Subretinal Pigment Epithelial Deposition of Drusen Components Including Hydroxyapatite in a Primary Cell Culture Model

• Published in: Investigative ophthalmology & visual science Vol. 58; no. 2; pp. 708 - 719
• Main Authors: Pilgrim, Matthew G., Lengyel, Imre, Lanzirotti, Antonio, Newville, Matt, Fearn, Sarah, Emri, Eszter, Knowles, Jonathan C., Messinger, Jeffrey D., Read, Russell W., Guidry, Clyde, Curcio, Christine A.
• Format: Journal Article
• Language: English
• Published: United States Association for Research in Vision and Ophthalmology 01.02.2017; The Association for Research in Vision and Ophthalmology
• Subjects: Animals; Disease Models, Animal; Durapatite - metabolism; Epithelial Cells - metabolism; Fluorescence; Immunohistochemistry; Macular Degeneration - metabolism; Microscopy, Electron; Pigment Epithelium of Eye - cytology; Pigment Epithelium of Eye - metabolism; Primary Cell Culture; Retinal Cell Biology; Retinal Drusen - metabolism; Spectrometry, Mass, Secondary Ion; Swine; X-Ray Diffraction

Extracellular deposits containing hydroxyapatite, lipids, proteins, and trace metals that form between the basal lamina of the RPE and the inner collagenous layer of Bruch's membrane are hallmarks of early AMD. We examined whether cultured RPE cells could produce extracellular deposits containi...