Micellar electrokinetic capillary chromatography for the determination of Viagra and its metabolite (UK-103,320) in human serum

• Published in: Electrophoresis Vol. 22; no. 10; pp. 2004 - 2009
• Main Authors: Nevado, Juan J. Berzas, Flores, Juana Rodríguez, Peñalvo, Gregorio Castañeda, Fariñas, Nuria Rodríguez
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.06.2001
• Subjects: Electrophoresis, Capillary - methods; Electrophoresis, Capillary - statistics & numerical data; Human serum; Humans; Male; Micellar electrokinetic capillary chromatography; Micelles; Osmolar Concentration; Phosphodiesterase Inhibitors - blood; Phosphodiesterase Inhibitors - metabolism; Piperazines - blood; Piperazines - metabolism; Purines; Pyrimidinones - blood; Pyrimidinones - metabolism; Sildenafil Citrate; Sildenafil citrate (Viagra); Sildenafil metabolite (UK-103; Sildenafil metabolite (UK‐103,320); Sodium Dodecyl Sulfate; Sulfones; Temperature

Micellar electrokinetic capillary chromatography (MEKC) was investigated for the determination of Viagra (sildenafil citrate, SC) and its metabolite (UK‐103,320) in human serum in a concentration range of clinical interest. For MEKC, human serum samples spiked with SC and UK were obtained directly a...