The porphyrin TmPyP4 unfolds the extremely stable G-quadruplex in MT3-MMP mRNA and alleviates its repressive effect to enhance translation in eukaryotic cells

• Published in: Nucleic acids research Vol. 40; no. 9; pp. 4137 - 4145
• Main Authors: Morris, Mark J, Wingate, Katherine L, Silwal, Jagannath, Leeper, Thomas C, Basu, Soumitra
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.05.2012
• Subjects: C.D; DNA; G-Quadruplexes - drug effects; Gel electrophoresis; Genes, Reporter; HeLa Cells; Humans; Matrix Metalloproteinase 16 - genetics; N.M.R; Nuclear Magnetic Resonance, Biomolecular; Porphyrins; Porphyrins - chemistry; Porphyrins - pharmacology; Protein Biosynthesis; purines; Reporter gene; RNA; RNA, Messenger - chemistry; Spectroscopy; Translation

We report that the cationic porphyrin TmPyP4, which is known mainly as a DNA G-quadruplex stabilizer, unfolds an unusually stable all purine RNA G-quadruplex (M3Q) that is located in the 5'-UTR of MT3-MMP mRNA. When the interaction between TmPyP4 and M3Q was monitored by UV spectroscopy a 22-nm...