Comparative effect of genistein and daidzein on the expression of MCP-1, eNOS, and cell adhesion molecules in TNF-α-stimulated HUVECs

• Published in: Nutrition research and practice Vol. 5; no. 5; pp. 381 - 388
• Main Authors: Cho, H.Y., Paik Institute for Clinical Research, Inje University, Busan, Republic of Korea, Park, C.M., Inje University, Gimhae, Republic of Korea, Kim, M.J., Silla University, Busan, Republic of Korea, Chinzorig, Radnaabazar, Inje University, Gimhae, Republic of Korea, Cho, C.W., Inje University, Gimhae, Republic of Korea, Song, Y.S., Inje University, Gimhae, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국영양학회 01.10.2011; The Korean Nutrition Society and the Korean Society of Community Nutrition
• Subjects: cell adhesion molecules; eNOS; GENISTEIN; GENISTEINA; GENISTEINE; MCP-1; NF kappa B; Original Research; 생활과학; MCP-1; cell adhesion molecules; eNOS; NFκB; Genistein
• ISSN: 1976-1457; 2005-6168; 2005-6168
• DOI: 10.4162/nrp.2011.5.5.381

We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-α-stimulated human umbilical vascular endothelial cells (HUVECs). TNF-α exposure significantly increased expression of monocyte chemoattractant protein (MCP)-1, vascular adhesion molecule (VCAM)-1, and intercellular adhesion molecule-1. Genistein significantly decreased MCP-1 and VCAM-1 production in a dose-dependent manner, whereas CAM expression was not significantly lowered by genistein treatment. However, daidzein slightly decreased MCP-1 production. The effects of genistein and daidzein on MCP-1 secretion coincided with mRNA expression. Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-α exposure. A low concentration of isoflavones significantly inhibited nuclear factor (NF)κB activation, whereas a high dose slightly ameliorated these inhibitive effects. These results suggest that genistein had a stronger effect on MCP-1 and eNOS expression than that of daidzein. Additionally, NFκB transactivation might be partially related to the down-regulation of these mRNAs in TNF-α-stimulated HUVECs.