Generation of influenza A viruses as live but replication-incompetent virus vaccines

• Published in: Science (American Association for the Advancement of Science) Vol. 354; no. 6316; pp. 1170 - 1173
• Main Authors: Si, Longlong, Xu, Huan, Zhou, Xueying, Zhang, Ziwei, Tian, Zhenyu, Wang, Yan, Wu, Yiming, Zhang, Bo, Niu, Zhenlan, Zhang, Chuanling, Fu, Ge, Xiao, Sulong, Xia, Quig, Zhang, Lihe, Zhou, Demin
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 02.12.2016; The American Association for the Advancement of Science
• Subjects: Aluminum; Animals; Biotechnology; Codon, Nonsense; Ferrets; Genetic code; Genome, Viral; Genomics; Guinea Pigs; HEK293 Cells; Humans; Immunogenicity, Vaccine; Influenza; Influenza A virus; Influenza A virus - genetics; Influenza A virus - immunology; Influenza A virus - physiology; Influenza Vaccines - genetics; Influenza Vaccines - immunology; Methanosarcina barkeri - genetics; Mice; Models, Animal; Mustela; Orthomyxoviridae; Protein Biosynthesis - genetics; T-Lymphocytes - immunology; Transgenes; Transgenic; Vaccination; Vaccines; Vaccines, Attenuated - genetics; Vaccines, Attenuated - immunology; Viral Plaque Assay; Virus Cultivation - methods; Virus Replication - genetics; Viruses

The conversion of life-threatening viruses into live but avirulent vaccines represents a revolution in vaccinology. In a proof-of-principle study, we expanded the genetic code of the genome of influenza A virus via a transgenic cell line containing orthogonal translation machinery. This generated pr...