Nur Faizah, Faustus Akankperiwen Azerigyik, Yukiko Higa, Toshihiko Hayashi, Kyoko Sawabe, and Haruhiko Isawa, Department of Medical Entomology, National Institute of Infectious Diseases, Tokyo, Japan, E-mails: dkoba@nih.go.jp, astri.faizah@mail.u-tokyo.ac.jp, faustus@niid.go.jp, saperoi@niid.go.jp, thaya@nih.go.jp, sawabe@niid.go.jp, and hisawa@nih.go.jp. Ryusei Kuwata, Faculty of Veterinary Medicine, Okayama University of Science, Imabari City, Ehime, Japan, E-mail: r-kuwata@vet.ous.ac.jp. Toshiya Kimura, Meat Inspection Center of Ehime Prefecture, Oozu City, Ehime, Japan, E-mail: kimura-toshiya@pref.ehime.lg.jp. |
| OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/9209924 |
| PMID | 35405649 |
| PQID | 2678513575 |
| PQPubID | 105381 |
| PageCount | 4 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_9209924 proquest_miscellaneous_2649593124 proquest_journals_2678513575 pubmed_primary_35405649 crossref_primary_10_4269_ajtmh_21_0700 crossref_citationtrail_10_4269_ajtmh_21_0700 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2022-06-01 |
| PublicationDateYYYYMMDD | 2022-06-01 |
| PublicationDate_xml | – month: 06 year: 2022 text: 2022-06-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Deerfield, Illinois |
| PublicationTitle | The American journal of tropical medicine and hygiene |
| PublicationTitleAlternate | Am J Trop Med Hyg |
| PublicationYear | 2022 |
| Publisher | Institute of Tropical Medicine The American Society of Tropical Medicine and Hygiene |
| Publisher_xml | – name: Institute of Tropical Medicine – name: The American Society of Tropical Medicine and Hygiene |
| References | (b1) 2009; 15 (b3) 2012; 17 (b14) 1987; 37 Platonov (b4) 2012; 17 (b12) 2007; 44 (b17) 2011; 62 (b16) 2007; 135 (b13) 2004; 10 (b18) 2011; 62 (b8) 2020; 26 (b6) 2017 (b19) 2017; 55 (b20) 1987; 3 (b7) 2021; 292 (b15) 2019 (b2) 2017; 376 (b11) 2005; 18 (b10) 2009; 9 (b5) 2015; 160 (b9) 2011; 85 |
| References_xml | – year: 2017 ident: b6 – volume: 62: start-page: 109 year: 2011 ident: b17 article-title: Field collections of mosquito larvae in 2009 and 2010 in Ehime Prefecture, western Japan doi: 10.7601/mez.62.109 – volume: 26: start-page: 125 year: 2020 ident: b8 article-title: Distribution of Japanese encephalitis virus, Japan and Southeast Asia, 2016–2018 doi: 10.3201/eid2601.190235 – volume: 85: start-page: 6185 year: 2011 ident: b9 article-title: RNA splicing in a new rhabdovirus from Culex mosquitoes doi: 10.1128/JVI.00040-11 – volume: 292: start-page: 198254 year: 2021 ident: b7 article-title: A novel nyavirus lacking matrix and glycoprotein genes from Argas japonicus ticks doi: 10.1016/j.virusres.2020.198254 – volume: 10: start-page: 653 year: 2004 ident: b13 article-title: Ixodid and argasid tick species and West Nile virus doi: 10.3201/eid1004.030517 – volume: 44: start-page: 93 year: 2007 ident: b12 article-title: Host range specificity of flaviviruses: correlation with in vitro replication doi: 10.1093/jmedent/41.5.93 – volume: 17: start-page: 20221 year: 2012 ident: b3 article-title: Japanese encephalitis virus RNA detected in Culex pipiens mosquitoes in Italy – volume: 135: start-page: 974 year: 2007 ident: b16 article-title: Detection of antibodies to Japanese encephalitis virus in the wild boars in Hiroshima prefecture, Japan doi: 10.1017/S0950268806007710 – volume: 55: start-page: 185 year: 2017 ident: b19 article-title: Identification of tick species collected from wild boars and habitats of wild boars and domestic pigs in the Republic of Korea doi: 10.3347/kjp.2017.55.2.185 – volume: 9: start-page: 4 year: 2009 ident: b10 article-title: Molecular methods for arthropod bloodmeal identification and applications to ecological and vector‐borne disease studies doi: 10.1111/j.1755-0998.2008.02469.x – volume: 62: start-page: 53 year: 2011 ident: b18 article-title: Bloodmeal