Quantification of cortisol and its metabolites in human urine by LC-MSn: applications in clinical diagnosis and anti-doping control

• Published in: Analytical and bioanalytical chemistry Vol. 414; no. 23; pp. 6841 - 6853
• Main Authors: Arioli, Francesco, Gamberini, Maria Cristina, Pavlovic, Radmila, Di Cesare, Federica, Draghi, Susanna, Bussei, Giulia, Mungiguerra, Francesca, Casati, Alessio, Fidani, Marco
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.09.2022; Springer Nature B.V
• Subjects: Analytical Chemistry; analytical methods; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Corticoids; Corticosteroids; Cortisol; Cortisone; Cushing syndrome; detection limit; Drug abuse; Food Science; Hormones; Human wastes; humans; Hypothalamus; Laboratory Medicine; Linearity; Liquid chromatography; liquids; Metabolism; Metabolites; Monitoring/Environmental Analysis; Negative feedback; Negative ions; Pituitary; Prednisolone; quantitative analysis; Research Paper; Spectrometry; Urine; Addison syndrome; Doping control; Linear ion trap mass spectrometry; Cortisol metabolites; Human urine; Cushing syndrome

The objective of the current research was to develop a liquid chromatography-MS n (LC-MS n ) methodology for the determination of free cortisol and its 15 endogenous metabolites (6β-hydroxycortisol, 20α-dihydrocortisol, 20α-dihydrocortisone, 20-β-dihydrocortisol, 20β-dihydrocortisone, prednisolone,...