Leptin signaling impairs macrophage defenses against Salmonella Typhimurium
The dynamic interplay between metabolism and immune responses in health and disease, by which different immune cells impact on metabolic processes, are being increasingly appreciated. However, the potential of master regulators of metabolism to control innate immunity are less understood. Here, we s...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 33; pp. 16551 - 16560 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
13.08.2019
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Series | PNAS Plus |
Subjects | |
Online Access | Get full text |
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Summary: | The dynamic interplay between metabolism and immune responses in health and disease, by which different immune cells impact on metabolic processes, are being increasingly appreciated. However, the potential of master regulators of metabolism to control innate immunity are less understood. Here, we studied the cross-talk between leptin signaling and macrophage function in the context of bacterial infections. We found that upon infection with Gram-negative pathogens, such as Salmonella Typhimurium, leptin receptor (Lepr) expression increased in both mouse and human macrophages. Unexpectedly, both genetic Lepr ablation in macrophages and global pharmacologic leptin antagonization augmented lysosomal functions, reduced S. Typhimurium burden, and diminished inflammation in vitro and in vivo. Mechanistically, we show that leptin induction activates the mTORC2/Akt pathway and subsequently down-regulates Phlpp1 phosphatase, allowing for phosphorylated Akt to impair lysosomal-mediated pathogen clearance. These data highlight a link between leptin signaling, the mTORC2/Phlpp1/Akt axis, and lysosomal activity in macrophages and have important therapeutic implications for modulating innate immunity to combat Gram-negative bacterial infections. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Author contributions: J.F., G.P., and N.R. designed research; J.F., S.G., R.G., C.C., R.R., G.C., N.J.H., M.W., J.W.U.F., and N.R. performed research; V.T. and A.A. contributed new reagents/analytic tools; J.F., S.G., J.R., J.W.U.F., and N.R. analyzed data; and J.F., G.C., A.A., and N.R. wrote the paper. 1Present address: Department of Physiology, Institute of Neuroscience and Physiology, University of Gothenburg, SE-405 30 Gothenburg, Sweden. Edited by Andres Vazquez-Torres, University of Colorado School of Medicine, Aurora, CO, and accepted by Editorial Board Member Carl F. Nathan July 2, 2019 (received for review March 25, 2019) |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1904885116 |