Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR
Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formal...
Saved in:
Published in | British journal of cancer Vol. 123; no. 12; pp. 1790 - 1795 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Nature Publishing Group
08.12.2020
Nature Publishing Group UK |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formalin-fixed paraffin-embedded samples.
Using Novaseq 6000, we simultaneously sequenced total DNA and cDNA from 392 HPV PCR-negative cervical cancers. Non-human reads were queried against all known HPVs. The complete database now contains PCR and/or deep sequencing data on 2850 invasive cervical cancers.
HPV sequences were detected in 169/392 of HPV PCR-negative cervical cancers. Overall, 30 different HPV types were detected, but only 5 types were present in proportions above 3% of cancers. More than 92% of tumours were HPV-positive in PCR and/or sequencing (95% confidence interval: 91.1-93.1%). Exploring possible reasons for failure to previously detect HPV suggest that more sensitive type-specific PCRs for HPV 31, 33, 45 and 73 targeting retained regions of HPV would have detected most of these (117/392).
Unbiased deep sequencing provides comprehensive data on HPV types in cervical cancers and appears to be an important tool for quality assurance of HPV screening. |
---|---|
AbstractList | BackgroundHuman papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formalin-fixed paraffin-embedded samples.MethodsUsing Novaseq 6000, we simultaneously sequenced total DNA and cDNA from 392 HPV PCR-negative cervical cancers. Non-human reads were queried against all known HPVs. The complete database now contains PCR and/or deep sequencing data on 2850 invasive cervical cancers.ResultsHPV sequences were detected in 169/392 of HPV PCR-negative cervical cancers. Overall, 30 different HPV types were detected, but only 5 types were present in proportions above 3% of cancers. More than 92% of tumours were HPV-positive in PCR and/or sequencing (95% confidence interval: 91.1–93.1%). Exploring possible reasons for failure to previously detect HPV suggest that more sensitive type-specific PCRs for HPV 31, 33, 45 and 73 targeting retained regions of HPV would have detected most of these (117/392).ConclusionsUnbiased deep sequencing provides comprehensive data on HPV types in cervical cancers and appears to be an important tool for quality assurance of HPV screening. Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formalin-fixed paraffin-embedded samples. Using Novaseq 6000, we simultaneously sequenced total DNA and cDNA from 392 HPV PCR-negative cervical cancers. Non-human reads were queried against all known HPVs. The complete database now contains PCR and/or deep sequencing data on 2850 invasive cervical cancers. HPV sequences were detected in 169/392 of HPV PCR-negative cervical cancers. Overall, 30 different HPV types were detected, but only 5 types were present in proportions above 3% of cancers. More than 92% of tumours were HPV-positive in PCR and/or sequencing (95% confidence interval: 91.1-93.1%). Exploring possible reasons for failure to previously detect HPV suggest that more sensitive type-specific PCRs for HPV 31, 33, 45 and 73 targeting retained regions of HPV would have detected most of