Development and validation of an LC-MS/MS method for the quantification of flavonoid glucuronides (wogonoside, baicalin, and apigenin-glucuronide) in the bile and blood samples: Application to a portal vein infusion study

• Published in: Analytical biochemistry Vol. 601; p. 113723
• Main Authors: Tu, Yifan, Zhou, Lei, Li, Li, Wang, Lu, Gao, Song, Hu, Ming
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.07.2020
• Subjects: acetonitrile; ammonium acetate; Animals; Apigenin - analysis; Apigenin-7-O-Glucuronide; Baicalin; Bile; Bile - chemistry; biochemical pathways; Blood; blood sampling; chemical species; Chromatography, High Pressure Liquid; Flavanones - analysis; Flavonoids - analysis; Glucosides - analysis; Glucuronide quantification; high performance liquid chromatography; LC-MS/MS; Male; monitoring; portal vein; Portal Vein - chemistry; Rats; Rats, Wistar; solid phase extraction; SPE; spectrometers; Tandem Mass Spectrometry; taurocholic acid; Wogonoside; LC-MS/MS; Apigenin-7-O-Glucuronide; SPE; Glucuronide quantification; Wogonoside; Baicalin; Blood; Bile

Glucuronidation is one of the major metabolic pathways for flavonoids. However, quantification of flavonoid glucuronides in biological samples, especially in the bile, is sometimes challenging due to signal suppression by bile acids. The purpose of this study is to establish a robust LC-MS/MS method...