Molecular Determinants in tRNA D-arm Required for Inhibition of HIV-1 Gag Membrane Binding

• Published in: Journal of molecular biology Vol. 434; no. 2; p. 167390
• Main Authors: Sumner, Christopher, Kotani, Osamu, Liu, Shuohui, Musier-Forsyth, Karin, Sato, Hironori, Ono, Akira
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30.01.2022
• Subjects: acidic phospholipid; Anticodon - metabolism; Binding Sites; Cell Membrane - metabolism; gag Gene Products, Human Immunodeficiency Virus - chemistry; gag Gene Products, Human Immunodeficiency Virus - metabolism; highly basic region; HIV-1 - genetics; HIV-1 - metabolism; Humans; lipid-protein interaction; molecular biology; Molecular Docking Simulation; particles; Phosphatidylinositol 4,5-Diphosphate; Protein Interaction Domains and Motifs; RNA; RNA, Transfer - chemistry; RNA, Transfer - metabolism; RNA, Viral - genetics; testing; tRNA–protein interaction; virus assembly; Virus Assembly - physiology; acidic phospholipid; tRNA–protein interaction; virus assembly; highly basic region; lipid-protein interaction

[Display omitted] •Features of tRNA contributing to inhibition of HIV-1 Gag membrane binding are unclear.•Membrane binding of purified MA is strongly inhibited by tRNALys3 but not tRNAPro.•The D arm but not anticodon arm contributes to strong inhibition by tRNALys3.•The D arm sequence and its contex...