Improved microscale cultivation of Pichia pastoris for clonal screening

• Published in: Fungal biology and biotechnology Vol. 5; no. 1; p. 8
• Main Authors: Eck, Alexander, Schmidt, Matthias, Hamer, Stefanie, Ruff, Anna Joelle, Förster, Jan, Schwaneberg, Ulrich, Blank, Lars M, Wiechert, Wolfgang, Oldiges, Marco
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.05.2018; BioMed Central; BMC
• Subjects: Analysis; Bioprocess development; Enzymes; Escherichia coli; Fed-batch; High throughput; Microbioreactor; Physiological aspects; Pichia pastoris; Screening; High throughput; Microbioreactor; Screening; Bioprocess development; Phytase; Fed-batch; Pichia pastoris

Expanding the application of technical enzymes, e.g., in industry and agriculture, commands the acceleration and cost-reduction of bioprocess development. Microplates and shake flasks are massively employed during screenings and early phases of bioprocess development, although major drawbacks such a...