SP22: a novel fertility protein from a highly conserved gene family

Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA library. While two of these cDNA sequences differed only in their 5′ untranslated regions, a third cDNA was predicted to contain an addition...

Full description

Saved in:
Bibliographic Details
Published inJournal of andrology Vol. 19; no. 4; pp. 385 - 393
Main Authors Welch, J. E, Barbee, R. R, Roberts, N. L, Suarez, J. D, Klinefelter, G. R
Format Journal Article
LanguageEnglish
Published Oxford, UK Am Soc Andrology 01.07.1998
Blackwell Publishing Ltd
American Society of Andrology
Subjects
Online AccessGet full text

Cover

Loading…
Abstract Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA library. While two of these cDNA sequences differed only in their 5′ untranslated regions, a third cDNA was predicted to contain an additional 13 amino acids of coding sequence. Amino acid sequences obtained following Edman degradation of purified SP22 protein and cDNA sequence data both indicated that SP22 was a member of a highly conserved and widely expressed gene family found in organisms as diverse as human and Escherichia coli. Interestingly, while a 1‐kb mRNA transcript was widely expressed in somatic tissues, a unique pattern of testicular expression was observed, including the appearance of a novel 1.5‐kb transcript and an increase in the abundance of the 1‐kb transcript during spermatogenic cell development. Anti‐SP22 peptide antiserum was shown to recognize a family of 22‐kDa proteins on western blots of detergent‐extracted cauda epididymal sperm protein, suggesting that multiple charge variants of SP22 coexist. Moreover, affinity‐purified anti‐SP22 peptide immunoglobulin localized in a highly specific manner to the anterior‐ventral surface of the equatorial segment of the sperm head. This is an extremely intriguing finding as SP22 was originally shown to be highly correlated with, and predictive of, the fertilizing ability of cauda epididymal sperm. Although no conclusive function has been attributed to any members of the SP22 gene family, the localization of SP22 over a discrete region of the sperm head suggests a pivotal role in sperm—egg interactions.
AbstractList Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA library. While two of these cDNA sequences differed only in their 5' untranslated regions, a third cDNA was predicted to contain an additional 13 amino acids of coding sequence. Amino acid sequences obtained following Edman degradation of purified SP22 protein and cDNA sequence data both indicated that SP22 was a member of a highly conserved and widely expressed gene family found in organisms as diverse as human and Escherichia coli. Interestingly, while a 1-kb mRNA transcript was widely expressed in somatic tissues, a unique pattern of testicular expression was observed, including the appearance of a novel 1.5-kb transcript and an increase in the abundance of the 1-kb transcript during spermatogenic cell development. Anti-SP22 peptide antiserum was shown to recognize a family of 22-kDa proteins on western blots of detergent-extracted cauda epididymal sperm protein, suggesting that multiple charge variants of SP22 coexist. Moreover, affinity-purified anti-SP22 peptide immunoglobulin localized in a highly specific manner to the anterior-ventral surface of the equatorial segment of the sperm head. This is an extremely intriguing finding as SP22 was originally shown to be highly correlated with, and predictive of, the fertilizing ability of cauda epididymal sperm. Although no conclusive function has been attributed to any members of the SP22 gene family, the localization of SP22 over a discrete region of the sperm head suggests a pivotal role in sperm-egg interactions.
Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA library. While two of these cDNA sequences differed only in their 5′ untranslated regions, a third cDNA was predicted to contain an additional 13 amino acids of coding sequence. Amino acid sequences obtained following Edman degradation of purified SP22 protein and cDNA sequence data both indicated that SP22 was a member of a highly conserved and widely expressed gene family found in organisms as diverse as human and Escherichia coli . Interestingly, while a 1‐kb mRNA transcript was widely expressed in somatic tissues, a unique pattern of testicular expression was observed, including the appearance of a novel 1.5‐kb transcript and an increase in the abundance of the 1‐kb transcript during spermatogenic cell development. Anti‐SP22 peptide antiserum was shown to recognize a family of 22‐kDa proteins on western blots of detergent‐extracted cauda epididymal sperm protein, suggesting that multiple charge variants of SP22 coexist. Moreover, affinity‐purified anti‐SP22 peptide immunoglobulin localized in a highly specific manner to the anterior‐ventral surface of the equatorial segment of the sperm head. This is an extremely intriguing finding as SP22 was originally shown to be highly correlated with, and predictive of, the fertilizing ability of cauda epididymal sperm. Although no conclusive function has been attributed to any members of the SP22 gene family, the localization of SP22 over a discrete region of the sperm head suggests a pivotal role in sperm—egg interactions.
