Nek2 kinase displaces distal appendages from the mother centriole prior to mitosis

• Published in: The Journal of cell biology Vol. 219; no. 3; p. 1
• Main Authors: Viol, Linda, Hata, Shoji, Pastor-Peidro, Ana, Neuner, Annett, Murke, Florian, Wuchter, Patrick, Ho, Anthony D, Giebel, Bernd, Pereira, Gislene
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 02.03.2020
• Subjects: Animals; Appendages; Binding Sites; Cell cycle; Cell Cycle and Division; Cell division; Cell Line; Centrioles - enzymology; Centrioles - genetics; Centrosomes; Cilia; Cilia - enzymology; Cilia - genetics; Epithelial cells; Epithelial Cells - enzymology; Female; G2 Phase Cell Cycle Checkpoints; Hematopoietic Stem Cells - enzymology; Heredity; Humans; Kinases; Localization; Mammary gland; Mammary Glands, Animal - cytology; Mammary Glands, Animal - enzymology; Mice; Microtubule Proteins - genetics; Microtubule Proteins - metabolism; Mitosis; NIMA-Related Kinases - genetics; NIMA-Related Kinases - metabolism; Protein Binding; Retina; Retinal Pigment Epithelium - cytology; Retinal Pigment Epithelium - enzymology; Signal Transduction; Time Factors

Distal appendages (DAs) of the mother centriole are essential for the initial steps of ciliogenesis in G1/G0 phase of the cell cycle. DAs are released from centrosomes in mitosis by an undefined mechanism. Here, we show that specific DAs lose their centrosomal localization at the G2/M transition in...