Low-density lipoproteins interact with liposome-binding sites on the cell surface

Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that...

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Bibliographic Details
Published inFEBS letters Vol. 287; no. 1; pp. 19 - 22
Main Authors Galkina, S.I., Ivanov, V.V., Preobrazhensky, S.N., Margolis, L.B., Bergelson, L.D.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 05.08.1991
Elsevier
Subjects
1,2-Dipalmitoylphosphatidylcholine - metabolism
Animals
Binding Sites
Binding, Competitive
Biological and medical sciences
Cell Membrane - metabolism
Cell receptors
Cell structures and functions
dipalmitoyl PC
DPPC
Embryo, Mammalian
fibroblasts
Fibroblasts - metabolism
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Humans
Immunohistochemistry
LDL
Lipoproteins, LDL - metabolism
liposomes
Liposomes - metabolism
Low-density lipoprotein: Liposome
low-density lipoproteins
Mice
Microscopy, Electron
Microscopy, Electron, Scanning
Miscellaneous
Molecular and cellular biology
phosphatidylcholine
PC
Low-density lipoprotein: Liposome
LDL
DPPC
Lipoprotein LDL
Scanning electron microscopy
Vertebrata
Membrane receptor
Mammalia
Mouse
Rodentia
Liposome
Molecular interaction
Binding site
Fibroblast
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Summary:Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding sites could contribute to LDL interaction with the cell surface via phospholipid molecules of LDL particles. To check this hypothesis we studied the competitive interaction of human LDL and DPPC liposomes with mouse embryo fibroblasts depleted of apo-B receptors by preliminary incubation with LDL. We have found that after removal of the liposome-binding sites from cell lamellae these areas of the cell surface lose their ability to bind LDL.
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ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)80006-O