Identification of a mutation causing a defective spindle assembly checkpoint in high ethyl caproate-producing sake yeast strain K1801

• Published in: Bioscience, biotechnology, and biochemistry Vol. 80; no. 8; pp. 1657 - 1662
• Main Authors: Goshima, Tetsuya, Nakamura, Ryo, Kume, Kazunori, Okada, Hiroki, Ichikawa, Eri, Tamura, Hiroyasu, Hasuda, Hirokazu, Inahashi, Masaaki, Okazaki, Naoto, Akao, Takeshi, Shimoi, Hitoshi, Mizunuma, Masaki, Ohya, Yoshikazu, Hirata, Dai
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.08.2016
• Subjects: Alcoholic Beverages; Amino Acid Sequence; Base Sequence; Caproates - metabolism; Cell Cycle Proteins - genetics; Cell Cycle Proteins - metabolism; cerulenin-resistant; checkpoint; Ethanol - metabolism; ethyl caproate; Fermentation; Food Technology; Gene Expression; Humans; Japan; M Phase Cell Cycle Checkpoints; morphological variation; Mutation; Odorants; Oryza - chemistry; Polymorphism, Single Nucleotide; Protein Phosphatase 2 - genetics; Protein Phosphatase 2 - metabolism; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; sake yeast; Selection, Genetic; checkpoint; ethyl caproate; cerulenin-resistant; sake yeast; morphological variation

In high-quality sake brewing, the cerulenin-resistant sake yeast K1801 with high ethyl caproate-producing ability has been used widely; however, K1801 has a defective spindle assembly checkpoint (SAC). To identify the mutation causing this defect, we first searched for sake yeasts with a SAC-defect...