Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system

• Published in: Journal of Asia-Pacific entomology Vol. 23; no. 1; pp. 268 - 273
• Main Authors: Kobayashi, Masahiko, Xu, Jian, Kakino, Kohei, Masuda, Akitsu, Hino, Masato, Fujimoto, Naoki, Minamihata, Kosuke, Kamiya, Noriho, Mon, Hiroaki, Iida, Hiroshi, Takahashi, Masateru, Kusakabe, Takahiro, Man Lee, Jae
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.04.2020; 한국응용곤충학회
• Subjects: Baculoviridae; Baculovirus; bioactive properties; Escherichia coli; genetic vectors; hosts; immune system; insect larvae; interleukin-4; medicine; MmIL-4; Mus musculus; proteins; Purification; screening; secretion; Silkworm expression system; silkworms; 농학; Purification; Baculovirus; MmIL-4; Silkworm expression system

[Display omitted] •Recombinant MmIL-4 was expressed and purified by the silkworm-BEVS.•C-tagged rMmIL-4 is a better construct to produce rMmIL-4 in the silkworm-BEVS.•We found that the n70 strain produced the highest amount of rMmIL-4 among 19 silkworm strains. Interleukine-4 (IL-4) is a cytokine th...