Author Welch, J. E
Suarez, J. D
Klinefelter, G. R
Barbee, R. R
Roberts, N. L
Author_xml – sequence: 1
  fullname: Welch, J. E
– sequence: 2
  fullname: Barbee, R. R
– sequence: 3
  fullname: Roberts, N. L
– sequence: 4
  fullname: Suarez, J. D
– sequence: 5
  fullname: Klinefelter, G. R
BackLink http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2397760$$DView record in Pascal Francis
https://www.ncbi.nlm.nih.gov/pubmed/9733139$$D View this record in MEDLINE/PubMed
BookMark eNqVkMtO3DAYhS1ERQfKIyBFVdtdpr7ETswKNC29CLWVWtaW4_wGj5wE7Awhb4-jiWbflS2fi4--U3Tc9R0g9J7gNcGYft6uiWQyL0SRHqSs1kONKaZy_XKEVgfpGK0wkSJngvG36DTGbcpiUrITdCJLxgiTK7T5-4fSy0xnXf8MPrMQBufdMGWPoR_AdZkNfZvkB3f_4KfM9F2E8AxNdg8dZFa3zk_v0BurfYTz5TxDdzdf_22-57e_v_3YXN_mpqiwzGshmOZQMVYSY0TdaNpwbSpelAKkhUJqiuuiqSvKK4G1rjnlRIDVdSOZsewMfdr3pm1PO4iDal004L3uoN9FVTKJK8LLZLzcG03oYwxg1WNwrQ6TIljNBNVWzZjUjEnNBNVCUL2k8MXyy65uoTlEF2RJ_7DoOhrtbdCdcfFgo0yWpcDJdrW3jc7D9B8D1M_rX1_ma6r4uK-Y4Y8ugIqt9j7tImocR5LiilWcvQJBZZw_
CODEN JOAND3
CitedBy_id crossref_primary_10_1016_j_anireprosci_2010_02_007
crossref_primary_10_1002_j_1939_4640_2000_tb03427_x
crossref_primary_10_1038_sj_cdd_4401704
crossref_primary_10_1002_jemt_10319
crossref_primary_10_1111_rda_12485
crossref_primary_10_1155_2018_6136075
crossref_primary_10_1016_j_neulet_2007_11_027
crossref_primary_10_1002_j_1939_4640_2001_tb02593_x
crossref_primary_10_1002_jand_2002_23_1_48
crossref_primary_10_1016_j_bbrep_2016_12_010
crossref_primary_10_1016_j_molbiopara_2018_05_003
crossref_primary_10_1074_jbc_M311017200
crossref_primary_10_1177_153537020523001005
crossref_primary_10_1016_j_febslet_2008_06_043
crossref_primary_10_1111_andr_13464
crossref_primary_10_1016_j_anireprosci_2010_11_015
crossref_primary_10_1007_s10815_017_1033_0
crossref_primary_10_1002_mrd_20085
crossref_primary_10_1016_j_anireprosci_2007_11_021
crossref_primary_10_1016_S0014_5793_03_00764_6
crossref_primary_10_1016_j_fertnstert_2011_04_048
crossref_primary_10_1016_S1096_4959_02_00098_2
crossref_primary_10_1074_jbc_M804243200
crossref_primary_10_1002__SICI_1098_2795_200002_55_2_189__AID_MRD8_3_0_CO_2_O
crossref_primary_10_1186_1477_5956_1_4
crossref_primary_10_1093_toxsci_kfi278
crossref_primary_10_1016_j_cbd_2006_10_004
crossref_primary_10_1093_molbev_msab056
crossref_primary_10_1016_j_reprotox_2023_108343
crossref_primary_10_1111_j_1439_0531_2008_01313_x
crossref_primary_10_1002_jcb_20159
crossref_primary_10_1074_jbc_M305878200
crossref_primary_10_1530_REP_14_0222
crossref_primary_10_1016_S0378_1119_00_00590_4
crossref_primary_10_1586_1744666X_4_1_113
