Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis

• Published in: Developmental and comparative immunology Vol. 68; pp. 98 - 107
• Main Authors: Talactac, Melbourne Rio, Yada, Yurika, Yoshii, Kentaro, Hernandez, Emmanuel Pacia, Kusakisako, Kodai, Maeda, Hiroki, Galay, Remil Linggatong, Fujisaki, Kozo, Mochizuki, Masami, Tanaka, Tetsuya
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.03.2017; Elsevier Science Ltd
• Subjects: adults; Animals; Antiinfectives and antibacterials; Antimicrobial peptide; Antimicrobial peptides; Antiviral activity; antiviral properties; Arachnids; bacteria; Biological activity; Cells, Cultured; Cloning, Molecular; Containment of Biohazards; Defensins; Defensins - genetics; Defensins - metabolism; Disease Models, Animal; Ectoparasites; Encephalitis Viruses, Tick-Borne - immunology; Encephalitis, Tick-Borne - immunology; Expressed sequence tags; Fungi; Gene expression; Gene silencing; Genes; Haemaphysalis longicornis; HEdefensin; hematophagy; Hemolymph; Hemolymph - metabolism; Humans; Immunity; Immunity, Innate - genetics; Immunology; Innate immunity; Insect Proteins - genetics; Insect Proteins - metabolism; Ixodidae - immunology; Langat virus; Organs; Parasites; Peptides; RNA-mediated interference; Sequence Alignment; Ticks; viral load; Viruses; PBS; dsRNA; HEdefensin; Langat virus; RNAi; Haemaphysalis longicornis; Luc; Antimicrobial peptide

Tick defensins are antimicrobial peptides that play a major role in the innate immunity of ticks by providing a direct antimicrobial defense. In this study, we identified and characterized a defensin-like encoding gene, HEdefensin, from the expressed sequence tags (EST) database of hemolymph from th...