these (117/392). Unbiased deep sequencing provides comprehensive data on HPV types in cervical cancers and appears to be an important tool for quality assurance of HPV screening. Abstract Background Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously requested all invasive cervical cancers in Sweden during 10 years and subjected them to PCR. We also optimised methods for deep sequencing of formalin-fixed paraffin-embedded samples. Methods Using Novaseq 6000, we simultaneously sequenced total DNA and cDNA from 392 HPV PCR-negative cervical cancers. Non-human reads were queried against all known HPVs. The complete database now contains PCR and/or deep sequencing data on 2850 invasive cervical cancers. Results HPV sequences were detected in 169/392 of HPV PCR-negative cervical cancers. Overall, 30 different HPV types were detected, but only 5 types were present in proportions above 3% of cancers. More than 92% of tumours were HPV-positive in PCR and/or sequencing (95% confidence interval: 91.1–93.1%). Exploring possible reasons for failure to previously detect HPV suggest that more sensitive type-specific PCRs for HPV 31, 33, 45 and 73 targeting retained regions of HPV would have detected most of these (117/392). Conclusions Unbiased deep sequencing provides comprehensive data on HPV types in cervical cancers and appears to be an important tool for quality assurance of HPV screening. |
Author | Lagheden, Camilla Nordqvist Kleppe, Sara Sundström, Karin Arroyo Mühr, Laila Sara Dillner, Joakim Eklund, Carina Lei, Jiayao Sparén, Pär |
Author_xml | – sequence: 1 givenname: Laila Sara orcidid: 0000-0003-2498-5206 surname: Arroyo Mühr fullname: Arroyo Mühr, Laila Sara email: sara.arroyo.muhr@ki.se organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden. sara.arroyo.muhr@ki.se – sequence: 2 givenname: Camilla surname: Lagheden fullname: Lagheden, Camilla organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden – sequence: 3 givenname: Jiayao orcidid: 0000-0002-4718-1414 surname: Lei fullname: Lei, Jiayao organization: Department of Medical Epidemiology & Biostatistics, Karolinska Institutet, Stockholm, Sweden – sequence: 4 givenname: Carina surname: Eklund fullname: Eklund, Carina organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden – sequence: 5 givenname: Sara surname: Nordqvist Kleppe fullname: Nordqvist Kleppe, Sara organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden – sequence: 6 givenname: Pär orcidid: 0000-0002-5184-8971 surname: Sparén fullname: Sparén, Pär organization: Department of Medical Epidemiology & Biostatistics, Karolinska Institutet, Stockholm, Sweden – sequence: 7 givenname: Karin orcidid: 0000-0002-6865-0224 surname: Sundström fullname: Sundström, Karin organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden – sequence: 8 givenname: Joakim orcidid: 0000-0001-8588-6506 surname: Dillner fullname: Dillner, Joakim organization: Center for Cervical Cancer Prevention, Department of Laboratory Medicine, Karolinska Institutet and Karolinska University Laboratory, Karolinska University Hospital, Stockholm, Sweden |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/33020595$$D View this record in MEDLINE/PubMed http://kipublications.ki.se/Default.aspx?queryparsed=id:144841364$$DView record from Swedish Publication Index |
