Senescent dermal fibroblasts negatively influence fibroblast extracellular matrix‐related gene expression partly via secretion of complement factor D

• Published in: BioFactors (Oxford) Vol. 45; no. 4; pp. 556 - 562
• Main Authors: Ezure, Tomonobu, Sugahara, Misato, Amano, Satoshi
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.07.2019
• Subjects: Cell Proliferation; Cellular Senescence - drug effects; CFD; Coculture Techniques; Collagen Type I - genetics; Collagen Type I - metabolism; Complement Factor D - antagonists & inhibitors; Complement Factor D - genetics; Complement Factor D - metabolism; Complement Factor D - pharmacology; Diffusion Chambers, Culture; Elastin - genetics; Elastin - metabolism; Extracellular Matrix - chemistry; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; fibroblast; Fibroblasts - cytology; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene Expression Regulation; Humans; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 1 - metabolism; MMP1; Primary Cell Culture; Research Communication; Research Communications; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; SASP; senescence; Signal Transduction; CFD; senescence; fibroblast; MMP1; SASP

Aging is associated with a decrease of extracellular matrix and an increase of senescent cells in the dermal layer. Here, to examine whether and how senescent cells are involved in aging‐related deterioration of the dermal layer, we cocultured dermal young fibroblasts (low‐passage number) with senes...