identification and feeding habits of mosquitoes (Diptera: Culicidae) collected at five islands in the Ryukyu Archipelago, Japan doi: 10.7601/mez.62.53 – volume: 18: start-page: 608 year: 2005 ident: b11 article-title: Biological transmission of arboviruses: reexamination of and new insights into components, mechanisms, and unique traits as well as their evolutionary trends doi: 10.1128/CMR.18.4.608-637.2005 – volume: 376: start-page: 1483 year: 2017 ident: b2 article-title: Autochthonous Japanese encephalitis with yellow fever coinfection in Africa doi: 10.1056/NEJMc1701600 – volume: 17: start-page: 20241 year: 2012 ident: b4 article-title: Does the Japanese encephalitis virus (JEV) represent a threat for human health in Europe? Detection of JEV RNA sequences in birds collected in Italy – volume: 3: start-page: 73 year: 1987 ident: b20 article-title: Effects of temperature on development and growth in the tick, Haemaphysalis longicornis doi: 10.1007/BF01200415 – volume: 15: start-page: 1 year: 2009 ident: b1 article-title: Past, present, and future of Japanese encephalitis doi: 10.3201/eid1501.080311 – volume: 37: start-page: 69S year: 1987 ident: b14 article-title: Overwintering mechanisms of mosquito-borne arboviruses in temperate climates doi: 10.4269/ajtmh.1987.37.69S – volume: 160: start-page: 2151 year: 2015 ident: b5 article-title: Isolation of Japanese encephalitis virus and a novel insect-specific flavivirus from mosquitoes collected in a cowshed in Japan doi: 10.1007/s00705-015-2488-x – start-page: 118 year: 2019 ident: b15 |
| SSID | ssj0018211 |
| Score | 2.3881521 |
| Snippet | Japanese encephalitis virus (JEV), a mosquito-borne virus, causes severe clinical symptoms in humans in the Asian-Pacific region, where it circulates in a... |
| SourceID | pubmedcentral proquest pubmed crossref |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 1725 |
| SubjectTerms | Arachnids Encephalitis Ribonucleic acid RNA Short Report Vectors (Biology) |
| Title | Detection of Japanese Encephalitis Virus RNA in Host-Questing Ticks in Japan, 2019–2020 |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/35405649 https://www.proquest.com/docview/2678513575 https://www.proquest.com/docview/2649593124 https://pubmed.ncbi.nlm.nih.gov/PMC9209924 |
| Volume | 106 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3db9MwELdgSNNeJr7pGMhIiJcuJU6cjz5WsKlsYg-og71FjuPQMJpUTSLU_fXcOYmbjk0CXqLWPieKf5fz3fnuTMhbCWtGwISwAi5di8fj0BJMKotzR8gkVQr-YLTFuT-94KeX3uUmhUBnl1TxSF7fmlfyP6hCG-CKWbL_gKy5KTTAb8AXroAwXP8K44-qUrJT-U5h2cPjJIfHmIg4R_06K4dfs1VdDr-cT9CxMS3KytIuTnQQzDJ5paNh9Uica1imx_Dqjt1XWWeb3JO8X2iiWhVLjXC3Pa_3Iebr7yAsDLecgbhY43lNTfx8VtZXm776l2h113VdqmwTDrCo9elHw1kBI9dm4TgR2bXQbqAJnjaCno6-0wLsXRNcNVKNoOWBb4Gp5t0mxjG9FteoH9ViPnKYBWLJ7tMBCsuFxhS9Vp7fFD29UTe767pPHjhgQmhz-9OZ2WEKwfJtaq7i095vPWuP7Hajt9WVP2yQm6G0Pd1k9pDst0YFnTQc8ojcU_ljsvu5xeUJ-WYYhRYp7RiF9hmFakahwCg0y-kWo1DNKNisRx5RwyZPycXJ8ezD1GpP1LAkD1llCTdNWBLGMWc8dlIP99ecNAhkKECPV_44CVJbJe4YzGhPKGXHGIJqy8QG8tgX7jOykxe5ekGoB2R-zLnC4tKODEOWCs4Fh6-bedJlA3LUTVwk23LzeOrJzwjMTpzySE955LAIp3xA3hnyZVNn5S7Cww6FqOX5MnJA5fKYC6bHgLwx3SAocfcLprSokYZjEW6QTQPyvAHNPKlDe0CCLTgNARZh3-7Js7kuxj7G3HOHH9x5z5dkb_MFHJKdalWrV6DIVvFrzZG_ATdunj4 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Detection+of+Japanese+Encephalitis+Virus+RNA+in+Host-Questing+Ticks+in+Japan%2C+2019-2020&rft.jtitle=The+American+journal+of+tropical+medicine+and+hygiene&rft.au=Kobayashi%2C+Daisuke&rft.au=Kuwata%2C+Ryusei&rft.au=Kimura%2C+Toshiya&rft.au=Faizah%2C+Astri+Nur&rft.date=2022-06-01&rft.eissn=1476-1645&rft_id=info:doi/10.4269%2Fajtmh.21-0700&rft_id=info%3Apmid%2F35405649&rft.externalDocID=35405649 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0002-9637&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0002-9637&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0002-9637&client=summon |