BookMark | eNpdkV1vFCEUhompsdvqH_DCkHjTXozyOTA3Jmat1qSJjVEvJQxzZkudgRFm1vTfy7prYyUhHOB5z4HznqCjEAMg9JySV5Rw_ToLKmhdEUYqQsuoyCO0opKzimqmjtCKEKIq0jByjE5yvi3bhmj1BB1zXkSykSv0_R3AhDP8XCA4Hza4gxncnPHNMtqAJzv5YYij3fq0ZHx2ef3tHPuAHaStd3bAzoYSZxxgY2e_BdzHhAuF2zt8vf78FD3u7ZDh2WE9RV_fX3xZX1ZXnz58XL-9qpzQZK5qbpmWteaUtcB7a6VrOtYBAdVIIpQUNRc91c7JulUKGkp62ZJOS911rKb8FDX7vPkXTEtrpuRHm-5MtL7EsTOH8x9-N00GQ4XQgvJaFO2bvbYAI3QOwpzs8DDFg5vgb8wmbo1SjCnRlARnhwQplj7m2Yw-OxgGGyAu2bBSSotdqYK-_A-9jUsKpTWFUsUWqlhdKLanXIo5J-jvH0OJ2Vlv9tab4qL5Y70hRfTi32_cS_56zX8DdVWrVQ |
CitedBy_id | crossref_primary_10_1016_j_jcv_2024_105657 crossref_primary_10_1155_2021_6631757 crossref_primary_10_1038_s41598_022_17318_5 crossref_primary_10_1186_s13027_022_00420_8 crossref_primary_10_1038_s41598_022_14730_9 crossref_primary_10_1002_ijc_33769 crossref_primary_10_1158_2159_8290_CD_22_0489 crossref_primary_10_1200_JCO_21_01930 crossref_primary_10_1002_jmv_29332 crossref_primary_10_1002_ijc_33683 crossref_primary_10_3390_bioengineering11050468 crossref_primary_10_1097_AOG_0000000000005286 crossref_primary_10_1016_j_talanta_2024_126114 crossref_primary_10_1038_s41416_023_02555_w crossref_primary_10_3390_v13050739 crossref_primary_10_1002_ijc_33519 crossref_primary_10_1038_s41598_021_98452_4 crossref_primary_10_1016_j_virol_2021_03_002 crossref_primary_10_1038_s41416_023_02233_x crossref_primary_10_1038_s41598_022_19008_8 crossref_primary_10_3389_fonc_2023_1333812 crossref_primary_10_1080_14737159_2023_2173580 crossref_primary_10_1002_ijc_33590 crossref_primary_10_12998_wjcc_v12_i20_4146 crossref_primary_10_3390_v13071323 crossref_primary_10_1097_SCS_0000000000008113 crossref_primary_10_1371_journal_pone_0278117 crossref_primary_10_1097_PAS_0000000000001778 crossref_primary_10_1371_journal_pmed_1004304 crossref_primary_10_3390_ijms241813786 crossref_primary_10_1002_slct_202200655 crossref_primary_10_1016_j_arcmed_2022_11_009 crossref_primary_10_1158_1078_0432_CCR_23_1078 crossref_primary_10_1097_PAS_0000000000002067 crossref_primary_10_1136_ijgc_2021_003014 |
Cites_doi | 10.1038/nature21386 10.1038/nmeth.3176 10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F 10.1038/s41416-018-0053-6 10.1186/1743-422X-9-262 10.1099/jgv.0.001374 10.1002/ijc.29722 10.1371/journal.pone.0065953 10.1016/j.jcv.2013.07.013 10.1016/j.pvr.2017.10.003 10.1002/ijc.29325 10.1128/JCM.02007-08 10.1016/j.jcv.2018.01.016 10.1038/modpathol.2014.55 10.5858/arpa.2016-0356-RA 10.1016/j.jcv.2016.04.016 10.1002/ijc.31017 10.1002/ijc.29651 10.1093/bioinformatics/btu170 10.1371/journal.pmed.1002666 10.1002/ijc.25396 10.1016/j.virol.2015.07.023 10.1016/j.vaccine.2012.06.095 10.1093/bioinformatics/btt468 10.18632/oncotarget.14533 10.1016/j.jcv.2007.09.009 |
ContentType | Journal Article |
Copyright | The Author(s), under exclusive licence to Cancer Research UK 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. The Author(s), under exclusive licence to Cancer Research UK 2020 |
Copyright_xml | – notice: The Author(s), under exclusive licence to Cancer Research UK 2020. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: The Author(s), under exclusive licence to Cancer Research UK 2020 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION 3V. 7RV 7TO 7U9 7X7 7XB 88E 8AO 8C1 8FE 8FH 8FI 8FJ 8FK ABUWG AFKRA AN0 AZQEC BBNVY BENPR BHPHI CCPQU DWQXO FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB0 LK8 M0S M1P M7P NAPCQ PQEST PQQKQ PQUKI PRINS 7X8 5PM ADTPV AOWAS D8T ZZAVC |
