Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR

• Published in: British journal of cancer Vol. 123; no. 12; pp. 1790 - 1795
• Main Authors: Arroyo Mühr, Laila Sara, Lagheden, Camilla, Lei, Jiayao, Eklund, Carina, Nordqvist Kleppe, Sara, Sparén, Pär, Sundström, Karin, Dillner, Joakim
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 08.12.2020; Nature Publishing Group UK
• Subjects: Adult; Aged; Aged, 80 and over; Alphapapillomavirus - isolation & purification; Cervical cancer; Cervix; Confidence Intervals; DNA, Viral - isolation & purification; Female; High-Throughput Nucleotide Sequencing - methods; High-Throughput Nucleotide Sequencing - statistics & numerical data; Human papillomavirus; Humans; Invasiveness; Medical screening; Medicin och hälsovetenskap; Middle Aged; Negative Results - statistics & numerical data; Nucleotide sequence; Paraffin; Pilot projects; Polymerase chain reaction; Quality assurance; Reverse Transcriptase Polymerase Chain Reaction; Sweden; Tumors; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; Sweden

Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formal...