A key role of PGC‐1α transcriptional coactivator in production of VEGF by a novel angiogenic agent COA‐Cl in cultured human fibroblasts

• Published in: Physiological reports Vol. 4; no. 6; pp. e12742 - n/a
• Main Authors: Igarashi, Junsuke, Okamoto, Ryuji, Yamashita, Tetsuo, Hashimoto, Takeshi, Karita, Sakiko, Nakai, Kozo, Kubota, Yasuo, Takata, Maki, Yamaguchi, Fuminori, Tokuda, Masaaki, Sakakibara, Norikazu, Tsukamoto, Ikuko, Konishi, Ryoji, Hirano, Katsuya
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.03.2016; John Wiley and Sons Inc
• Subjects: Adenosine; Adenosine - analogs & derivatives; Adenosine - pharmacology; Angiogenesis; Angiogenesis Inducing Agents - pharmacology; Animals; Cardiovascular Conditions, Disorders and Treatments; Cellular Physiology; Chlorocebus aethiops; COS Cells; Dose-Response Relationship, Drug; Endothelial cells; Enzyme-linked immunosorbent assay; ERRalpha Estrogen-Related Receptor; Fibroblasts; Fibroblasts - drug effects; Fibroblasts - metabolism; Gene expression; gene regulation; Growth factors; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Ischemia; Menopause; Musculoskeletal system; nucleic acid; Original Research; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; PGC‐1α; Physiology; Proteins; Receptors, Estrogen - genetics; Receptors, Estrogen - metabolism; Regulatory Pathways; RNA Interference; RNA, Messenger - metabolism; Signal Transduction - drug effects; Signalling Pathways; siRNA; Skin; Time Factors; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription, Genetic; Transcriptional Activation; transcriptional coactivator; Transfection; Transplants & implants; Umbilical vein; Up-Regulation; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; VEGF; Japan; Angiogenesis; nucleic acid; transcriptional coactivator; gene regulation; VEGF; PGC‐1α

We previously demonstrated a potent angiogenic effect of a newly developed adenosine‐like agent named COA‐Cl. COA‐Cl exerted tube forming activity in human umbilical vein endothelial cells in the presence of normal human dermal fibroblasts (NHDF). We therefore explored whether and how COA‐Cl modulat...