DOI | 10.1038/s41416-020-01111-0 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef ProQuest Central (Corporate) ProQuest Nursing and Allied Health Journals Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts ProQuest Health & Medical Collection ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection ProQuest Public Health Database ProQuest SciTech Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central British Nursing Database ProQuest Central Essentials Biological Science Collection ProQuest Central Natural Science Collection ProQuest One Community College ProQuest Central Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection (Proquest) (PQ_SDU_P3) ProQuest Health & Medical Complete (Alumni) Nursing & Allied Health Database (Alumni Edition) Biological Sciences Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Biological Science Database Nursing & Allied Health Premium ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) SwePub SwePub Articles SWEPUB Freely available online SwePub Articles full text |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) SciTech Premium Collection ProQuest One Community College ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central China ProQuest Central Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Natural Science Collection ProQuest Central Korea Biological Science Collection AIDS and Cancer Research Abstracts ProQuest Medical Library (Alumni) ProQuest Public Health Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition British Nursing Index with Full Text ProQuest Nursing & Allied Health Source ProQuest Hospital Collection Health Research Premium Collection (Alumni) Biological Science Database ProQuest SciTech Collection ProQuest Hospital Collection (Alumni) Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest Medical Library ProQuest One Academic UKI Edition ProQuest Nursing & Allied Health Source (Alumni) ProQuest One Academic ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | ProQuest Central Student MEDLINE CrossRef MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Medicine |
EISSN | 1532-1827 |
EndPage | 1795 |
ExternalDocumentID | oai_prod_swepub_kib_ki_se_144841364 10_1038_s41416_020_01111_0 33020595 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GeographicLocations | Sweden |
GeographicLocations_xml | – name: Sweden |
GrantInformation_xml | – fundername: Stiftelsen för Strategisk Forskning (Swedish Foundation for Strategic Research) grantid: RB13-0011 – fundername: ; – fundername: ; grantid: RB13-0011 |
GroupedDBID | --- -Q- .55 .GJ 0R~ 23N 36B 39C 3V. 4.4 406 53G 5GY 5RE 6J9 70F 7RV 7X7 88E 8AO 8C1 8FI 8FJ 8R4 8R5 8WZ A6W AACDK AANZL AASML AATNV AAWBL AAWTL AAZLF ABAKF ABAWZ ABDBF ABLJU ABOCM ABUWG ABZZP ACAOD ACGFO ACGFS ACKTT ACPRK ACRQY ACZOJ ADBBV ADFRT ADHDB AEFQL AEJRE AEMSY AENEX AEVLU AEXYK AFBBN AFKRA AFRAH AFSHS AGAYW AGEZK AGHAI AGQEE AHMBA AHSBF AI. AIGIU AILAN AJRNO ALFFA ALMA_UNASSIGNED_HOLDINGS AMYLF AN0 AOIJS ASPBG AVWKF AXYYD AZFZN B0M BAWUL BBNVY BENPR BHPHI BKEYQ BKKNO BNQBC BPHCQ BVXVI CAG CCPQU CGR COF CS3 CUY CVF DIK DNIVK DPUIP DU5 E3Z EAD EAP EAS EBC EBD EBLON EBS ECM EE. EIF EIOEI EJD EMB EMK EMOBN EPL ESX EX3 F5P FDQFY FEDTE FERAY FIGPU FIZPM FRJ FSGXE FYUFA GX1 HCIFZ HMCUK HVGLF HYE HZ~ IH2 IWAJR J5H JSO JZLTJ KQ8 M1P M41 M7P NAO NAPCQ NPM NQJWS O9- OK1 P2P PQQKQ PROAC PSQYO Q2X RNT RNTTT RPM SNX SNYQT SOHCF SRMVM SV3 SWTZT TAOOD TBHMF TDRGL TR2 TUS UDS UKHRP VH1 W2D WH7 WOW X7M Y6R ZGI ~02 ~8M AAYXX CITATION 7TO 7U9 7XB 8FE 8FH 8FK AZQEC DWQXO GNUQQ H94 K9. LK8 PQEST PQUKI PRINS 7X8 5PM ADTPV AOWAS D8T ZZAVC |
