Calcein Binding to Assess Mineralization in Hydrogel Microspheres

We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calc...

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Published inPolymers Vol. 13; no. 14; p. 2274
Main Authors White, Kristopher, Chalaby, Rabab, Lowe, Gina, Berlin, Jacob, Glackin, Carlotta, Olabisi, Ronke
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 11.07.2021
MDPI
Subjects
Alizarin
Biomedical materials
Bone marrow
calcein
Calcium
Calcium phosphates
Cell culture
Colorimetry
Communication
Fluorescence
hydrogel
Hydrogels
Hydroxyapatite
Microscopy
microsphere
Microspheres
Mineral deposits
Mineral oils
Mineralization
osteogenesis
Phosphatase
polymer
Stains & staining
United States > US
polymer
microsphere
mineralization
calcein
osteogenesis
hydrogel
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Abstract We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calcium also gives measures of mineralization, and in tissue cultures calcein and Alizarin Red S have been shown to bind to the same regions of mineral deposits. We show that when the mineralization takes place within hydrogel microspheres, Alizarin Red S does not stain mineral deposits as consistently as calcein. As tissue engineers increasingly encapsulate osteoprogenitors within hydrogel scaffolds, calcein staining may prove a more reliable method to assess this mineralization.
AbstractList We describe a method to assess mineralization by osteoblasts within microspheres using calcein. Fluorescence imaging of calcein bound to the calcium in hydroxyapatite permits assessment of the mineralized portion of the extracellular matrix. Colorimetric imaging of Alizarin Red S complexed with calcium also gives measures of mineralization, and in tissue cultures calcein and Alizarin Red S have been shown to bind to the same regions of mineral deposits. We show that when the mineralization takes place within hydrogel microspheres, Alizarin Red S does not stain mineral deposits as consistently as calcein. As tissue engineers increasingly encapsulate osteoprogenitors within hydrogel scaffolds, calcein staining may prove a more reliable method to assess this mineralization.
Author Berlin, Jacob
Chalaby, Rabab
White, Kristopher
Glackin, Carlotta
Lowe, Gina
Olabisi, Ronke
AuthorAffiliation 2 Department of Materials Engineering, University of Technology, Baghdad 10066, Iraq; rhhl2007@yahoo.com
4 Department of Molecular Medicine, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA; jacobberlincoh@gmail.com
3 Department of Neuroscience, Beckman Research Institute, City of Hope, Duarte, CA 91010, USA; glowe@coh.org (G.L.); cglackin@coh.org (C.G.)
1 Department of Biomedical Engineering, The Henry Samueli School of Engineering, University of California—Irvine, Irvine, CA 92697, USA; kris.white@uci.edu
AuthorAffiliation_xml – name: 1 Department of Biomedical Engineering, The Henry Samueli School of Engineering, University of California—Irvine, Irvine, CA 92697, USA; kris.white@uci.edu
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– name: 2 Department of Materials Engineering, University of Technology, Baghdad 10066, Iraq; rhhl2007@yahoo.com
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2021 by the authors. 2021
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Issue 14
Keywords polymer
microsphere
mineralization
calcein
osteogenesis
hydrogel
Language English
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SubjectTerms Alizarin
Biomedical materials
Bone marrow
calcein
Calcium
Calcium phosphates
Cell culture
Colorimetry
Communication
Fluorescence
hydrogel
Hydrogels
Hydroxyapatite
Microscopy
microsphere
Microspheres
Mineral deposits
Mineral oils
Mineralization
osteogenesis
Phosphatase
polymer
Stains & staining
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Title Calcein Binding to Assess Mineralization in Hydrogel Microspheres
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