A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion

Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chi...

Full description

Saved in:
Bibliographic Details
Published inCells (Basel, Switzerland) Vol. 10; no. 11; p. 2855
Main Authors Pendharkar, Gaurav, Lu, Yen-Ta, Chang, Chia-Ming, Lu, Meng-Ping, Lu, Chung-Huan, Chen, Chih-Chen, Liu, Cheng-Hsien
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 22.10.2021
MDPI
Subjects
A549 Cells
Cancer
Cancer immunotherapy
Cell fusion
Cell Fusion - instrumentation
Cell hybrids
cell pairing
Cell Survival
Cell viability
Cytology
Design
dielectrophoresis
Efficiency
Electric fields
Electricity
Electrodes
Gravity
Humans
hydrodynamic trapping
Hydrodynamics
Imaging, Three-Dimensional
Immunotherapy
Microfluidics - instrumentation
Sedimentation
THP-1 Cells
Titanium
Trapping
United States > US
cell pairing
dielectrophoresis
hydrodynamic trapping
cell fusion
Online AccessGet full text

Cover

Abstract Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.
AbstractList Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.
Cancer cell-immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.Cancer cell-immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.
Author Pendharkar, Gaurav
Lu, Meng-Ping
Lu, Yen-Ta
Chen, Chih-Chen
Liu, Cheng-Hsien
Lu, Chung-Huan
Chang, Chia-Ming
AuthorAffiliation 4 Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30044, Taiwan
3 Department of Medical Research, MacKay Memorial Hospital, New Taipei City 10449, Taiwan; aming.chang@gmail.com (C.-M.C.); miriam01084@gmail.com (M.-P.L.)
1 Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, Taiwan; g.pendharkar@ieee.org (G.P.); woes6210@gmail.com (C.-H.L.); chihchen@mx.nthu.edu.tw (C.-C.C.)
2 Chest Department, MacKay Memorial Hospital, New Taipei City 10449, Taiwan; ytlhl@mmh.org.tw
AuthorAffiliation_xml – name: 1 Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, Taiwan; g.pendharkar@ieee.org (G.P.); woes6210@gmail.com (C.-H.L.); chihchen@mx.nthu.edu.tw (C.-C.C.)
– name: 4 Institute of Nanoengineering and Microsystems, National Tsing Hua University, Hsinchu 30044, Taiwan
– name: 2 Chest Department, MacKay Memorial Hospital, New Taipei City 10449, Taiwan; ytlhl@mmh.org.tw
– name: 3 Department of Medical Research, MacKay Memorial Hospital, New Taipei City 10449, Taiwan; aming.chang@gmail.com (C.-M.C.); miriam01084@gmail.com (M.-P.L.)
Author_xml – sequence: 1
  givenname: Gaurav
  orcidid: 0000-0002-8011-095X
  surname: Pendharkar
  fullname: Pendharkar, Gaurav
– sequence: 2
  givenname: Yen-Ta
  surname: Lu
  fullname: Lu, Yen-Ta
– sequence: 3
  givenname: Chia-Ming
  surname: Chang
  fullname: Chang, Chia-Ming
– sequence: 4
  givenname: Meng-Ping
  surname: Lu
  fullname: Lu, Meng-Ping
– sequence: 5
  givenname: Chung-Huan
  surname: Lu
  fullname: Lu, Chung-Huan
– sequence: 6
  givenname: Chih-Chen
  orcidid: 0000-0002-2195-4802
  surname: Chen
  fullname: Chen, Chih-Chen
– sequence: 7
  givenname: Cheng-Hsien
  orcidid: 0000-0003-1882-3892
  surname: Liu
  fullname: Liu, Cheng-Hsien
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34831078$$D View this record in MEDLINE/PubMed
BookMark eNptkk1r3DAQhkVJadI0x16LoZde3I4-7JUvhWC6SSClhe5d6GO80WJbrmwH9t9Hm90t2VBdJM088zLSO-_JWR96JOQjha-cV_DNYtuOFChlsijekAsGC54LAdXZi_M5uRrHDaQlaUmheEfOuZCcwkJekOk6--ltDE07e-dttmz9kNcPfsjq0Bnf-36d3W5dDG7b6y4Bq6iHYRfVvctuon70k5586HWb_UHnO-z396wJMatTf9lv7eOxYDmPKfeBvG10O-LVYb8kq-WPVX2b3_-6uauv73MrJJ1yWhg0BTrZlMa6gnFrQArUKLgrhDGyMow1UGjKSweVhtJIixbLyjhBkV-Su72sC3qjhug7HbcqaK-eAyGulY6Tty0qzSRwcAgMGrEQaFBajmXBGs2Lhuqk9X2vNcymQ2fTM6NuT0RPM71_UOvwqGRJSyirJPDlIBDD3xnHSXV-3PmnewzzqFgJAuhCcJbQz6_QTZhj-uFnigGrFnRHfXrZ0b9Wjt4mgO-BZO84RmyUPXiVGvStoqB2Q6ROhihV5a-qjsL_558AAWrK-A