ID | FETCH-LOGICAL-c480t-63a28568312be3faa5c9d2de0e79504754634f18cc56b77e910f5b0d858dd2613 |
IEDL.DBID | RPM |
ISSN | 0007-0920 1532-1827 |
IngestDate | Sun Oct 06 03:38:39 EDT 2024 Tue Sep 17 21:23:35 EDT 2024 Fri Aug 16 23:00:15 EDT 2024 Tue Sep 24 21:21:50 EDT 2024 Thu Sep 26 17:29:26 EDT 2024 Sat Sep 28 08:29:27 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 12 |
Language | English |
License | Note This work is published under the standard license to publish agreement. After 12 months the work will become freely available and the license terms will switch to a Creative Commons Attribution 4.0 International (CC BY 4.0). |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c480t-63a28568312be3faa5c9d2de0e79504754634f18cc56b77e910f5b0d858dd2613 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ORCID | 0000-0002-5184-8971 0000-0001-8588-6506 0000-0002-4718-1414 0000-0003-2498-5206 0000-0002-6865-0224 |
OpenAccessLink | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7722749/ |
PMID | 33020595 |
PQID | 2473301726 |
PQPubID | 41855 |
PageCount | 6 |
ParticipantIDs | swepub_primary_oai_prod_swepub_kib_ki_se_144841364 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7722749 proquest_miscellaneous_2448843643 proquest_journals_2473301726 crossref_primary_10_1038_s41416_020_01111_0 pubmed_primary_33020595 |
PublicationCentury | 2000 |
PublicationDate | 2020-12-08 |
PublicationDateYYYYMMDD | 2020-12-08 |
PublicationDate_xml | – month: 12 year: 2020 text: 2020-12-08 day: 08 |
PublicationDecade | 2020 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: London |
PublicationTitle | British journal of cancer |
PublicationTitleAlternate | Br J Cancer |
PublicationYear | 2020 |
Publisher | Nature Publishing Group Nature Publishing Group UK |
Publisher_xml | – name: Nature Publishing Group – name: Nature Publishing Group UK |
References | AM Bolger (1111_CR16) 2014; 30 C Eklund (1111_CR22) 2018; 101 N Li (1111_CR4) 2011; 128 CE Banister (1111_CR6) 2017; 8 AL Abreu (1111_CR7) 2012; 9 LS Arroyo (1111_CR9) 2013; 58 A Soderlund-Strand (1111_CR19) 2009; 47 K Holl (1111_CR24) 2015; 137 Cancer Genome Atlas Research N, Albert Einstein College of M, Analytical Biological S, Barretos Cancer H, Baylor College of M, Beckman Research Institute of City of H. (1111_CR3) 2017; 543 KU Petry (1111_CR21) 2017; 4 D Bzhalava (1111_CR11) 2013; 8 LS Arroyo Muhr (1111_CR10) 2015; 136 LS Arroyo Muhr (1111_CR8) 2015; 485 C Lagheden (1111_CR13) 2018; 118 M Arbyn (1111_CR27) 2012; 30 J Lei (1111_CR5) 2018; 15 EC Pirog (1111_CR25) 2014; 27 C Lagheden (1111_CR15) 2016; 80 A Molijn (1111_CR23) 2016; 138 M Lindh (1111_CR20) 2007; 40 W Tjalma (1111_CR1) 2018; 10 EC Pirog (1111_CR26) 2017; 141 B Buchfink (1111_CR18) 2015; 12 FJ Sedlazeck (1111_CR17) 2013; 29 JM Walboomers (1111_CR2) 1999; 189 LS Arroyo Muhr (1111_CR12) 2017; 141 LS Arroyo Muhr (1111_CR14) 2020; 101 |
