Dynamic nanoscale morphology of the ER surveyed by STED microscopy

• Published in: The Journal of cell biology Vol. 218; no. 1; pp. 83 - 96
• Main Authors: Schroeder, Lena K., Barentine, Andrew E.S., Merta, Holly, Schweighofer, Sarah, Zhang, Yongdeng, Baddeley, David, Bewersdorf, Joerg, Bahmanyar, Shirin
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 07.01.2019
• Subjects: Animals; Cells (biology); Cellular biology; Chlorocebus aethiops; COS Cells; Cytoskeleton; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Cytoskeleton - ultrastructure; Depletion; Emission analysis; Endoplasmic reticulum; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - metabolism; Endoplasmic Reticulum - ultrastructure; Gene Expression Regulation; Intracellular Membranes - drug effects; Intracellular Membranes - metabolism; Intracellular Membranes - ultrastructure; Light microscopy; Membrane structure; Membrane structures; Microscopy; Microscopy - methods; Microtubules - drug effects; Microtubules - metabolism; Microtubules - ultrastructure; Molecular Imaging - methods; Morphology; Nocodazole - pharmacology; Nogo Proteins - genetics; Nogo Proteins - metabolism; Nuclear Pore Complex Proteins - genetics; Nuclear Pore Complex Proteins - metabolism; Quantitative analysis; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; Sheets; Stimulated emission; Time-Lapse Imaging - statistics & numerical data; Tubules; Tubulin Modulators - pharmacology

The endoplasmic reticulum (ER) is composed of interconnected membrane sheets and tubules. Superresolution microscopy recently revealed densely packed, rapidly moving ER tubules mistaken for sheets by conventional light microscopy, highlighting the importance of revisiting classical views of ER struc...