Action Potential Morphology of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Does Not Predict Cardiac Chamber Specificity and Is Dependent on Cell Density

• Published in: Biophysical journal Vol. 108; no. 1; pp. 1 - 4
• Main Authors: Du, David T.M., Hellen, Nicola, Kane, Christopher, Terracciano, Cesare M.N.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.01.2015; Biophysical Society; The Biophysical Society
• Subjects: Action Potentials - drug effects; Action Potentials - physiology; Biophysical Letter; Carbenoxolone - pharmacology; Cardiomyocytes; Cardiovascular Agents - pharmacology; Cell Count; Cell culture; Cell Culture Techniques; Cells, Cultured; Gap Junctions - drug effects; Gap Junctions - physiology; Genotype & phenotype; Human subjects; Humans; Induced Pluripotent Stem Cells - drug effects; Induced Pluripotent Stem Cells - physiology; Linear Models; Morphology; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - physiology; Optical Imaging; Stem cells; Subpopulations

Previous studies investigating human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have proposed the distinction of heart chamber-specific (atrial, ventricular, pacemaker) electrophysiological phenotypes based on action potential (AP) morphology. This suggestion has been based on d...