Interaction of coronavirus nucleocapsid protein with the 5′‐ and 3′‐ends of the coronavirus genome is involved in genome circularization and negative‐strand RNA synthesis

• Published in: The FEBS journal Vol. 286; no. 16; pp. 3222 - 3239
• Main Authors: Lo, Chen‐Yu, Tsai, Tsung‐Lin, Lin, Chao‐Nan, Lin, Ching‐Hung, Wu, Hung‐Yi
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.08.2019; John Wiley and Sons Inc
• Subjects: (−)‐strand synthesis; Affinity; Animals; binding capacity; Bovine coronavirus; Cattle; Circularity; cis‐acting element; Coronaviridae; coronavirus; Coronavirus, Bovine - genetics; Coronavirus, Bovine - growth & development; Coronaviruses; dissociation; genome; genome circularization; Genome, Viral - genetics; Genomes; N protein; nucleocapsid; nucleocapsid protein; nucleocapsid proteins; Nucleocapsid Proteins - genetics; Nucleocapsids; Nucleotides; Original; Poly A - genetics; Polyadenine; Polyadenylation; Polynucleotides; Proteins; regulatory sequences; Replication; RNA; RNA - biosynthesis; RNA - genetics; RNA, Messenger - genetics; RNA, Viral - genetics; RNA-binding protein; RNA-binding proteins; RNA-Binding Proteins - genetics; Synthesis; Transcription; Virus Replication - genetics; genome circularization; replication; coronavirus; nucleocapsid protein; (−)-strand synthesis; cis-acting element

Synthesis of the negative‐strand ((−)‐strand) counterpart is the first step of coronavirus (CoV) replication; however, the detailed mechanism of the early event and the factors involved remain to be determined. Here, using bovine coronavirus (BCoV)‐defective interfering (DI) RNA, we showed that (a)...