crossref_primary_10_1007_s00335_022_09958_9
crossref_primary_10_1073_pnas_0911175107
crossref_primary_10_1074_jbc_M101730200
crossref_primary_10_2164_jandrol_107_002550
crossref_primary_10_1021_acs_jproteome_0c01044
crossref_primary_10_1002_mrd_10360
crossref_primary_10_1073_pnas_1133288100
crossref_primary_10_1016_j_neulet_2005_07_053
crossref_primary_10_1002_mrd_20065
crossref_primary_10_1016_j_bbrc_2004_05_187
crossref_primary_10_1155_2013_683920
crossref_primary_10_1248_bpb_25_853
crossref_primary_10_1002_j_1939_4640_2001_tb02192_x
crossref_primary_10_1042_BJ20140051
crossref_primary_10_1038_cdd_2008_26
crossref_primary_10_1038_sj_embor_7400074
crossref_primary_10_1080_10495398_2018_1432488
crossref_primary_10_1074_jbc_M304221200
crossref_primary_10_1080_10715760500260348
Cites_doi 10.1016/S0022-2836(05)80360-2
10.1002/(SICI)1098-2795(199804)49:4<374::AID-MRD4>3.0.CO;2-K
10.1002/j.1939-4640.1997.tb01894.x
10.1074/jbc.270.6.2411
10.1002/(SICI)1098-2795(199702)46:2<168::AID-MRD7>3.0.CO;2-O
10.1006/geno.1996.0177
10.1126/science.322279
10.1095/biolreprod30.3.721
10.1095/biolreprod49.6.1202
10.1128/MCB.13.10.6547
10.1095/biolreprod46.5.869
10.1126/science.7541556
10.1073/pnas.74.12.5463
10.1016/0167-4781(96)00077-2
10.1002/mrd.1080340209
10.1006/bbrc.1997.6132
10.1095/biolreprod55.1.200
10.1095/biolreprod56.5.1142
ContentType Journal Article
Copyright 1998 American Society of Andrology
1998 INIST-CNRS
Copyright_xml – notice: 1998 American Society of Andrology
– notice: 1998 INIST-CNRS
DBID IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
DOI 10.1002/j.1939-4640.1998.tb02029.x
DatabaseName Pascal-Francis
Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
MEDLINE - Academic
DatabaseTitleList MEDLINE

MEDLINE - Academic
CrossRef
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Anatomy & Physiology
EISSN 1939-4640
EndPage 393
ExternalDocumentID 10_1002_j_1939_4640_1998_tb02029_x
9733139
2397760
JAND2029
www19_4_385
Genre miscellaneous
Research Support, U.S. Gov't, Non-P.H.S
Journal Article
GroupedDBID -
1OB
1OC
24P
2WC
31
53G
55
5GY
5RE
8-1
AAKDD
AAZKR
ABCUV
ABFLS
ABHUG
ACGFS
ACMXC
ACPOU
ACXQS
ADACO
ADAWD
ADBBV
ADDAD
ADEOM
ADKZR
AEUQT
AFBPY
AFPWT
AGCAB
AGJLS
AJYGW
ALMA_UNASSIGNED_HOLDINGS
ALUQN
AZFZN
BAWUL
BRXPI
CS3
DCZOG
DIK
DRFUL
DRMAN
DRSTM
DU5
E3Z
EBS
EJD
F5P
GJ
H13
LATKE
LITHE
LOXES
LUTES
LYRES
MEWTI
MRFUL
MRSTM
MSFUL
MSMAN
MSSTM
MXFUL
MXMAN
MXSTM
OK1
P2P
SJN
TND
VH1
WIH
WIJ
WIK
WIN
WOQ
WRC
X7M
ZXP
---
.55
.GJ
31~
AANLZ
AAXRX
ABJNI
ABQWH
ABXGK
ACAHQ
ACCZN
ACXBN
ADBTR
ADMGS
ADOZA
AEIGN
AEUYR
AFFPM
AFGKR
AHBTC
AI.
