An E3 ubiquitin ligase from Nicotiana benthamiana targets the replicase of Bamboo mosaic virus and restricts its replication

• Published in: Molecular plant pathology Vol. 20; no. 5; pp. 673 - 684
• Main Authors: Chen, I‐Hsuan, Chang, Jui‐En, Wu, Chen‐Yu, Huang, Ying‐Ping, Hsu, Yau‐Huei, Tsai, Ching‐Hsiu
• Format: Journal Article
• Language: English
• Published: England John Wiley & Sons, Inc 01.05.2019; John Wiley and Sons Inc
• Subjects: Bamboo mosaic virus; Capsid Proteins - metabolism; complementary DNA; DNA, Complementary - genetics; E3 ubiquitin ligase; Fluorescence; fluorescent proteins; Gene Expression Regulation, Plant - drug effects; gene silencing; Genes; Genes, Plant; Genetic engineering; Health aspects; Infection; Leupeptins - pharmacology; Ligases; loss-of-function mutation; Nicotiana - drug effects; Nicotiana - enzymology; Nicotiana - genetics; Nicotiana - virology; Nicotiana benthamiana; Original; Plant Diseases - virology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants; polypeptides; Potexvirus - drug effects; Potexvirus - enzymology; Potexvirus - physiology; precipitin tests; Protein Binding - drug effects; Protein Stability - drug effects; protein‐protein interaction; protoplasts; RNA; RNA-Dependent RNA Polymerase - metabolism; RNA‐dependent RNA polymerase; Saccharomyces cerevisiae - metabolism; Subcellular Fractions - metabolism; two hybrid system techniques; Ubiquitin; ubiquitin-protein ligase; Ubiquitin-Protein Ligases - genetics; Ubiquitin-Protein Ligases - metabolism; Up-Regulation - drug effects; Up-Regulation - genetics; viral RNA replication; Virus Replication - drug effects; Virus Replication - physiology; yeast two‐hybrid; Nicotiana benthamiana; viral RNA replication; Bamboo mosaic virus; RNA-dependent RNA polymerase; yeast two-hybrid; E3 ubiquitin ligase; protein-protein interaction

Summary One up‐regulated host gene identified previously was found involved in the infection process of Bamboo mosaic virus (BaMV), a single‐stranded positive‐sense RNA virus. The full length cDNA of this gene was cloned by 5′ and 3′‐rapid amplification of cDNA ends and found to encode a polypeptide...