Microbial communities and nutrient dynamics in experimental microcosms are altered after the application of a high dose of Bti

• Published in: The Journal of applied ecology Vol. 52; no. 3; pp. 763 - 773
• Main Authors: Duguma, Dagne, Hall, Michael W., Rugman-Jones, Paul, Stouthamer, Richard, Neufeld, Josh D.
• Format: Journal Article
• Language: English
• Published: Oxford John Wiley & Sons Ltd 01.06.2015; Blackwell Publishing Ltd
• Subjects: application rate; aquatic food webs; Aquatic insects; Bacillus thuringiensis subsp. israelensis; Bacteria; bacteria communities; bacterial communities; biogeochemical cycles; biomass; biopesticide; Biopesticides; Bti; community interactions; Culex; Culex mosquitoes; Cyanobacteria; Cyclobacteriaceae; Disease and pest management; Food chains; Food webs; genes; Habitats; larvae; Microbial activity; Microcosms; mosquito control; Mosquitoes; Nutrient cycles; Nutrient dynamics; nutrients; outdoor aquatic microcosms; particulates; Pesticides; Phytoplankton; Polaromonas; Primary production; primary productivity; ribosomal RNA; Simuliidae; Sphingobacteriia; Sphingomonas; unintentional effects; vector control; Water column
• DOI: 10.1111/1365-2664.12422
• ISSN: 0021-8901

1. Bacillus thuringiensis subsp. israelensis (Bti) is the most widely used biopesticide against mosquitoes and blackflies, with a history of high specificity and efficacy. High doses of Bti have been suggested for extended vector control in some environments; however, the effects of Bti application on the native microfauna in the environment are poorly understood. 2. Two Bti (VectoBac G) treatments (high = 48·1 kg ha⁻¹; low = 0·6 kg ha⁻¹), in addition to an untreated control, were assigned to replicate 300 L microcosms. 3. Culex abundance, phytoplankton biomass, sestonic particulates and nutrients were reduced significantly in the high Bti treatment. These changes affected other physicochemical variables in the water column during the 44-day field study. 4. Bacterial communities present in the water column were assessed by Illumina sequencing of 16S rRNA genes. The most abundant aquatic bacteria in microcosms subject to a low dose of Bti and untreated control, Cyanobacteria, Cytophagales, Cyclobacteriaceae (phylum Bacteroidetes) and Sphingomonas (class Alphaproteobacteria), were suppressed in microcosms subject to a high dose of Bti. Bacteria in the high Bti treatment were dominated by Mucilaginibacter, Sediminibacterium (phylum Sphingobacterid) and Polaromonas (class Betaproteobacterid) were more diverse than in the other treatments. 5. Synthesis and applications. The biotic and abiotic changes resulting from a biopesticide application that significantly reduced mosquito abundance by more than 50% persisted longer than the period during which larval mosquito numbers were reduced. This warranted further investigation into the ecosystem-level effects of Bti application rates used routinely for mosquito control. Application rates greater than the label rate for a Bti biopesticide can reduce mosquito abundance for an extended period of time and therefore lessen the operational cost of repeated application, but they could exceed manufacturer's recommendations and might violate countryspecific regulations governing biopesticide applications in natural habitats. Results of our study suggest that the widespread adoption of Bti application above the recommended label rate should be discouraged in habitats where algal abundance and its effect on primary production, microbial communities and nutrient cycling could affect the functioning of aquatic food webs.