References_xml | – volume: 543 start-page: 378 year: 2017 ident: 1111_CR3 publication-title: Nature doi: 10.1038/nature21386 contributor: fullname: Cancer Genome Atlas Research N, Albert Einstein College of M, Analytical Biological S, Barretos Cancer H, Baylor College of M, Beckman Research Institute of City of H. – volume: 12 start-page: 59 year: 2015 ident: 1111_CR18 publication-title: Nat. Methods doi: 10.1038/nmeth.3176 contributor: fullname: B Buchfink – volume: 189 start-page: 12 year: 1999 ident: 1111_CR2 publication-title: J. Pathol. doi: 10.1002/(SICI)1096-9896(199909)189:1<12::AID-PATH431>3.0.CO;2-F contributor: fullname: JM Walboomers – volume: 118 start-page: 1377 year: 2018 ident: 1111_CR13 publication-title: Br. J. Cancer doi: 10.1038/s41416-018-0053-6 contributor: fullname: C Lagheden – volume: 9 start-page: 262 year: 2012 ident: 1111_CR7 publication-title: Virol. J. doi: 10.1186/1743-422X-9-262 contributor: fullname: AL Abreu – volume: 10 start-page: 107 year: 2018 ident: 1111_CR1 publication-title: Facts Views Vis. Obgyn contributor: fullname: W Tjalma – volume: 101 start-page: 265 year: 2020 ident: 1111_CR14 publication-title: J. Gen. Virol. doi: 10.1099/jgv.0.001374 contributor: fullname: LS Arroyo Muhr – volume: 138 start-page: 409 year: 2016 ident: 1111_CR23 publication-title: Int. J. Cancer doi: 10.1002/ijc.29722 contributor: fullname: A Molijn – volume: 8 start-page: e65953 year: 2013 ident: 1111_CR11 publication-title: PLoS ONE doi: 10.1371/journal.pone.0065953 contributor: fullname: D Bzhalava – volume: 58 start-page: 437 year: 2013 ident: 1111_CR9 publication-title: J. Clin. Virol. doi: 10.1016/j.jcv.2013.07.013 contributor: fullname: LS Arroyo – volume: 4 start-page: 85 year: 2017 ident: 1111_CR21 publication-title: Papillomavirus Res doi: 10.1016/j.pvr.2017.10.003 contributor: fullname: KU Petry – volume: 136 start-page: 2546 year: 2015 ident: 1111_CR10 publication-title: Int J. Cancer doi: 10.1002/ijc.29325 contributor: fullname: LS Arroyo Muhr – volume: 47 start-page: 541 year: 2009 ident: 1111_CR19 publication-title: J. Clin. Microbiol doi: 10.1128/JCM.02007-08 contributor: fullname: A Soderlund-Strand – volume: 101 start-page: 74 year: 2018 ident: 1111_CR22 publication-title: J. Clin. Virol. doi: 10.1016/j.jcv.2018.01.016 contributor: fullname: C Eklund – volume: 27 start-page: 1559 year: 2014 ident: 1111_CR25 publication-title: Mod. Pathol. doi: 10.1038/modpathol.2014.55 contributor: fullname: EC Pirog – volume: 141 start-page: 1653 year: 2017 ident: 1111_CR26 publication-title: Arch. Pathol. Lab Med doi: 10.5858/arpa.2016-0356-RA contributor: fullname: EC Pirog – volume: 80 start-page: 36 year: 2016 ident: 1111_CR15 publication-title: J. Clin. Virol. doi: 10.1016/j.jcv.2016.04.016 contributor: fullname: C Lagheden – volume: 141 start-page: 2498 year: 2017 ident: 1111_CR12 publication-title: Int J. Cancer doi: 10.1002/ijc.31017 contributor: fullname: LS Arroyo Muhr – volume: 137 start-page: 2858 year: 2015 ident: 1111_CR24 publication-title: Int. J. Cancer doi: 10.1002/ijc.29651 contributor: fullname: K Holl – volume: 30 start-page: 2114 year: 2014 ident: 1111_CR16 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btu170 contributor: fullname: AM Bolger – volume: 15 start-page: e1002666 year: 