Bet v 1 and its homologous food allergen Api g 1 stimulate dendritic cells from birch pollen-allergic individuals to induce different Th-cell polarization

• Published in: Allergy (Copenhagen) Vol. 65; no. 11; pp. 1388 - 1396
• Main Authors: Smole, U, Wagner, S, Balazs, N, Radauer, C, Bublin, M, Allmaier, G, Hoffmann-Sommergruber, K, Breiteneder, H
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.11.2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Adult; Allergens; Allergens - immunology; Allergies; Antigens, Plant - immunology; Bet v 1 antigen; Betula - immunology; Biological and medical sciences; birch pollen allergy; birch pollen food syndrome; Cell culture; Cell Polarity; Cell proliferation; Cell surface; Cells, Cultured; Coculture Techniques; cross reaction; Cross Reactions; cross reactivity; Cytokines - biosynthesis; Dendritic cells; Dendritic Cells - immunology; Dermatology; Enzyme-Linked Immunosorbent Assay; Female; Flow cytometry; Food hypersensitivity; Food Hypersensitivity - immunology; Food plants; Fundamental and applied biological sciences. Psychology; Fundamental immunology; g-Interferon; gamma -Interferon; Granulocyte-macrophage colony-stimulating factor; Helper cells; Humans; Hypersensitivity; Immunoglobulin E; Interleukin 13; Interleukin 4; Interleukin 5; Lymphocyte Activation - immunology; Lymphocytes T; Mal d 1 antigen; Male; Medical sciences; Monocytes; Peripheral blood; Plant Proteins - immunology; Polarization; Pollen; Rhinitis, Allergic, Seasonal - immunology; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Th1 Cells - cytology; Th1 Cells - immunology; Th1/Th2; Th2 Cells - cytology; Th2 Cells - immunology; Trees; Allergy; Insecta; Apidae; Th1 lymphocyte; Homology; Immunology; dendritic cells; Antigen presenting cell; Apoidea; Dicotyledones; Angiospermae; T-Lymphocyte; Cross reaction; Th2 lymphocyte; Pollen; birch pollen allergy; Betulaceae; Aculeata; Food; Human; Th1/Th2; Dendritic cell; Immunopathology; Food allergen; Dermatology; Apis; birch pollen food syndrome; cross reactivity; Arthropoda; Spermatophyta; Hymenoptera; Invertebrata; Betula
• ISSN: 0105-4538; 1398-9995
• DOI: 10.1111/j.1398-9995.2010.02407.x

To cite this article: Smole U, Wagner S, Balazs N, Radauer C, Bublin M, Allmaier G, Hoffmann-Sommergruber K, Breiteneder H. Bet v 1 and its homologous food allergen Api g 1 stimulate dendritic cells from birch pollen-allergic individuals to induce different Th-cell polarization. Allergy 2010; 65: 1388-1396. Bet v 1 is the most relevant sensitizing protein for birch pollen (BP)-allergic individuals. Its homologues from plant foods are mainly involved in allergic reactions caused by IgE cross reactivity. We aimed to evaluate the polarizing effect of dendritic cells (DCs) pulsed with Bet v 1, Mal d 1, Api g 1 or Dau c 1 on Th-cell responses. Immature DCs were generated from peripheral blood monocytes of BP-allergic and healthy donors by culture with GM-CSF and IL-4 and subsequently pulsed with allergens in combination with maturation factors. Cell surface markers were analysed by FACS. Mature DCs were co-cultured with autologous Th cells and T-cell proliferation and cytokine profiles were determined. In co-culture, mature allergen-pulsed DCs induced autologous Th cells of BP-allergic donors to proliferate significantly more than those of healthy individuals. Exposure of DCs from BP-allergic donors to Bet v 1 resulted in a robust Th2 skewing with significantly higher quantities of IL-5 and elevated IL-13 compared to maturation factors. In contrast, Api g 1-primed DCs from BP allergics significantly enhanced the production of the Th1 cytokine IFN-γ and significantly down-regulated IL-13 compared to maturation factors. In healthy donors, no significant cytokine production could be detected. Bet v 1 in contrast to homologous food allergens seems to possess distinct molecular features that enable it to condition DCs from BP-allergic donors to induce allergen-specific T-cell proliferation and Th2 polarization.