CitedBy_id crossref_primary_10_1039_D3LC00887H
crossref_primary_10_3390_mi13122109
crossref_primary_10_1063_5_0205100
crossref_primary_10_1063_5_0250472
crossref_primary_10_1021_acs_chemrev_1c00677
crossref_primary_10_1016_j_snr_2025_100294
crossref_primary_10_1063_5_0108792
Cites_doi 10.1038/263696a0
10.1002/elps.201100082
10.1039/C7LC01131H
10.1126/science.1116447
10.1073/pnas.1313661111
10.1016/j.snb.2012.02.065
10.1007/s10404-008-0289-1
10.1088/0960-1317/19/1/015004
10.1002/elps.201100129
10.1016/j.snb.2012.12.034
10.1038/nm0597-558
10.1016/j.jim.2004.05.005
10.1023/B:BMMD.0000042050.95246.af
10.1007/s10404-011-0829-y
10.1038/s41598-018-21993-8
10.2217/imt.11.4
10.1063/1.5028158
10.1063/1.3422544
10.1038/srep22036
10.1089/153623003321512111
10.1063/1.3630125
10.1038/nmeth.1290
10.1049/iet-nbt:20080008
10.1038/nprot.2009.234
10.1038/nature10673
10.1007/s10544-010-9432-3
10.1126/sciadv.aat7828
10.1002/elps.201100579
10.1021/acs.analchem.0c00408
10.1083/jcb.89.3.674
10.1158/1078-0432.CCR-05-1330
10.1007/978-0-387-37754-4
10.1038/256495a0
10.1002/elps.201100227
10.1517/14712598.5.5.703
10.1038/s41467-019-11157-1
10.1109/28.31255
10.1073/pnas.0606625104
10.1109/TNB.2009.2035252
ContentType Journal Article
Copyright 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2021 by the authors. 2021
Copyright_xml – notice: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2021 by the authors. 2021
DBID AAYXX
CITATION
CGR
CUY
CVF
ECM
EIF
NPM
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
RC3
7X8
5PM
DOA
DOI 10.3390/cells10112855
DatabaseName CrossRef
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Journals
ProQuest Central (Alumni)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
ProQuest One Community College
ProQuest Central Korea
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
Biological Sciences
ProQuest Biological Science
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
Genetics Abstracts
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ : directory of open access journals
DatabaseTitle CrossRef
MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central
ProQuest One Applied & Life Sciences
Genetics Abstracts
Natural Science Collection
ProQuest Central Korea
Biological Science Collection
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Biotechnology and BioEngineering Abstracts
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
ProQuest One Academic (New)
MEDLINE - Academic
DatabaseTitleList

MEDLINE
MEDLINE - Academic
CrossRef
Publicly Available Content Database
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 4
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Biology
EISSN 2073-4409
ExternalDocumentID oai_doaj_org_article_a28030de020f474ebe8c3e652fa35f1a
PMC8616069
34831078
10_3390_cells10112855
Genre Research Support, Non-U.S. Gov't
Journal Article
GeographicLocations United States--US
GeographicLocations_xml – name: United States--US
GrantInformation_xml – fundername: Ministry of Science and Technology, Taiwan
  grantid: MOST 108-2221-E-007-078
– fundername: NTHU/MMH collaboration funds
  grantid: MMH-TH-10303
– fundername: NTHU/MMH collaboration funds
  grantid: NTHU-103N2765E1
– fundername: Ministry of Science and Technology, Taiwan
  grantid: MOST 106-2221-E-007-043-MY3
GroupedDBID 53G
5VS
8FE
8FH
AADQD
AAFWJ
