Counteracting neuronal nitric oxide synthase proteasomal degradation improves glucose transport in insulin-resistant skeletal muscle from Zucker fa/fa rats

• Published in: Diabetologia Vol. 57; no. 1; pp. 177 - 186
• Main Authors: Mezghenna, Karima, Leroy, Jérémy, Azay-Milhau, Jacqueline, Tousch, Didier, Castex, Françoise, Gervais, Sylvain, Delgado-Betancourt, Viviana, Gross, René, Lajoix, Anne-Dominique
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2014; Springer; Springer Nature B.V; Springer Verlag
• Subjects: Animals; Biological and medical sciences; Blotting, Western; Cells, Cultured; Diabetes; Diabetes. Impaired glucose tolerance; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Enzymes; Etiopathogenesis. Screening. Investigations. Target tissue resistance; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - metabolism; Glucose Transporter Type 4 - metabolism; Human Physiology; Immunoprecipitation; Insulin resistance; Insulin Resistance - physiology; Internal Medicine; Life Sciences; Male; Medical sciences; Medicine; Medicine & Public Health; Metabolic Diseases; Muscle Cells - metabolism; Muscle, Skeletal - metabolism; Musculoskeletal system; Nitric oxide; Nitric Oxide Synthase Type I - genetics; Nitric Oxide Synthase Type I - metabolism; Obesity; Proteasome Endopeptidase Complex - metabolism; Proteins; Rats; Rats, Zucker; Reverse Transcriptase Polymerase Chain Reaction; Striated muscle. Tendons; Vertebrates: osteoarticular system, musculoskeletal system; United Kingdom > UK; France; Type 2 diabetes; NO synthase; Obesity; Insulin resistance; Skeletal muscle; Endocrinopathy; Pancreatic hormone; Rat; Enzyme; Biological transport; Rodentia; Nutrition disorder; Metabolic diseases; Glucose; Striated muscle; Insulin; Nitric-oxide synthase; Degradation; Target tissue resistance; Vertebrata; Mammalia; Animal; Oxidoreductases; Nutritional status

Aims/hypothesis Insulin-mediated glucose transport and utilisation are decreased in skeletal muscle from type 2 diabetic and glucose-intolerant individuals because of alterations in insulin receptor signalling, GLUT4 translocation to the plasma membrane and microvascular blood flow. Catalytic activi...