2018 ident: 1111_CR5 publication-title: PLoS Med doi: 10.1371/journal.pmed.1002666 contributor: fullname: J Lei – volume: 128 start-page: 927 year: 2011 ident: 1111_CR4 publication-title: Int J. Cancer doi: 10.1002/ijc.25396 contributor: fullname: N Li – volume: 485 start-page: 283 year: 2015 ident: 1111_CR8 publication-title: Virology doi: 10.1016/j.virol.2015.07.023 contributor: fullname: LS Arroyo Muhr – volume: 30 start-page: F88 year: 2012 ident: 1111_CR27 publication-title: Vaccine doi: 10.1016/j.vaccine.2012.06.095 contributor: fullname: M Arbyn – volume: 29 start-page: 2790 year: 2013 ident: 1111_CR17 publication-title: Bioinformatics doi: 10.1093/bioinformatics/btt468 contributor: fullname: FJ Sedlazeck – volume: 8 start-page: 13375 year: 2017 ident: 1111_CR6 publication-title: Oncotarget doi: 10.18632/oncotarget.14533 contributor: fullname: CE Banister – volume: 40 start-page: 321 year: 2007 ident: 1111_CR20 publication-title: J. Clin. Virol. doi: 10.1016/j.jcv.2007.09.009 contributor: fullname: M Lindh |
SSID | ssj0009087 |
Score | 2.53099 |
Snippet | Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We previously... Abstract Background Human papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR.... BackgroundHuman papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We... BACKGROUNDHuman papillomavirus (HPV) is a necessary cause of cervical cancer, although some invasive cervical cancers may test negative by HPV PCR. We... |
SourceID | swepub pubmedcentral proquest crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
StartPage | 1790 |
SubjectTerms | Adult Aged Aged, 80 and over Alphapapillomavirus - isolation & purification Cervical cancer Cervix Confidence Intervals DNA, Viral - isolation & purification Female High-Throughput Nucleotide Sequencing - methods High-Throughput Nucleotide Sequencing - statistics & numerical data Human papillomavirus Humans Invasiveness Medical screening Medicin och hälsovetenskap Middle Aged Negative Results - statistics & numerical data Nucleotide sequence Paraffin Pilot projects Polymerase chain reaction Quality assurance Reverse Transcriptase Polymerase Chain Reaction Sweden Tumors Uterine Cervical Neoplasms - pathology Uterine Cervical Neoplasms - virology |
SummonAdditionalLinks | – databaseName: ProQuest Central dbid: BENPR link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3di9QwEA_nHYgv4rfVUyL4oEjZNB9t-iR63rEIdyyHJ_dkSNJUi2e3bncF_3tn2uwe5cCHvjRJP2YmM7_JJDOEvLYhqz2zMq05d6m0hUq1rkSaCQVwhDPtSjzgfHqWzy_k50t1uUfm27MwuK1yqxMHRV0tPa6Rz7gswPUGc5vPrMNVAL-eve9-p1g_CuOssZjGLXLAM4kB24OPx2eL8-sEvEyP-TNxaQ4-Ih6gYULPeokjUnSksPI6uNdTI3UDed7cQDlJMzqYppN75G7ElPTDKAT3yV5oH5DbpzFq_pB8-xRCR-OmaTBVtAoYOujpUKCPdrZrrq6Wv-yfZrXp6Zv54utb2rTUD2oEHuxRMlY9bcP3IU04BaBLoRd1f-ni6PwRuTg5_nI0T2NdhdRLzdZpLizXKtci4y6I2lrly4pXgYWiVEwWmCFf1pn2XuWuKAIgilo5Vmmlqwo8LvGY7LfLNjwl1JbOZxXjIQgpHXgbivtC1044bnmR8YS82xLSdGP6DDOEvYU2I9kNkN0MZDcsIYdbWps4lXpzzfiEvNo1wyTAyIZtw3KDfUAPSQHoKiFPRtbsXgeDOWBIlZBiwrRdB0ywPW1pmx9Dom3wPMBpLxPCR_ZOh4CBM_H-zwYv0wfwpKQGXJDLZ___l-fkDkeBw40x-pDsr1eb8ALgzdq9jJL7D0qb-Ic priority: 102 providerName: ProQuest |
Title | Deep sequencing detects human papillomavirus (HPV) in cervical cancers negative for HPV by PCR |