AAYXX
ABDBF
ACUHS
ADBBV
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BAWUL
BBNVY
BCNDV
BENPR
BHPHI
CCPQU
CITATION
DIK
EBD
ESX
GROUPED_DOAJ
HCIFZ
HYE
IAO
IHR
ITC
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
CGR
CUY
CVF
ECM
EIF
NPM
8FD
ABUWG
AZQEC
DWQXO
FR3
GNUQQ
P64
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
RC3
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c481t-15beb5ed8f6bcd523cb084eae43d54bb89b22f05a136d09a06b8cece69bd41e3
IEDL.DBID M48
ISSN 2073-4409
IngestDate Wed Aug 27 01:25:32 EDT 2025
Thu Aug 21 18:27:03 EDT 2025
Fri Jul 11 00:46:37 EDT 2025
Fri Jul 25 12:00:12 EDT 2025
Thu Jan 02 22:57:09 EST 2025
Tue Jul 01 01:00:24 EDT 2025
Thu Apr 24 23:12:56 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 11
Keywords cell pairing
dielectrophoresis
hydrodynamic trapping
cell fusion
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c481t-15beb5ed8f6bcd523cb084eae43d54bb89b22f05a136d09a06b8cece69bd41e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors equally contributed to this work.
ORCID 0000-0002-8011-095X
0000-0002-2195-4802
0000-0003-1882-3892
OpenAccessLink http://journals.scholarsportal.info/openUrl.xqy?doi=10.3390/cells10112855
PMID 34831078
PQID 2602029712
PQPubID 2032536
ParticipantIDs doaj_primary_oai_doaj_org_article_a28030de020f474ebe8c3e652fa35f1a
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8616069
proquest_miscellaneous_2604017432
proquest_journals_2602029712
pubmed_primary_34831078
crossref_citationtrail_10_3390_cells10112855
crossref_primary_10_3390_cells10112855
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20211022
PublicationDateYYYYMMDD 2021-10-22
PublicationDate_xml – month: 10
  year: 2021
  text: 20211022
  day: 22
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Cells (Basel, Switzerland)
PublicationTitleAlternate Cells
PublicationYear 2021
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Hu (ref_26) 2011; 32
Tresset (ref_18) 2004; 6
Hsiao (ref_35) 2018; 12
Yang (ref_36) 2016; 6
ref_11
Koido (ref_3) 2005; 11
Clow (ref_17) 2010; 12
Dessain (ref_16) 2004; 291
Schoeman (ref_34) 2018; 8
Liu (ref_4) 2019; 10
Masuda (ref_31) 1989; 25
Rosenblatt (ref_6) 2005; 5
Milstein (ref_15) 1975; 256
Hu (ref_25) 2013; 178
Gel (ref_33) 2009; 8
Gong (ref_2) 1997; 3
Qin (ref_38) 2010; 5
White (ref_12) 1981; 89
Kimura (ref_20) 2011; 32
Zhang (ref_23) 2014; 111
Hu (ref_21) 2011; 5
Zakai (ref_13) 1976; 263
Skelley (ref_22) 2009; 6
Qu (ref_30) 2011; 11
Mellman (ref_5) 2011; 480
Oback (ref_10) 2003; 5
Zhao (ref_24) 2020; 92
Cowan (ref_9) 2005; 309
Tomita (ref_8) 2011; 3
Ching (ref_14) 2012; 166–167
Cao (ref_28) 2008; 5
Gast (ref_1) 2018; 4
Hu (ref_29) 2012; 33
Gel (ref_19) 2010; 4
Kemna (ref_7) 2011; 32
Ju (ref_27) 2008; 19
Techaumnat (ref_32) 2008; 2
Tan (ref_39) 2007; 104
Huang (ref_37) 2018; 18
References_xml – volume: 263
  start-page: 696
  year: 1976
  ident: ref_13
  article-title: Fusion of human erythrocyte ghosts promoted by the combined action of calcium and phosphate ions
  publication-title: Nature
  doi: 10.1038/263696a0
– volume: 32
  start-page: 2488
  year: 2011
  ident: ref_26
  article-title: A high-throughput dielectrophoresis-based cell electrofusion microfluidic device
  publication-title: Electrophoresis
  doi: 10.1002/elps.201100082
– volume: 18
  start-page: 1113
  year: 2018
  ident: ref_37
  article-title: Cell pairing and polyethylene glycol (PEG)-mediated cell fusion using two-step centrifugation-assisted single-cell trapping (CAScT)
  publication-title: Lab. A Chip.
