A phage protein that binds φC31 integrase to switch its directionality

• Published in: Molecular microbiology Vol. 80; no. 6; pp. 1450 - 1463
• Main Authors: Khaleel, Thanafez, Younger, Ellen, McEwan, Andrew R., Varghese, Anpu S., Smith, Margaret C. M.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2011; Blackwell
• Subjects: Attachment Sites, Microbiological; Biological and medical sciences; Escherichia coli - virology; Fundamental and applied biological sciences. Psychology; Integrases - genetics; Integrases - metabolism; Microbiology; Miscellaneous; Molecular Sequence Data; Phage Bxb1; Protein Binding; Recombination, Genetic; Streptococcus Phages - enzymology; Streptococcus Phages - genetics; Streptococcus Phages - metabolism; Streptomyces; Viral Proteins - genetics; Viral Proteins - metabolism; Virology; Virus Integration; Virus; Protein; Phage

Summary The serine integrase, Int, from the Streptomyces phage φC31 mediates the integration and excision of the phage genome into and out of the host chromosome. Integrases usually require a recombination directionality factor (RDF) or Xis to control integration and excision and, as φC31 Int only m...