AIACR
AITYG
AIURR
AMYDB
AYCSE
GODZA
HGLYW
LEEKS
MRMAN
P6G
TR2
W8F
WXSBR
ZGI
AAUGY
AFVGU
IQODW
CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7X8
ID FETCH-LOGICAL-c4809-b663a5e83371cc6bda2d5ac85476e9fe49a20b4db825860aab52516efabd93cf3
ISSN 0196-3635
IngestDate Sat Oct 26 00:24:54 EDT 2024
Fri Aug 23 00:35:12 EDT 2024
Sat Sep 28 07:35:29 EDT 2024
Sun Oct 29 17:09:46 EDT 2023
Sat Aug 24 00:56:02 EDT 2024
Tue Nov 10 19:48:23 EST 2020
IsDoiOpenAccess false
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 4
Keywords Reproduction
Spermatozoa
Fertilizing ability
Nucleotide sequence
Semen
Germinal cell
Localization
Fecundity
Protein
Language English
License CC BY 4.0
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c4809-b663a5e83371cc6bda2d5ac85476e9fe49a20b4db825860aab52516efabd93cf3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
OpenAccessLink https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/j.1939-4640.1998.tb02029.x
PMID 9733139
PQID 73908157
PQPubID 23479
PageCount 9
ParticipantIDs proquest_miscellaneous_73908157
crossref_primary_10_1002_j_1939_4640_1998_tb02029_x
pubmed_primary_9733139
pascalfrancis_primary_2397760
wiley_primary_10_1002_j_1939_4640_1998_tb02029_x_JAND2029
highwire_smallpub1_www19_4_385
PublicationCentury 1900
PublicationDate July‐August 1998
PublicationDateYYYYMMDD 1998-07-01
PublicationDate_xml – month: 07
  year: 1998
  text: July‐August 1998
PublicationDecade 1990
PublicationPlace Oxford, UK
PublicationPlace_xml – name: Oxford, UK
– name: San Fransisco, CA
– name: United States
PublicationTitle Journal of andrology
PublicationTitleAlternate J Androl
PublicationYear 1998
Publisher Am Soc Andrology
Blackwell Publishing Ltd
American Society of Andrology
Publisher_xml – name: Am Soc Andrology
– name: Blackwell Publishing Ltd
– name: American Society of Andrology
References 1998; 49
1993; 13
1984; 30
1993; 34
1990; 215
1998; 19
1993; 49
1997; 74
1997; 231
1997; 46
1997; 56
1997; 18
1996
1995; 269
1992; 46
1996; 1307
1977; 196
1995; 270
1989
1996; 55
1996; 33
Sambrook J. (e_1_2_1_19_1) 1989
Amann RP (e_1_2_1_3_1) 1998; 19
Sanger F. (e_1_2_1_20_1) 1997; 74
e_1_2_1_7_1
e_1_2_1_8_1
e_1_2_1_5_1
e_1_2_1_6_1
e_1_2_1_12_1
e_1_2_1_13_1
e_1_2_1_10_1
e_1_2_1_21_1
e_1_2_1_2_1
e_1_2_1_11_1
e_1_2_1_22_1
e_1_2_1_16_1
e_1_2_1_17_1
Backstrom AD. (e_1_2_1_4_1) 1996
e_1_2_1_14_1
e_1_2_1_15_1
e_1_2_1_9_1
e_1_2_1_18_1
References_xml – volume: 33
  start-page: 151
  year: 1996
  end-page: 152
  article-title: The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression
  publication-title: Genomics
– volume: 19
  start-page: 102A
  year: 1998
  article-title: Responses of mammalian sperm exposed to synthetic FertPlus™ peptide
  publication-title: J Androl (Suppl)
– volume: 270
  start-page: 2411
  year: 1995
  end-page: 2414
  article-title: Exon recognition in vertebrate splicing
  publication-title: J Biol Chem
– volume: 13
  start-page: 6547
  year: 1993
  end-page: 6557
  article-title: Binding of a phosphoprotein to the 3′ untranslated region of the mouse protamine 2 mRNA temporally represses its translation
  publication-title: Mol Cell Biol
– volume: 49
  start-page: 374
  year: 1998
  end-page: 385
  article-title: Mouse spermatogenic cell‐specific type 1 hexokinase (mHk1‐s) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1‐S protein is localized mainly in the sperm tail
  publication-title: Mol Reprod Dev
– volume: 46
  start-page: 869
  year: 1992
  end-page: 878
  article-title: Expression of a glyceraldehyde 3‐phosphate dehydrogenase gene specific to mouse spermatogenic cells
  publication-title: Biol Reprod
– year: 1989
– volume: 34
  start-page: 175
  year: 1993
  end-page: 182
  article-title: Improved preservation of rat epididymal sperm for high‐resolution low‐voltage scanning electron microscopy (HR‐LVSEM)
  publication-title: Mol Reprod Dev
– volume: 269
  start-page: 83
  year: 1995
  end-page: 89
  article-title: Interaction of a tyrosine kinase from human sperm with the zona pellucida at fertilization