  doi: 10.1039/C7LC01131H
– volume: 309
  start-page: 1369
  year: 2005
  ident: ref_9
  article-title: Nuclear Reprogramming of Somatic Cells After Fusion with Human Embryonic Stem Cells
  publication-title: Science
  doi: 10.1126/science.1116447
– volume: 111
  start-page: 2948
  year: 2014
  ident: ref_23
  article-title: Block-Cell-Printing for live single-cell printing
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1313661111
– volume: 166–167
  start-page: 292
  year: 2012
  ident: ref_14
  article-title: A circuit design of a low-cost, portable and programmable electroporation device for biomedical applications
  publication-title: Sens. Actuators Chem.
  doi: 10.1016/j.snb.2012.02.065
– volume: 5
  start-page: 669
  year: 2008
  ident: ref_28
  article-title: Study of high-throughput cell electrofusion in a microelectrode-array chip
  publication-title: Microfluid. Nanofluid.
  doi: 10.1007/s10404-008-0289-1
– volume: 19
  start-page: 015004
  year: 2008
  ident: ref_27
  article-title: An electrofusion chip with a cell delivery system driven by surface tension
  publication-title: J. Micromech. Microeng.
  doi: 10.1088/0960-1317/19/1/015004
– volume: 32
  start-page: 2496
  year: 2011
  ident: ref_20
  article-title: Dielectrophoresis-assisted massively parallel cell pairing and fusion based on field constriction created by a micro-orifice array sheet
  publication-title: Electrophoresis
  doi: 10.1002/elps.201100129
– volume: 178
  start-page: 63
  year: 2013
  ident: ref_25
  article-title: Cell electrofusion in microfluidic devices: A review
  publication-title: Sens. Actuators B Chem.
  doi: 10.1016/j.snb.2012.12.034
– volume: 3
  start-page: 558
  year: 1997
  ident: ref_2
  article-title: Induction of antitumor activity by immunization with fusions of dendritic and carcinoma cells
  publication-title: Nat. Med.
  doi: 10.1038/nm0597-558
– volume: 291
  start-page: 109
  year: 2004
  ident: ref_16
  article-title: High efficiency creation of human monoclonal antibody-producing hybridomas
  publication-title: J. Immunol. Methods
  doi: 10.1016/j.jim.2004.05.005
– volume: 6
  start-page: 213
  year: 2004
  ident: ref_18
  article-title: A Microfluidic Device for Electrofusion of Biological Vesicles
  publication-title: Biomed Microdev.
  doi: 10.1023/B:BMMD.0000042050.95246.af
– volume: 11
  start-page: 633
  year: 2011
  ident: ref_30
  article-title: Somatic and stem cell pairing and fusion using a microfluidic array device
  publication-title: Microfluid. Nanofluid.