URI | https://www.ncbi.nlm.nih.gov/pubmed/33020595 https://www.proquest.com/docview/2473301726/abstract/ https://search.proquest.com/docview/2448843643 https://pubmed.ncbi.nlm.nih.gov/PMC7722749 http://kipublications.ki.se/Default.aspx?queryparsed=id:144841364 |
Volume | 123 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1ba9swFD60HZS-jN3ntQsa7GFjuLF1seXHLWsJg5RQ1pGnCUmWN7PEMXFS2L_fkS-B0Lc92A-WbBmdY-n7fI4-AbzXLi5spHlYUGpCrlMRSpmzMGYC4QiNpMn8AufZTTK9498WYnEEYlgL0ybtW1NeVsvVZVX-bnMr65UdD3li4_lsgogQyVQ2PobjlLGBog9Ku5HshDL9PzhsrV8pEzE5bniM-CP0jMlvsY48-gxOkdBTxBjicGJ6gDYfJk0eSIu209H1E3jc40jyuXvfp3DkqmdwOusj5c_h51fnatInSuP0RHLnwwUNaTflI7Wuy-VyvdL35WbXkA_T-Y-PpKyIbYcOfLD13rBpSOV-tdLgBMEtwVrE_CXzye0LuLu--j6Zhv1eCqHlMtqGCdNUikSymBrHCq2FzXKau8ilmYh46lXxeRFLa0Vi0tQhiiiEiXIpZJ4jy2Iv4aRaV-41EJ0ZG-cRdY5xbpBhCGpTWRhmqKZpTAP4NHSkqjvJDNWGuplUnQUU9rhqLaCiAC6Gvlb959MoytGunp0mAbzbF6Pj-2iGrtx65-vg2MMZIqoAXnWm2Tc32DSA9MBo-wpeVPuwBH2tFdfufSsA2pn38Bac1FR__U_pD9U4ZE9cIhZI-Jv_bu4czqh3S58nIy_gZLvZubeIdrZmhD6-SPEsJ_EIHn25upnfjlqP_wd9Uv_D |
link.rule.ids | 230,315,733,786,790,891,12083,12250,21416,27957,27958,31754,31755,33301,33302,33779,33780,43345,43614,43840,53827,53829,74102,74371,74659 |
linkProvider | National Library of Medicine |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwED9BJwEviG8CA4zEAwhFc_yRuE8IxqYCa1VNG9oTlp04LNpIQ9Mi8d9zTtxO0SQe8hLbiXLn-H4_3_kO4I1xSZlTI-KSMRsLk8lYqYLHCZcIRxhVduwPOE9n6eRUfD2TZ2HDrQ1hlZs1sVuoi0Xu98j3mMiQeqO5TT80v2NfNcp7V0MJjZuwIzhSlRHsfDqYzY-v0u5S1WfN9Bty-OpwbIZytdeKBMFI7OmTr7eOpHpomq7hzethk4Pkop1BOrwHdwOSJB971d-HG65-ALemwVf-EH58dq4hIVQaDRQpnHcYtKQry0ca01SXl4tf5k-1XLfk7WT-_R2papJ3iwc-OPfzYdmS2v3skoMThLcEexH7l8z3jx_B6eHByf4kDtUU4lwouopTbpiSqeIJs46Xxsh8XLDCUZeNJRWZz4svykTluUxtljnEEaW0tFBSFQXyLP4YRvWidk-BmLHNk4Iy57gQFjmGZHmmSsstMyxLWATvN4LUTZ80Q3fObq50L3aNYted2DWNYHcjax1-oFZfqTuC19tmnPren2Fqt1j7Prj6CI6YKoInvWq2r8PBDJGjjCAbKG3bwafVHrbU1XmXXhv5BlL1cQSsV-9wCJo1He5fVP7SrUP-JBSigVQ8-_-3vILbk5PpkT76Mvv2HO4wP_l8aIzahdFquXYvEOCs7Mswi_8BGkX3ug |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagSBUXxLspBYzEAYSidfyInRNCW1bLo9UKUdQTlu04ENFmw2YXiX_POPFuFVXikEts5zEz9nzjGc8g9NL4rHLE8LSi1KbcSJEqVbI0YwLgCCXKFuGA88lpPj_jH8_FeYx_6mJY5XZN7BfqcunCHvmEcgmmN6jbfFLFsIjF8ext-zsNFaSCpzWW07iJboFWlGGSqulVuEdB1JA_M2zNwUfEAzSEqUnHM4AlaTCkQuV1MK_HSuoa8rweQDlKM9qrptlddCdiSvxuEIJ76IZv7qP9k-g1f4C-H3vf4hg0DaoKlz64DjrcF-jDrWnri4vlpflTrzYdfjVffHuN6wa7fhmBB7sgGasON_5HnyYcA9DF0Avbv3gx_fIQnc3ef53O01hXIXVckXWaM0OVyBXLqPWsMka4oqSlJ14WgnAZMuTzKlPOidxK6QFRVMKSUglVlmBxsUdor1k2_gBhU1iXlYR6zzi3YG0I6qSqLLPUUJnRBL3ZElK3Q_oM3bu9mdID2TWQXfdk1yRBR1ta6ziVOn3F-AS92DXDJAieDdP45Sb0gXWIM0BXCXo8sGb3OhhMAUOKBMkR03YdQoLtcUtT_-wTbYPlAUZ7kSA6sHc8BBScjvd_1eHSnQdLiivABTk__P-_PEf7IL7684fTT0_QbRpkL8TIqCO0t15t_FNAOmv7rBfhf0Sa-lQ |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Deep+sequencing+detects+human+papillomavirus+%28HPV%29+in+cervical+cancers+negative+for+HPV+by+PCR&rft.jtitle=British+journal+of+cancer&rft.au=Arroyo+M%C3%BChr%2C+Laila+Sara&rft.au=Lagheden%2C+Camilla&rft.au=Lei%2C+Jiayao&rft.au=Eklund%2C+Carina&rft.date=2020-12-08&rft.eissn=1532-1827&rft.volume=123&rft.issue=12&rft.spage=1790&rft.epage=1795&rft_id=info:doi/10.1038%2Fs41416-020-01111-0&rft.externalDBID=NO_FULL_TEXT |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0007-0920&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0007-0920&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0007-0920&client=summon |