  publication-title: Science
– volume: 1307
  start-page: 263
  year: 1996
  end-page: 266
  article-title: Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17
  publication-title: Biochim Biophys Acta
– year: 1996
– volume: 49
  start-page: 1202
  year: 1993
  end-page: 1207
  article-title: Fertility‐associated proteins in Holstein bull seminal plasma
  publication-title: Biol Reprod
– volume: 231
  start-page: 509
  year: 1997
  end-page: 513
  article-title: DJ‐1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras
  publication-title: Biochem Biophys Res Commun
– volume: 215
  start-page: 403
  year: 1990
  end-page: 410
  article-title: Basic local alignment search tool
  publication-title: J Mol Biol
– volume: 46
  start-page: 168
  year: 1997
  end-page: 175
  article-title: Binding of boar spermatozoa to porcine oocytes: effect of low molecular weight 17‐kda protein
  publication-title: Mol Reprod Dev
– volume: 30
  start-page: 721
  year: 1984
  end-page: 730
  article-title: Monoclonal antibodies to rabbit sperm autoantigens. I. inhibition of in vitro fertilization and localization on the egg
  publication-title: Biol Reprod
– volume: 74
  start-page: 5463
  year: 1997
  end-page: 5467
  article-title: DNA sequencing with chain terminating inhibitors
  publication-title: Proc Natl Acad Sci USA
– volume: 56
  start-page: 1142
  year: 1997
  end-page: 1146
  article-title: Reversible contraceptive effect of PH‐20 immunization in male guinea pigs
  publication-title: Biol Reprod
– volume: 196
  start-page: 180
  year: 1977
  article-title: Screening λ recombinant clones by hybridization to single plaques in situ
  publication-title: Science
– volume: 18
  start-page: 139
  year: 1997
  end-page: 150
  article-title: Discriminant analysis indicates a single sperm protein (SP22) is predictive of fertility following exposure to epididymal toxicants
  publication-title: J Androl
– volume: 55
  start-page: 200
  year: 1996
  end-page: 206
  article-title: Mammalian sperm—egg fusion: the development of rat oolemma fusibility during oogenesis involves the appearance of binding sites for sperm protein “DE.”
  publication-title: Biol Reprod
– volume-title: Molecular Cloning: A Laboratory Manual
  year: 1989
  ident: e_1_2_1_19_1
  contributor:
    fullname: Sambrook J.
– ident: e_1_2_1_2_1
  doi: 10.1016/S0022-2836(05)80360-2
– ident: e_1_2_1_15_1
  doi: 10.1002/(SICI)1098-2795(199804)49:4<374::AID-MRD4>3.0.CO;2-K
– ident: e_1_2_1_11_1
  doi: 10.1002/j.1939-4640.1997.tb01894.x
– ident: e_1_2_1_6_1
  doi: 10.1074/jbc.270.6.2411
– ident: e_1_2_1_8_1
  doi: 10.1002/(SICI)1098-2795(199702)46:2<168::AID-MRD7>3.0.CO;2-O
– ident: e_1_2_1_14_1
  doi: 10.1006/geno.1996.0177
– ident: e_1_2_1_5_1
  doi: 10.1126/science.322279
– ident: e_1_2_1_17_1
  doi: 10.1095/biolreprod30.3.721
– ident: e_1_2_1_10_1
  doi: 10.1095/biolreprod49.6.1202
– ident: e_1_2_1_12_1
  doi: 10.1128/MCB.13.10.6547
– ident: e_1_2_1_22_1
  doi: 10.1095/biolreprod46.5.869
– ident: e_1_2_1_7_1
  doi: 10.1126/science.7541556
– volume: 74
  start-page: 5463
  year: 1997
  ident: e_1_2_1_20_1
  article-title: DNA sequencing with chain terminating inhibitors
  publication-title: Proc Natl Acad Sci USA
  doi: 10.1073/pnas.74.12.5463
  contributor:
    fullname: Sanger F.
– ident: e_1_2_1_13_1
  doi: 10.1016/0167-4781(96)00077-2
– ident: e_1_2_1_21_1
  doi: 10.1002/mrd.1080340209
– volume: 19
  start-page: 102A
  year: 1998
  ident: e_1_2_1_3_1
  article-title: Responses of mammalian sperm exposed to synthetic FertPlus™ peptide
  publication-title: J Androl (Suppl)
  contributor:
    fullname: Amann RP
– ident: e_1_2_1_16_1
  doi: 10.1006/bbrc.1997.6132
– ident: e_1_2_1_9_1
  doi: 10.1095/biolreprod55.1.200
– ident: e_1_2_1_18_1
  doi: 10.1095/biolreprod56.5.1142
– volume-title: TYE Gene Product (Thiamin Phosphate Synthase [E.C. 2.5.1.3])
  year: 1996
  ident: e_1_2_1_4_1
  contributor:
    fullname: Backstrom AD.