  doi: 10.1007/s10404-011-0829-y
– volume: 8
  start-page: 3714
  year: 2018
  ident: ref_34
  article-title: Electrofusion of single cells in picoliter droplets
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-018-21993-8
– volume: 3
  start-page: 371
  year: 2011
  ident: ref_8
  article-title: Hybridoma technologies for antibody production
  publication-title: Immunotherapy
  doi: 10.2217/imt.11.4
– volume: 12
  start-page: 034108
  year: 2018
  ident: ref_35
  article-title: Automatic cell fusion via optically-induced dielectrophoresis and optically-induced locally-enhanced electric field on a microfluidic chip
  publication-title: Biomicrofluidics
  doi: 10.1063/1.5028158
– volume: 4
  start-page: 022808
  year: 2010
  ident: ref_19
  article-title: Dielectrophoretic cell trapping and parallel one-to-one fusion based on field constriction created by a micro-orifice array
  publication-title: Biomicrofluidics
  doi: 10.1063/1.3422544
– volume: 6
  start-page: 22036
  year: 2016
  ident: ref_36
  article-title: Optically-Induced Cell Fusion on Cell Pairing Microstructures
  publication-title: Sci. Rep.
  doi: 10.1038/srep22036
– volume: 5
  start-page: 3
  year: 2003
  ident: ref_10
  article-title: Cloned Cattle Derived from a Novel Zona-Free Embryo Reconstruction System
  publication-title: Cloning Stem Cells
  doi: 10.1089/153623003321512111
– volume: 5
  start-page: 034121
  year: 2011
  ident: ref_21
  article-title: A cell electrofusion microfluidic device integrated with 3D thin-film microelectrode arrays
  publication-title: Biomicrofluidics
  doi: 10.1063/1.3630125
– volume: 6
  start-page: 147
  year: 2009
  ident: ref_22
  article-title: Microfluidic control of cell pairing and fusion
  publication-title: Nat. Methods
  doi: 10.1038/nmeth.1290
– volume: 2
  start-page: 93
  year: 2008
  ident: ref_32
  article-title: High-yield electrofusion of biological cells based on field tailoring by microfabricated structures
  publication-title: IET Nanobiotechnol.
  doi: 10.1049/iet-nbt:20080008
– volume: 5
  start-page: 491
  year: 2010
  ident: ref_38
  article-title: Soft lithography for micro- and nanoscale patterning
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2009.234
– volume: 480
  start-page: 480
  year: 2011
  ident: ref_5
  article-title: Cancer immunotherapy comes of age
  publication-title: Nature
  doi: 10.1038/nature10673
– volume: 12
  start-page: 777
  year: 2010
  ident: ref_17
  article-title: A novel micropit device integrates automated cell positioning by dielectrophoresis and nuclear transfer by electrofusion
  publication-title: Biomed. Microdev.
  doi: 10.1007/s10544-010-9432-3
– volume: 4
  start-page: eaat7828
  year: 2018
  ident: ref_1
  article-title: Cell fusion potentiates tumor heterogeneity and reveals circulating hybrid cells that correlate with stage and survival
  publication-title: Sci. Adv.
  doi: 10.1126/sciadv.aat7828
– volume: 33
  start-page: 1980
  year: 2012
  ident: ref_29
  article-title: A cell electrofusion microfluidic chip using discrete coplanar vertical sidewall microelectrodes
  publication-title: Electrophoresis
  doi: 10.1002/elps.201100579
– volume: 92
  start-page: 7638
  year: 2020
  ident: ref_24
  article-title: Microfluidic Control of Tumor and Stromal Cell Spheroids Pairing and Merging for Three-Dimensional Metastasis Study
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.0c00408
– volume: 89
  start-page: 674
  year: 1981
  ident: ref_12
  article-title: Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses
  publication-title: J. Cell Biol.
  doi: 10.1083/jcb.89.3.674
– volume: 11
  start-page: 7891
  year: 2005
  ident: ref_3
  article-title: Dendritic Cells Fused with Allogeneic Colorectal Cancer Cell Line Present Multiple Colorectal Cancer–Specific Antigens and Induce Antitumor Immunity against Autologous Tumor Cells
  publication-title: Clin. Cancer Res.
