Improved Super-Resolution Microscopy with Oxazine Fluorophores in Heavy Water

Brighter dyes in heavy water: A simple and cost‐effective method increases the brightness of a whole class of commonly used red‐emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H2O) by heavy water (D2O) in the imaging buffer doubles the fluorescence quantum yield of t...

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Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 52; no. 34; pp. 8948 - 8951
Main Authors Lee, Steven F., Vérolet, Quentin, Fürstenberg, Alexandre
Format Journal Article
LanguageEnglish
Published Weinheim WILEY-VCH Verlag 19.08.2013
WILEY‐VCH Verlag
Wiley Subscription Services, Inc
EditionInternational ed. in English
Subjects
Buffers
Chemical compounds
Dyes
dyes/pigments
Fluorescence
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Heavy water
Imaging
Localization
Microscopy
Microscopy, Fluorescence
oxazines
Oxazines - chemical synthesis
Oxazines - chemistry
super-resolution microscopy
Water - chemistry
dyes/pigments
oxazines
fluorescence
super-resolution microscopy
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Summary:Brighter dyes in heavy water: A simple and cost‐effective method increases the brightness of a whole class of commonly used red‐emitting fluorophores, including ATTO655, ATTO680, and ATTO700. Replacing water (H2O) by heavy water (D2O) in the imaging buffer doubles the fluorescence quantum yield of these dyes and significantly improves the localization precision in super‐resolution imaging.
Bibliography:National Science Foundation - No. PZ00P3_131935
istex:C3B6A565C6897DA2E76A0FF784119374EA7423F9
ark:/67375/WNG-69C3B9DM-7
This project was supported by the Swiss National Science Foundation through Ambizione fellowship PZ00P3_131935 and by Dormeur Investment Service Ltd. We thank Oliver Hartley for lab space, support, and cell lines, Thomas Huber for helpful comments, and Eric Vauthey for useful discussions and for sharing equipment including the TCSPC detection system.
ArticleID:ANIE201302341
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201302341