Brugia malayi Thioredoxin Peroxidase as a Potential Vaccine Candidate Antigen for Lymphatic Filariasis

• Published in: Applied biochemistry and biotechnology Vol. 167; no. 5; pp. 1351 - 1364
• Main Authors: Anand, Setty Balakrishnan, Rajagopal, Vasudevan, Kaliraj, Perumal
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.07.2012; Springer Nature B.V
• Subjects: alum; animal models; Animals; Antibodies; Antibodies - blood; Antibodies - immunology; Antigens; Antigens - genetics; Antigens - immunology; Biochemistry; Biotechnology; blood; Brugia malayi; Brugia malayi - enzymology; Brugia malayi - genetics; Cell Proliferation; Chemistry; Chemistry and Materials Science; Cytokines; Cytokines - metabolism; cytology; Effectiveness; Elephantiasis, Filarial; Elephantiasis, Filarial - epidemiology; Elephantiasis, Filarial - immunology; Elephantiasis, Filarial - metabolism; Elephantiasis, Filarial - prevention & control; Endemic Diseases; Endemic Diseases - prevention & control; Enzymes; enzymology; epidemiology; Filariasis; genetics; Humans; Immune response; Immunity, Cellular; Immunity, Humoral; Immunization; Immunoglobulin G; immunology; interferon-gamma; Interleukin 5; Leukocytes (mononuclear); Lymphocytes T; Male; metabolism; Mice; mononuclear leukocytes; Parasitic diseases; Peripheral blood mononuclear cells; Peroxidase; Peroxiredoxins; Peroxiredoxins - genetics; Peroxiredoxins - immunology; prevention & control; Proteins; Spleen; Spleen - cytology; Spleen - immunology; T-Lymphocytes; T-Lymphocytes - immunology; Thioredoxin; Thioredoxin peroxidase; Vaccines; Vaccines - genetics; Vaccines - immunology; Vector-borne diseases; γ-Interferon; Thioredoxin peroxidase; Antigens; Vaccines; Filariasis

Attempts were made to evaluate the protective efficacy of Brugia malayi thioredoxin peroxidase (BmTPX) in a mouse model. Mice immunized with a protein vaccine containing rBmTPX developed higher titres (1:5,000/1:10,000) of anti-BmTPX antibodies, compared with the mice immunized with the alum control...