  doi: 10.1158/1078-0432.CCR-05-1330
– ident: ref_11
  doi: 10.1007/978-0-387-37754-4
– volume: 256
  start-page: 495
  year: 1975
  ident: ref_15
  article-title: Continuous cultures of fused cells secreting antibody of predefined specificity
  publication-title: Nature
  doi: 10.1038/256495a0
– volume: 32
  start-page: 3138
  year: 2011
  ident: ref_7
  article-title: On chip electrofusion of single human B cells and mouse myeloma cells for efficient hybridoma generation
  publication-title: Electrophoresis
  doi: 10.1002/elps.201100227
– volume: 5
  start-page: 703
  year: 2005
  ident: ref_6
  article-title: Dendritic cell fusion vaccines for cancer immunotherapy
  publication-title: Expert Opin. Biol. Ther.
  doi: 10.1517/14712598.5.5.703
– volume: 10
  start-page: 3199
  year: 2019
  ident: ref_4
  article-title: Cytomembrane nanovaccines show therapeutic effects by mimicking tumor cells and antigen presenting cells
  publication-title: Nat. Commun.
  doi: 10.1038/s41467-019-11157-1
– volume: 25
  start-page: 732
  year: 1989
  ident: ref_31
  article-title: Novel method of cell fusion in field constriction area in fluid integration circuit
  publication-title: IEEE Trans. Ind. Appl.
  doi: 10.1109/28.31255
– volume: 104
  start-page: 1146
  year: 2007
  ident: ref_39
  article-title: A trap-and-release integrated microfluidic system for dynamic microarray applications
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0606625104
– volume: 8
  start-page: 300
  year: 2009
  ident: ref_33
  article-title: Microorifice-Based High-Yield Cell Fusion on Microfluidic Chip: Electrofusion of Selected Pairs and Fusant Viability
  publication-title: IEEE Trans. NanoBiosci.
  doi: 10.1109/TNB.2009.2035252
SSID ssj0000816105
Score 2.2227764
Snippet Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips...
Cancer cell-immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 2855
SubjectTerms A549 Cells
Cancer
Cancer immunotherapy
Cell fusion
Cell Fusion - instrumentation
Cell hybrids
cell pairing
Cell Survival
Cell viability
Cytology
Design
dielectrophoresis
Efficiency
Electric fields
Electricity
Electrodes
Gravity
Humans
hydrodynamic trapping
Hydrodynamics
Imaging, Three-Dimensional
Immunotherapy
Microfluidics - instrumentation
Sedimentation
THP-1 Cells
Titanium
Trapping
