Transplantation of Adipose-Tissue-Engineered Constructs with CRISPR-Mediated UCP1 Activation

• Published in: International journal of molecular sciences Vol. 24; no. 4; p. 3844
• Main Authors: Michurina, Svetlana, Stafeev, Iurii, Boldyreva, Maria, Truong, Vu Anh, Ratner, Elizaveta, Menshikov, Mikhail, Hu, Yu-Chen, Parfyonova, Yelena
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 14.02.2023; MDPI
• Subjects: Adipocytes; Adipocytes, Brown - metabolism; Adipose Tissue, Brown - transplantation; Adipose tissues; Analysis; Animals; Body fat; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; Diabetes Mellitus, Type 2 - therapy; Efficiency; Energy consumption; Energy dissipation; Gene expression; Genes; Genetic engineering; Genomes; Glucose; Glucose - metabolism; Humans; Inflammation; Metabolism; Mice; Mice, Inbred C57BL; Morphology; Obesity; Scientific equipment and supplies industry; Technology application; Thermogenesis; Thermogenesis - genetics; Tissue engineering; Type 2 diabetes; Uncoupling Protein 1 - metabolism; United States; Massachusetts; Germany; Russia; CRISPR; thermogenesis; tissue engineering; adipocytes

Thermogenic adipocytes have potential utility for the development of approaches to treat type 2 diabetes and obesity-associated diseases. Although several reports have proved the positive effect of beige and brown adipocyte transplantation in obese mice, translation to human cell therapy needs impro...