SSID ssj0020173
Score 1.6839037
Snippet Three nucleotide sequences encoding SP22, a protein originally identified in detergent extracts of cauda epididymal sperm, were isolated from a rat testis cDNA...
SourceID proquest
crossref
pubmed
pascalfrancis
wiley
highwire
SourceType Aggregation Database
Index Database
Publisher
StartPage 385
SubjectTerms Amino Acid Sequence
Animals
Antibodies, Monoclonal
Base Sequence
Biological and medical sciences
Blotting, Northern
Blotting, Western
DNA, Complementary - genetics
Electrophoresis, Polyacrylamide Gel
Fertility
Fundamental and applied biological sciences. Psychology
Immunohistochemistry
Male
Mammalian male genital system
Molecular Sequence Data
Morphology. Physiology
Proteins - genetics
Proteins - metabolism
Rats
Rats, Sprague-Dawley
Spermatozoa - metabolism
Vertebrates: reproduction
Title SP22: a novel fertility protein from a highly conserved gene family
URI http://www.andrologyjournal.org/cgi/content/abstract/19/4/385
https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fj.1939-4640.1998.tb02029.x
https://www.ncbi.nlm.nih.gov/pubmed/9733139
https://search.proquest.com/docview/73908157
Volume 19
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3fb9MwELZgvPCCgDERYMMPiJcq4NpxEvNW7YdGGQWxVtqbZSeONKlL0dpRxl_PnZ2krdgE4yWK0taJ7vvsni933xHyhknucqNc7Jgs4gQ2FbGyzMB0l_3CS-NWGBr4PEqPJ8nwTJ6tuvT56pKFfVf8urGu5H9QhWuAK1bJ3gHZblC4AOeALxwBYTj-E8anXzkP1cr17Ieb9ipMkvZ-tZdfOK9D9YjpoSjx9BpTzDEICz4mDOma4MYt7qlBJYONkLubhrZRw96aOuelDZk831aZhyFZ2_Nj1Otiy6fAxRCtHjZZxuWq-C5rYw1N-FGlsUiDwEi3fqo1niRri6EIzXia_1UROiH-sWQHCVhYshW-k04TX0CZY8SaM67aZM51nezRF300OTnR48Oz8X3ygGdKYjLnwcdP3VabNbkF7fOu5Gbf336nTdeklYvGbFkzhwlThU4nN21FNnc23jUZPyaPGtDoIBDkCbnn6qdke1Cbxezimr6lPsvXY7lN9pEzH6ihnjG0YwxtGEORMfBxYAztGEORMTQw5hmZHB2O94_jppFGXCQ5U7EFt9JIlwuR9YsitaXhpTRFLpMsdapyiTKc2aS0OZd5yoyxEtze1FXGlkoUldghW_Wsds8JZYljlhd9h7qCqSlgXltWSmVEwplIyoiI1ob6e9BL0UEZm-M-Eyyv0fIaLa8by-ufEdlrza3nF2Y6BbP29XK57MPXNfAoIrsbIHRjc9zApCwir1tQNKyP-NLL1G52NdeZUOD1yiwiOwGr7qcK25UKFRHlsbvD4-rhYHSApy_-etuX5OFqIr0iW4vLK7cLbuzC7nnG_gZpQZTb
link.rule.ids 315,786,790,27955,27956
linkProvider Flying Publisher
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=SP22%3A+a+novel+fertility+protein+from+a+highly+conserved+gene+family&rft.jtitle=Journal+of+andrology&rft.au=Welch%2C+J+E&rft.au=Barbee%2C+R+R&rft.au=Roberts%2C+N+L&rft.au=Suarez%2C+J+D&rft.date=1998-07-01&rft.issn=0196-3635&rft.volume=19&rft.issue=4&rft.spage=385&rft.epage=393&rft_id=info:doi/10.1002%2Fj.1939-4640.1998.tb02029.x&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0196-3635&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0196-3635&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0196-3635&client=summon