SummonAdditionalLinks – databaseName: DOAJ : directory of open access journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1La9wwEBYhUMiltEnaOo-iQuipJtbLlo_p0u1SSAg0hdyMRo_GsHGWzW4h_74j2bvslpZecrXG6DEjzYwYfR8hZ9q40uta5pVzIZdGhNxIJ3LQ1hUKPLgEsXF5VU5-yG-36naD6ivWhPXwwP3CnZtIn1Q4j2FNkJXEPrUVvlQ8GKECS6ER-ryNZCqdwRojmUL1oJoC8_rzeA_-iPaH53F81rfhhBJW_98CzD_rJDccz_gVeTlEjPSiH-lrsuO7ffKi55B8OiCLC3oZi-rCdNm61tLxtJ3lo7t2RnGrQ6J_oJMnh-dkzz1P0TtFTIaf1HSOfp2bXwNKN_bxHT3Z_fAYqaMYztIRzodem3a--mG8jNdrh-Rm_OVmNMkHKoXcSs0WOYvLrrzToQTrMPm0UGjpjZfCKQmga-A8FMowUbqiNkWJyvLWlzU4ybx4Q3a7h86_I5TXDADzacMBpFEA3pma6SpYlK0qkZFPq6Vt7DCByHYxbTDdiJpotjSRkY9r8VmPr_Evwc9RT2uhCIudPqCxNIOxNP8zloycrLTcDHv1scGMjkcKL8Yz8mHdjLss9m86_7BMMpiIYrSFMm97o1iPRMhI1lbpjFRb5rI11O2Wrr1LSN66ZJhA1kfPMbdjssdjvQ36Vc5PyO5ivvSnGDAt4H3aG78BVZQYpg
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1La9wwEBZtQqGX0HfdpkGF0lNFbEm25VNJlmyXQkJoU8jN6JkYtt7NPgr5952xtW62NL1aYyRrZjQPj-Yj5IPSrvCqkqx0LjCpRWBaOsGMsi7NjTeua7FxelZMfsivl_llTLgtY1nl5kzsDmo3s5gjPwS_myPQUsY_z28Yokbh39UIofGQ7MIRrCD42j0-OTv_NmRZEFYCPIi-uaaA-P4Q8-FLkEMgx-t9d4xR17P_X47m3_WSdwzQ-AnZi54jPepZ_ZQ88O0z8qjHkrx9TlZH9BSL68J03bjG0vG0mbPRdTOnoPKmg4Ggk1sH52WPQU_BSmFvhiuqW0e_LPSv2K0b5vgOFu1nvJTUUnBr6Qi-h57rZrF5YbzGNNsLcjE-uRhNWIRUYFaqbMUy3P7cOxUKYx0EodakSnrtpXC5NEZVhvOQ5joThUsrnRbANG99URknMy9ekp121vrXhPIqMwbias2NkTo3xjtdZaoMFmjLUiTk02Zraxs_AFEvpjWEHciJeosTCfk4kM_7Phv3ER4jnwYibI_dPZgtruqobbVGzK3UeRCZIEsJgqqs8EXOgxZ5yHRC9jdcrqPOLus_EpaQ98MwaBvOr1s_W3c0EJCC1wU0r3qhGFYiJIK2lSoh5Za4bC11e6RtrruO3qrIIJCs3vx_WW_JY44VNWA5Od8nO6vF2r8Dl2hlDqLc_wZM6BEf
  priority: 102
  providerName: ProQuest
Title A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
URI https://www.ncbi.nlm.nih.gov/pubmed/34831078
https://www.proquest.com/docview/2602029712
https://www.proquest.com/docview/2604017432
https://pubmed.ncbi.nlm.nih.gov/PMC8616069
https://doaj.org/article/a28030de020f474ebe8c3e652fa35f1a
Volume 10
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB5BK1AviDeBdmUkxIlA4kfiHKqqXXVZIW1VQSv1Ftmx3UZasku6i9h_zziPpVvKhWsyVhLPjGc-y_k-gHdSmcTKjIepMS7kirlQccNCLQsTCW21aSg2JifJ-Jx_uRAXfyiFugm8vhPaeT2p83r68deP1QEm_L5HnAjZP_kt7msMLVxqhbgP21iUUp-jk67TbxZlia1NJFqWzb9H7cBDxr3mlpdbu1GgGh7_u5rP22cobxSl0WN41HWT5LB1_xO4Z6un8KDVl1w9g8UhmfgDd266LE1ZkNG0nIfDq3JOcBnQjTQEGa8MrqGtLj3ByuX5Gi6Jqgz5XKufHYM3PuMbVrnv3Y9KFcFWlwzx08ipKut-wGjpt96ew9no-Gw4DjuZhbDgMl6EsXeJsEa6RBcGgWmhI8mtspwZwbWWmabURULFLDFRpqIEHWkLm2Ta8NiyF7BVzSr7CgjNYq0RayuqNVdCa2tUFsvUFWibpiyAD_3U5kX3AV4JY5ojFPFOyTecEsD7tfm85d74l-GR99PayFNmNxdm9WXeZWCuvA5XZCz2x46nHINXFswmgjrFhItVALu9l_M-DHNEe9TLe8U0gLfr25iB_vmqsrNlY4MgFTsxtHnZBsX6TfqgCiDdCJeNV928U5VXDcu3TGIEl9nr_x75BnaoP4CDhZbSXdha1Eu7hx3UQg9g--j45PTroNmBGDSZ8huf5SK-
