A humanized anti-human Fas antibody, R-125224, induces apoptosis in type I activated lymphocytes but not in type II cells

• Published in: International immunology Vol. 18; no. 1; pp. 113 - 124
• Main Authors: Nakayama, Junichi, Ogawa, Yukie, Yoshigae, Yasushi, Onozawa, Yoshiko, Yonemura, Akiko, Saito, Motoko, Ichikawa, Kimihisa, Yamoto, Takashi, Komai, Tomoaki, Tatsuta, Toru, Ohtsuki, Masahiko
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.01.2006; Oxford Publishing Limited (England)
• Subjects: 3′-dihexyloxacarbocyanine iodide; activated human lymphocytes; ALT alanine aminotransferase; Animals; anti-autoimmune disease therapy; anti-FLAG anti-FLAG M2 antibody; anti-hIgG anti-human IgG; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; APC antigen-presenting cell; Apoptosis - drug effects; Apoptosis - immunology; AST aspartate aminotransferase; Bmax maximum binding; CD3 Complex - immunology; CL collagen; CMTMR Cell Tracker™ Orange CMTMR; DiOC6 3; DISC; DISC death-inducing signaling complex; FADD Fas-associated death domain; fas Receptor - immunology; FasL Fas ligand; Hepatocytes - immunology; Humans; Immunologic Capping - drug effects; Immunologic Capping - immunology; Jurkat Cells; lipid rafts; Lymphocyte Activation - drug effects; Lymphocyte Activation - immunology; Mice; Mice, SCID; PI propidium iodide; RA rheumatoid arthritis; SCID-bg C·B-17/Icr Crj-scid bgBR; sFasL soluble Fas ligand; Signal Transduction - drug effects; Signal Transduction - immunology; Th1 Cells - immunology; Th2 Cells - immunology; TNF tumor necrosis factor; ΔΨm mitochondrial transmembrane potential

Fas-mediated apoptosis plays an important role in the immune system, including the elimination of autoreactive lymphoid cells. The Fas-mediated signaling pathway is classified into two types, type I and type II, in human lymphoid cell lines. We investigated whether a humanized anti-human Fas mAb, R-...