Proteomic analysis of influenza haemagglutinin-specific antibodies following vaccination reveals convergent immunoglobulin variable region signatures

• Published in: Vaccine Vol. 35; no. 42; pp. 5576 - 5580
• Main Authors: Adamson, Penelope J., Al Kindi, Mahmood A., Wang, Jing J., Colella, Alex D., Chataway, Timothy K., Petrovsky, Nikolai, Gordon, Tom P., Gordon, David L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 09.10.2017; Elsevier Limited
• Subjects: Adult; Aged; Amino Acid Sequence; Antibodies; Antibodies, Viral - immunology; Antigens; blood serum; Chains; Feasibility studies; Female; Gene families; genes; germ cells; Hemagglutinins; Hemagglutinins - immunology; Humans; Immunoglobulin G; Immunoglobulin Variable Region - immunology; Immunoglobulins; Infections; Influenza; Influenza A; Influenza, Human - immunology; Influenza, Human - prevention & control; Lupus; Male; Mass spectrometric sequencing; Middle Aged; Mutation; Peptides; Proteins; proteome; Proteome - immunology; Proteomics; Proteomics - methods; Public health; Serum antibody repertoire; Signatures; Vaccination; Vaccination - methods; Vaccines; Variable region; D; Vaccination; H; rHA; J; K; L; PBMC; CDR; BCR; IMGT; Proteomics; IgV; Influenza A; Serum antibody repertoire; Mass spectrometric sequencing

•Anti-H1 Igs were purified from sera following H1N1pdm09 influenza A vaccination.•Proteome of secreted anti-H1 Igs was analysed by mass spectrometric sequencing.•Anti-H1 Igs express convergent antibody response in unrelated individuals. Analysis of the anti-haemagglutinin serum antibody proteome fro...