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEB6VVAguFW9SCiwScMKqvbu21weE2tCQ0iaqIEi9Wftyayk4IQ9QfhT_kVm_aBBw69U79o497_XufAAvhTSRFQn3YmMyj0uWeZIb5imhjR8qq0zZYmM4igZf-Mfz8HwLfjZnYdy2ysYnlo7aTLVbI9_HvJs6oKWAvpt98xxqlPu72kBoVGpxYtc_sGRbvD1-j_J9RWn_aNwbeDWqgKe5CJZe4DgIrRFZpLTBOkwrX3ArLWcm5EqJRFGa-aEMWGT8RPoR8m21jRJleGAZPvYGbHOGlUwHtg-PRmef2kUdh2KBCUvVy5OxxN93y-8LVHsMA-404ZXYV0IE_C2v_XN75pV4178DO3WiSg4qzboLW7a4Bzcr6Mr1fVgekKHby5dNVrnJNelP8pnXu8xnBD2MKlEnyGBt0D1XkPcEg6JrBXFBZGHIh7n8XjcHxzk-YwD9Wp-BKghm0aSH70POZD5vbuiv3KreAxhfx7d-CJ1iWtjHQGgSKIVlvKRKcRkqZY1MAhFnGmnjmHXhTfNpU12_gAPZmKRY5ThJpBuS6MLrlnxWtfX4F-Ghk1NL5Lpxlxem84u0Nu5UOogv31jU0IzHHO1CaGajkGaShVkgu7DXSDmtXcQi_a3QXXjRDqNxu_llYaerkgbrX0zykOZRpRQtJ4w7jLhYdCHeUJcNVjdHivyybCAuogDr1mT3_2w9h1uD8fA0PT0enTyB29Rt5sGgTekedJbzlX2K2dhSPattgEB6zVb3C5Q1T3w
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwEB6VrUBcEM8SKGAk4ES0ie0kzgGhdtuwpXS1giL1Ftmx00baZpd9gPan8e8Y50UXAbdek0lie97OeD6AV0Lq0IiYu5HWucsly13JNXOVyLQXKKN01WLjZBQOv_KPZ8HZFvxsz8LYssrWJlaGWk8zu0fex7ibWqAln_bzpixifJC8n31zLYKU_dPawmnUInJs1j8wfVu8OzpAXr-mNDk8HQzdBmHAzbjwl65vRxMYLfJQZRpzskx5ghtpONMBV0rEitLcC6TPQu3F0gtxDiYzYaw09w3D196A7QiTIq8H2_uHo_HnboPHIlpg8FL39WQs9vp2K36BKoAuwZ4svOIHK7iAv8W4f5ZqXvF9yV240wStZK-WsnuwZcr7cLOGsVw_gOUeObF1fflkVegiI8mkmLmDi2JG0NqoCoGCDNcaTfW6lJdIgA7StoU4J7LU5MNcfm8aheM3vqAzvWzOQ5UEI2oywPmQsSzm7QPJyu7wPYTT61jrR9Arp6V5DITGvlKY0kuqFJeBUkbL2BdRniFtFDEH3rZLm2bNBCzgxiTFjMdyIt3ghANvOvJZ3eLjX4T7lk8dke3MXV2Yzs_TRtFTaeG-PG1QWnMecdQRkTETBjSXLMh96cBuy-W0MReL9LdwO_Cyu42Kbr8vSzNdVTSYC2PAhzQ7tVB0I2Hc4sVFwoFoQ1w2hrp5pywuqmbiIvQxh42f_H9YL-AWalv66Wh0_BRuU1vXg_6b0l3oLecr8wwDs6V63qgAgfSale4XyXNTsQ
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+Microfluidic+Flip-Chip+Combining+Hydrodynamic+Trapping+and+Gravitational+Sedimentation+for+Cell+Pairing+and+Fusion&rft.jtitle=Cells+%28Basel%2C+Switzerland%29&rft.au=Pendharkar%2C+Gaurav&rft.au=Lu%2C+Yen-Ta&rft.au=Chang%2C+Chia-Ming&rft.au=Lu%2C+Meng-Ping&rft.date=2021-10-22&rft.pub=MDPI&rft.eissn=2073-4409&rft.volume=10&rft.issue=11&rft_id=info:doi/10.3390%2Fcells10112855&rft_id=info%3Apmid%2F34831078&rft.externalDocID=PMC8616069
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2073-4409&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2073-4409&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2073-4409&client=summon